Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 608,020 T→C 7.3% K745R (AAA→AGA) ‡ pflB ← pyruvate formate lyase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE608,0200TC 7.3% 191.3 / 5.3 68K745R (AAA→AGA) ‡pflBpyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base T (32/31);  new base C (2/3);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

ACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCC  >  minE/607951‑608085
                                                                     |                                                                 
aCGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCtt                                                                   >  1:1419045/1‑70 (MQ=255)
 cGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCtt                                                                   >  1:2149110/1‑69 (MQ=255)
   aTTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGt                                                               <  1:645465/71‑1 (MQ=255)
    tttCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGt                                                               >  1:3718734/1‑70 (MQ=255)
     ttCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGt                                                               >  1:160869/1‑69 (MQ=255)
      tCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAg                                                            >  1:996873/1‑71 (MQ=255)
        aGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGc                                                           >  1:3260389/1‑70 (MQ=255)
        aGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGc                                                           >  1:2485275/1‑70 (MQ=255)
         gTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGa                                                         <  1:1089431/71‑1 (MQ=255)
            aaaTCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGt                                                               >  1:2287770/1‑62 (MQ=255)
            aaaTCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGtt                                                      >  1:662524/1‑71 (MQ=255)
             aaTCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAg                                                                <  1:296126/60‑1 (MQ=255)
                 tAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAg                                                                <  1:2890959/56‑1 (MQ=255)
                 tAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAg                                                                <  1:2293648/56‑1 (MQ=255)
                 tAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGaa                                                   >  1:2061563/1‑69 (MQ=255)
                  aaTTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGATGTTCTTTAGTCAGcca                                                         >  1:3790467/1‑60 (MQ=255)
                   aTTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGaaa                                                  <  1:3017429/68‑1 (MQ=255)
                    ttACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTa                                              >  1:34797/1‑71 (MQ=255)
                    ttACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTa                                              <  1:2432002/71‑1 (MQ=255)
                      aCATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTAc                                             <  1:1389128/70‑1 (MQ=255)
                       cATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTg                                           >  1:1887533/1‑71 (MQ=255)
                        aTAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGc                                          >  1:3546040/1‑71 (MQ=255)
                         tAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCg                                         <  1:616304/71‑1 (MQ=255)
                          aGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCtt                                                                   >  1:1536325/1‑44 (MQ=255)
                              tGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGaaa                                                  >  1:3509632/1‑57 (MQ=255)
                              tGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAga                                     >  1:2313334/1‑69 (MQ=255)
                              tGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGc                                     >  1:2898512/1‑70 (MQ=255)
                               gAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAAc                                                 >  1:1709618/1‑57 (MQ=255)
                               gAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAAc                                                 >  1:2602647/1‑57 (MQ=255)
                                aGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTTTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCa                                   <  1:2455529/70‑1 (MQ=255)
                                aGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTTTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCa                                   <  1:2450779/70‑1 (MQ=255)
                                 gtaaaGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGa                                 <  1:3531908/68‑1 (MQ=255)
                                 gTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGcc                                    <  1:3339913/68‑1 (MQ=255)
                                  tGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCtt                                                                   <  1:3852777/36‑1 (MQ=255)
                                  tGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGa                                 >  1:3822705/1‑70 (MQ=255)
                                    aaGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGaaa                                                  <  1:3075954/51‑1 (MQ=255)
                                    aaGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATac                              <  1:2826648/71‑1 (MQ=255)
                                     aGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATac                              <  1:3332902/70‑1 (MQ=255)
                                       gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                           <  1:3737148/71‑1 (MQ=255)
                                       gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                           >  1:2028385/1‑71 (MQ=255)
                                       gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                           <  1:2535925/71‑1 (MQ=255)
                                       gTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGa                                                         <  1:1477452/41‑1 (MQ=255)
                                        tACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACgg                          <  1:283522/71‑1 (MQ=255)
                                        tACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACgg                          <  1:3117734/71‑1 (MQ=255)
                                          cGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGa                         <  1:827193/70‑1 (MQ=255)
                                          cGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGa                         <  1:2488753/70‑1 (MQ=255)
                                           gAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAg                                                        >  1:1576436/1‑38 (MQ=255)
                                           gAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGAt                        >  1:591593/1‑70 (MQ=255)
                                             gaaataaCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGt                     >  1:1494929/3‑71 (MQ=255)
                                             gTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                     >  1:580131/1‑71 (MQ=255)
                                             gTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                     >  1:964003/1‑71 (MQ=255)
                                              taataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTg                                                     <  1:3178103/38‑1 (MQ=255)
                                              taataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTg                                                     <  1:2649351/38‑1 (MQ=255)
                                              taataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                     >  1:2108558/1‑70 (MQ=255)
                                               aataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATaca                             >  1:2941795/1‑61 (MQ=255)
                                                ataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAg                  >  1:1002818/1‑71 (MQ=255)
                                                ataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAg                  >  1:1282906/1‑71 (MQ=255)
                                                 taaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCg                                         <  1:3021267/47‑1 (MQ=255)
                                                   aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt                <  1:2694977/70‑1 (MQ=255)
                                                   aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt                <  1:264757/70‑1 (MQ=255)
                                                   aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt                <  1:2502981/70‑1 (MQ=255)
                                                     gTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATaca                             >  1:2011968/1‑55 (MQ=255)
                                                       ccTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACg                           <  1:705950/55‑1 (MQ=255)
                                                       ccTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa           >  1:145123/1‑71 (MQ=255)
                                                        ctgctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa           <  1:910165/70‑1 (MQ=255)
                                                          gctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGa                                 >  1:33633/1‑46 (MQ=255)
                                                            tgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttt      >  1:1300684/1‑71 (MQ=255)
                                                            tgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttt      >  1:3792193/1‑71 (MQ=255)
                                                              ctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATg                       >  1:2330520/1‑52 (MQ=255)
                                                                 ttCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc  <  1:2198588/70‑1 (MQ=255)
                                                                 ttCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc  <  1:2531698/70‑1 (MQ=255)
                                                                 ttCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc  <  1:1404730/70‑1 (MQ=255)
                                                                     |                                                                 
ACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCC  >  minE/607951‑608085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑