| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 551,606 | G→A | 15.0% | L640L (CTG→TTG) | ybiW ← | predicted pyruvate formate lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 551,606 | 0 | G | A | 15.0% | 180.6 / 20.8 | 73 | L640L (CTG→TTG) | ybiW | predicted pyruvate formate lyase |
| Reads supporting (aligned to +/- strand): ref base G (33/29); new base A (5/6); total (38/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
ATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTT > minE/551537‑551675 | atgaCGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGt > 1:568524/1‑71 (MQ=255) tgaCGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGt < 1:3287217/70‑1 (MQ=255) aCGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcg > 1:3199100/1‑71 (MQ=255) gTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgtc > 1:2841901/1‑71 (MQ=255) gTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgtc > 1:690985/1‑71 (MQ=255) aCCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa > 1:2461637/1‑71 (MQ=255) cccGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:1406341/70‑1 (MQ=255) gTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgtcg < 1:1929855/64‑1 (MQ=255) aaTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTc > 1:299013/1‑71 (MQ=255) aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCt > 1:820950/1‑71 (MQ=255) aTAGTTGCCGCCAACCGGACAACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTc > 1:1056025/1‑70 (MQ=255) tAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTg < 1:3754005/71‑1 (MQ=255) gTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGAt < 1:2336962/71‑1 (MQ=255) ttGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATtcgtc < 1:1872860/56‑1 (MQ=255) tGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGAt > 1:546058/1‑69 (MQ=255) tGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATa < 1:3023056/70‑1 (MQ=255) gccgccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATaa < 1:2269083/70‑1 (MQ=255) gacgccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGa > 1:797005/3‑67 (MQ=255) ccgccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAg < 1:2839973/70‑1 (MQ=255) ccgccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATtcgtc > 1:2531550/1‑53 (MQ=255) ccgccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATtcgtc > 1:1226436/1‑53 (MQ=255) gccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAgcgc < 1:630789/71‑1 (MQ=255) gccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAgcgc > 1:1563408/1‑71 (MQ=255) gccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAgcgc < 1:266129/71‑1 (MQ=255) ccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAgcgc > 1:896987/1‑70 (MQ=255) ccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:2072533/1‑68 (MQ=255) cAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAgcgc > 1:3929534/1‑69 (MQ=255) aaCCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAg > 1:1799281/1‑71 (MQ=255) aaCCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAg > 1:965548/1‑71 (MQ=255) aCCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGc > 1:1636360/1‑71 (MQ=255) ccGGACCACGACCGTAGCGCGTATTATGGTACTGTTTCAGTTCGTCGATATAg > 1:220235/1‑53 (MQ=255) ccGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGc > 1:768676/1‑70 (MQ=255) ggACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATaa > 1:37049/1‑60 (MQ=255) gACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgtc < 1:1792764/43‑1 (MQ=255) aCCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCcagca < 1:1239914/71‑1 (MQ=255) aCCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCcagca < 1:1120119/71‑1 (MQ=255) aCGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGa > 1:3559369/1‑61 (MQ=255) cGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGatat > 1:451259/1‑43 (MQ=255) cGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATaa > 1:2553388/1‑54 (MQ=255) cGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGt < 1:480832/71‑1 (MQ=255) gACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTa < 1:1679345/71‑1 (MQ=255) aCCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATa < 1:3407214/51‑1 (MQ=255) ccGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATc > 1:231580/1‑71 (MQ=255) gTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGa < 1:212669/71‑1 (MQ=255) gTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGa < 1:3637067/71‑1 (MQ=255) tAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGa > 1:988280/1‑70 (MQ=255) tAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGa > 1:2956162/1‑70 (MQ=255) tAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGa > 1:1599210/1‑70 (MQ=255) gcgcGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACa < 1:553460/70‑1 (MQ=255) gcgcGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGAAGCGTATCGACAg < 1:3852110/71‑1 (MQ=255) cgcgGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAg < 1:1513443/70‑1 (MQ=255) cgcgGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGt < 1:2121937/71‑1 (MQ=255) cgcgGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGa < 1:307639/51‑1 (MQ=255) ggATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTAt > 1:3364302/1‑61 (MQ=255) gATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTatca > 1:149624/1‑71 (MQ=255) tACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGtt < 1:1118933/71‑1 (MQ=255) tACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGtt < 1:3392097/71‑1 (MQ=255) tACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGtt < 1:765023/71‑1 (MQ=255) tACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGtt > 1:638977/1‑71 (MQ=255) aCTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGa > 1:36055/1‑54 (MQ=255) tGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTatca > 1:2315831/1‑60 (MQ=255) tGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGcc > 1:894711/1‑71 (MQ=255) gTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACa > 1:719086/1‑53 (MQ=255) gTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGa < 1:674927/51‑1 (MQ=255) gTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGa < 1:1593032/51‑1 (MQ=255) tttCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACa < 1:394473/52‑1 (MQ=255) tttCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCg < 1:2749467/70‑1 (MQ=255) tttCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCg < 1:2754120/70‑1 (MQ=255) tttCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCg < 1:3131170/70‑1 (MQ=255) tttCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGt < 1:3256480/71‑1 (MQ=255) ttCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGt > 1:1889762/1‑70 (MQ=255) ttCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTa > 1:2448938/1‑71 (MQ=255) aGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACtt < 1:1307973/71‑1 (MQ=255) | ATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTT > minE/551537‑551675 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |