Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 608,010 C→T 7.4% Q748Q (CAG→CAA)  pflB ← pyruvate formate lyase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE608,0100CT 7.4% 201.2 / 4.9 67Q748Q (CAG→CAA) pflBpyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base C (33/29);  new base T (2/3);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

CTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGA  >  minE/607940‑608076
                                                                      |                                                                  
cTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCctgc                                                                    >  1:1415794/1‑71 (MQ=255)
   tttACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCctgct                                                                   <  1:1048501/69‑1 (MQ=255)
     tACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCctgctgctg                                                               >  1:3917770/1‑71 (MQ=255)
        tGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTc                                                            >  1:2297901/1‑71 (MQ=255)
         gTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCt                                                           >  1:1058054/1‑71 (MQ=255)
          tACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATACCGTCctgc                                                                    <  1:144784/61‑1 (MQ=255)
           aCGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCtt                                                          >  1:1419045/1‑70 (MQ=255)
            cGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCtt                                                          >  1:2149110/1‑69 (MQ=255)
              aTTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGt                                                      <  1:645465/71‑1 (MQ=255)
               tttCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGt                                                      >  1:3718734/1‑70 (MQ=255)
                ttCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGt                                                      >  1:160869/1‑69 (MQ=255)
                 tCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAg                                                   >  1:996873/1‑71 (MQ=255)
                  cAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGt                                                              <  1:1741181/59‑1 (MQ=255)
                   aGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGc                                                  >  1:3260389/1‑70 (MQ=255)
                   aGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGc                                                  >  1:2485275/1‑70 (MQ=255)
                    gTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGa                                                <  1:1089431/71‑1 (MQ=255)
                       aaaTCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGt                                                      >  1:2287770/1‑62 (MQ=255)
                       aaaTCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGtt                                             >  1:662524/1‑71 (MQ=255)
                        aaTCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAg                                                       <  1:296126/60‑1 (MQ=255)
                            tAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAg                                                       <  1:2890959/56‑1 (MQ=255)
                            tAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAg                                                       <  1:2293648/56‑1 (MQ=255)
                            tAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGaa                                          >  1:2061563/1‑69 (MQ=255)
                             aaTTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGATGTTCTTTAGTCAGcca                                                >  1:3790467/1‑60 (MQ=255)
                              aTTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGaaa                                         <  1:3017429/68‑1 (MQ=255)
                               ttACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTa                                     >  1:34797/1‑71 (MQ=255)
                               ttACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTa                                     <  1:2432002/71‑1 (MQ=255)
                                 aCATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTAc                                    <  1:1389128/70‑1 (MQ=255)
                                  cATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTg                                  >  1:1887533/1‑71 (MQ=255)
                                   aTAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGc                                 >  1:3546040/1‑71 (MQ=255)
                                    tAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCg                                <  1:616304/71‑1 (MQ=255)
                                     aGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCtt                                                          >  1:1536325/1‑44 (MQ=255)
                                         tGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGaaa                                         >  1:3509632/1‑57 (MQ=255)
                                         tGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAga                            >  1:2313334/1‑69 (MQ=255)
                                         tGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGc                            >  1:2898512/1‑70 (MQ=255)
                                          gAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAAc                                        >  1:2602647/1‑57 (MQ=255)
                                          gAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAAc                                        >  1:1709618/1‑57 (MQ=255)
                                           aGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTTTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCa                          <  1:2455529/70‑1 (MQ=255)
                                           aGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTTTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCa                          <  1:2450779/70‑1 (MQ=255)
                                            gtaaaGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGa                        <  1:3531908/68‑1 (MQ=255)
                                            gTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGcc                           <  1:3339913/68‑1 (MQ=255)
                                             tGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCtt                                                          <  1:3852777/36‑1 (MQ=255)
                                             tGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGa                        >  1:3822705/1‑70 (MQ=255)
                                               aaGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGaaa                                         <  1:3075954/51‑1 (MQ=255)
                                               aaGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATac                     <  1:2826648/71‑1 (MQ=255)
                                                aGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATac                     <  1:3332902/70‑1 (MQ=255)
                                                  gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                  <  1:2535925/71‑1 (MQ=255)
                                                  gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                  <  1:3737148/71‑1 (MQ=255)
                                                  gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                  >  1:2028385/1‑71 (MQ=255)
                                                  gTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGa                                                <  1:1477452/41‑1 (MQ=255)
                                                   tACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACgg                 <  1:3117734/71‑1 (MQ=255)
                                                   tACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACgg                 <  1:283522/71‑1 (MQ=255)
                                                     cGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGa                <  1:827193/70‑1 (MQ=255)
                                                     cGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGa                <  1:2488753/70‑1 (MQ=255)
                                                      gAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAg                                               >  1:1576436/1‑38 (MQ=255)
                                                      gAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGAt               >  1:591593/1‑70 (MQ=255)
                                                        gaaataaCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGt            >  1:1494929/3‑71 (MQ=255)
                                                        gTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt            >  1:580131/1‑71 (MQ=255)
                                                        gTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt            >  1:964003/1‑71 (MQ=255)
                                                         taataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTg                                            <  1:3178103/38‑1 (MQ=255)
                                                         taataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTg                                            <  1:2649351/38‑1 (MQ=255)
                                                         taataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt            >  1:2108558/1‑70 (MQ=255)
                                                          aataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATaca                    >  1:2941795/1‑61 (MQ=255)
                                                           ataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAg         >  1:1282906/1‑71 (MQ=255)
                                                           ataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAg         >  1:1002818/1‑71 (MQ=255)
                                                            taaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCg                                <  1:3021267/47‑1 (MQ=255)
                                                              aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt       <  1:2694977/70‑1 (MQ=255)
                                                              aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt       <  1:264757/70‑1 (MQ=255)
                                                              aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt       <  1:2502981/70‑1 (MQ=255)
                                                                gTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATaca                    >  1:2011968/1‑55 (MQ=255)
                                                                  ccTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACg                  <  1:705950/55‑1 (MQ=255)
                                                                  ccTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa  >  1:145123/1‑71 (MQ=255)
                                                                   ctgctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa  <  1:910165/70‑1 (MQ=255)
                                                                     gctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGa                        >  1:33633/1‑46 (MQ=255)
                                                                      |                                                                  
CTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGA  >  minE/607940‑608076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑