Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 608,004 G→A 7.1% D750D (GAC→GAT)  pflB ← pyruvate formate lyase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE608,0040GA 7.1% 213.4 / 4.8 70D750D (GAC→GAT) pflBpyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base G (35/30);  new base A (2/3);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

CTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCT  >  minE/607940‑608071
                                                                |                                                                   
cTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCctgc                                                               >  1:1415794/1‑71 (MQ=255)
   tttACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCctgct                                                              <  1:1048501/69‑1 (MQ=255)
     tACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCctgctgctg                                                          >  1:3917770/1‑71 (MQ=255)
        tGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTc                                                       >  1:2297901/1‑71 (MQ=255)
         gTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCt                                                      >  1:1058054/1‑71 (MQ=255)
          tACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATACCGTCctgc                                                               <  1:144784/61‑1 (MQ=255)
           aCGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCtt                                                     >  1:1419045/1‑70 (MQ=255)
            cGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCtt                                                     >  1:2149110/1‑69 (MQ=255)
              aTTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGt                                                 <  1:645465/71‑1 (MQ=255)
               tttCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGt                                                 >  1:3718734/1‑70 (MQ=255)
                ttCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGt                                                 >  1:160869/1‑69 (MQ=255)
                 tCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAg                                              >  1:996873/1‑71 (MQ=255)
                  cAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGt                                                         <  1:1741181/59‑1 (MQ=255)
                   aGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGc                                             >  1:2485275/1‑70 (MQ=255)
                   aGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGc                                             >  1:3260389/1‑70 (MQ=255)
                    gTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGa                                           <  1:1089431/71‑1 (MQ=255)
                       aaaTCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGt                                                 >  1:2287770/1‑62 (MQ=255)
                       aaaTCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGtt                                        >  1:662524/1‑71 (MQ=255)
                        aaTCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAg                                                  <  1:296126/60‑1 (MQ=255)
                          tCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTc                                                                   >  1:462362/1‑41 (MQ=255)
                          tCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTc                                                                   >  1:2378988/1‑41 (MQ=255)
                            tAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAg                                                  <  1:2293648/56‑1 (MQ=255)
                            tAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAg                                                  <  1:2890959/56‑1 (MQ=255)
                            tAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGaa                                     >  1:2061563/1‑69 (MQ=255)
                             aaTTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGATGTTCTTTAGTCAGcca                                           >  1:3790467/1‑60 (MQ=255)
                              aTTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGaaa                                    <  1:3017429/68‑1 (MQ=255)
                               ttACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTa                                >  1:34797/1‑71 (MQ=255)
                               ttACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTa                                <  1:2432002/71‑1 (MQ=255)
                                 aCATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTAc                               <  1:1389128/70‑1 (MQ=255)
                                  cATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTg                             >  1:1887533/1‑71 (MQ=255)
                                   aTAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGc                            >  1:3546040/1‑71 (MQ=255)
                                    tAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCg                           <  1:616304/71‑1 (MQ=255)
                                     aGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCtt                                                     >  1:1536325/1‑44 (MQ=255)
                                         tGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGaaa                                    >  1:3509632/1‑57 (MQ=255)
                                         tGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAga                       >  1:2313334/1‑69 (MQ=255)
                                         tGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGc                       >  1:2898512/1‑70 (MQ=255)
                                          gAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAAc                                   >  1:2602647/1‑57 (MQ=255)
                                          gAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAAc                                   >  1:1709618/1‑57 (MQ=255)
                                           aGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTTTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCa                     <  1:2450779/70‑1 (MQ=255)
                                           aGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTTTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCa                     <  1:2455529/70‑1 (MQ=255)
                                            gtaaaGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGa                   <  1:3531908/68‑1 (MQ=255)
                                            gTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGcc                      <  1:3339913/68‑1 (MQ=255)
                                             tGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCtt                                                     <  1:3852777/36‑1 (MQ=255)
                                             tGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGa                   >  1:3822705/1‑70 (MQ=255)
                                               aaGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGaaa                                    <  1:3075954/51‑1 (MQ=255)
                                               aaGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATac                <  1:2826648/71‑1 (MQ=255)
                                                aGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATac                <  1:3332902/70‑1 (MQ=255)
                                                  gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg             >  1:2028385/1‑71 (MQ=255)
                                                  gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg             <  1:3737148/71‑1 (MQ=255)
                                                  gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg             <  1:2535925/71‑1 (MQ=255)
                                                  gTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGa                                           <  1:1477452/41‑1 (MQ=255)
                                                   tACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACgg            <  1:3117734/71‑1 (MQ=255)
                                                   tACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACgg            <  1:283522/71‑1 (MQ=255)
                                                     cGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGa           <  1:827193/70‑1 (MQ=255)
                                                     cGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGa           <  1:2488753/70‑1 (MQ=255)
                                                      gAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAg                                          >  1:1576436/1‑38 (MQ=255)
                                                      gAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGAt          >  1:591593/1‑70 (MQ=255)
                                                        gaaataaCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGt       >  1:1494929/3‑71 (MQ=255)
                                                        gTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt       >  1:964003/1‑71 (MQ=255)
                                                        gTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt       >  1:580131/1‑71 (MQ=255)
                                                         taataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTg                                       <  1:3178103/38‑1 (MQ=255)
                                                         taataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTg                                       <  1:2649351/38‑1 (MQ=255)
                                                         taataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt       >  1:2108558/1‑70 (MQ=255)
                                                          aataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATaca               >  1:2941795/1‑61 (MQ=255)
                                                           ataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAg    >  1:1002818/1‑71 (MQ=255)
                                                           ataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAg    >  1:1282906/1‑71 (MQ=255)
                                                            taaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCg                           <  1:3021267/47‑1 (MQ=255)
                                                              aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt  <  1:2502981/70‑1 (MQ=255)
                                                              aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt  <  1:264757/70‑1 (MQ=255)
                                                              aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt  <  1:2694977/70‑1 (MQ=255)
                                                                gTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATaca               >  1:2011968/1‑55 (MQ=255)
                                                                |                                                                   
CTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCT  >  minE/607940‑608071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑