Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 608,021 T→G 7.4% K745Q (AAA→CAA) ‡ pflB ← pyruvate formate lyase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE608,0210TG 7.4% 171.7 / 8.1 68K745Q (AAA→CAA) ‡pflBpyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base T (32/31);  new base G (2/3);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

ATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTT  >  minE/607954‑608091
                                                                   |                                                                      
aTTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGt                                                                     <  1:645465/71‑1 (MQ=255)
 tttCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGt                                                                     >  1:3718734/1‑70 (MQ=255)
  ttCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGt                                                                     >  1:160869/1‑69 (MQ=255)
   tCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAg                                                                  >  1:996873/1‑71 (MQ=255)
     aGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGc                                                                 >  1:3260389/1‑70 (MQ=255)
     aGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGc                                                                 >  1:2485275/1‑70 (MQ=255)
      gTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGa                                                               <  1:1089431/71‑1 (MQ=255)
         aaaTCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGt                                                                     >  1:2287770/1‑62 (MQ=255)
         aaaTCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGtt                                                            >  1:662524/1‑71 (MQ=255)
          aaTCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAg                                                                      <  1:296126/60‑1 (MQ=255)
              tAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAg                                                                      <  1:2890959/56‑1 (MQ=255)
              tAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAg                                                                      <  1:2293648/56‑1 (MQ=255)
              tAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGaa                                                         >  1:2061563/1‑69 (MQ=255)
               aaTTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGATGTTCTTTAGTCAGcca                                                               >  1:3790467/1‑60 (MQ=255)
                aTTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGaaa                                                        <  1:3017429/68‑1 (MQ=255)
                 ttACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTa                                                    >  1:34797/1‑71 (MQ=255)
                 ttACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTa                                                    <  1:2432002/71‑1 (MQ=255)
                   aCATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTAc                                                   <  1:1389128/70‑1 (MQ=255)
                    cATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTg                                                 >  1:1887533/1‑71 (MQ=255)
                     aTAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGc                                                >  1:3546040/1‑71 (MQ=255)
                      tAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCg                                               <  1:616304/71‑1 (MQ=255)
                           tGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGaaa                                                        >  1:3509632/1‑57 (MQ=255)
                           tGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAga                                           >  1:2313334/1‑69 (MQ=255)
                           tGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGc                                           >  1:2898512/1‑70 (MQ=255)
                            gAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAAc                                                       >  1:1709618/1‑57 (MQ=255)
                            gAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAAc                                                       >  1:2602647/1‑57 (MQ=255)
                             aGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTTTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCa                                         <  1:2450779/70‑1 (MQ=255)
                             aGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTTTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCa                                         <  1:2455529/70‑1 (MQ=255)
                              gtaaaGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGa                                       <  1:3531908/68‑1 (MQ=255)
                              gTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGcc                                          <  1:3339913/68‑1 (MQ=255)
                               tGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGa                                       >  1:3822705/1‑70 (MQ=255)
                                 aaGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGaaa                                                        <  1:3075954/51‑1 (MQ=255)
                                 aaGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATac                                    <  1:2826648/71‑1 (MQ=255)
                                  aGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATac                                    <  1:3332902/70‑1 (MQ=255)
                                    gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                                 <  1:2535925/71‑1 (MQ=255)
                                    gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                                 <  1:3737148/71‑1 (MQ=255)
                                    gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                                 >  1:2028385/1‑71 (MQ=255)
                                    gTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGa                                                               <  1:1477452/41‑1 (MQ=255)
                                     tACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACgg                                <  1:283522/71‑1 (MQ=255)
                                     tACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACgg                                <  1:3117734/71‑1 (MQ=255)
                                       cGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGa                               <  1:2488753/70‑1 (MQ=255)
                                       cGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGa                               <  1:827193/70‑1 (MQ=255)
                                        gAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAg                                                              >  1:1576436/1‑38 (MQ=255)
                                        gAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGAt                              >  1:591593/1‑70 (MQ=255)
                                          gaaataaCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGt                           >  1:1494929/3‑71 (MQ=255)
                                          gTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                           >  1:580131/1‑71 (MQ=255)
                                          gTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                           >  1:964003/1‑71 (MQ=255)
                                           taataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTg                                                           <  1:2649351/38‑1 (MQ=255)
                                           taataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTg                                                           <  1:3178103/38‑1 (MQ=255)
                                           taataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                           >  1:2108558/1‑70 (MQ=255)
                                            aataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATaca                                   >  1:2941795/1‑61 (MQ=255)
                                             ataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAg                        >  1:1002818/1‑71 (MQ=255)
                                             ataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAg                        >  1:1282906/1‑71 (MQ=255)
                                              taaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCg                                               <  1:3021267/47‑1 (MQ=255)
                                                aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt                      <  1:2694977/70‑1 (MQ=255)
                                                aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt                      <  1:264757/70‑1 (MQ=255)
                                                aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt                      <  1:2502981/70‑1 (MQ=255)
                                                  gTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATaca                                   >  1:2011968/1‑55 (MQ=255)
                                                    ccTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACg                                 <  1:705950/55‑1 (MQ=255)
                                                    ccTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa                 >  1:145123/1‑71 (MQ=255)
                                                     ctgctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa                 <  1:910165/70‑1 (MQ=255)
                                                       gctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGa                                       >  1:33633/1‑46 (MQ=255)
                                                         tgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttt            >  1:1300684/1‑71 (MQ=255)
                                                         tgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttt            >  1:3792193/1‑71 (MQ=255)
                                                           ctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATg                             >  1:2330520/1‑52 (MQ=255)
                                                              ttCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc        <  1:1404730/70‑1 (MQ=255)
                                                              ttCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc        <  1:2198588/70‑1 (MQ=255)
                                                              ttCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc        <  1:2531698/70‑1 (MQ=255)
                                                                   tAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                           >  1:3532970/1‑46 (MQ=255)
                                                                   tAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGttt  >  1:941510/1‑71 (MQ=255)
                                                                   |                                                                      
ATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTT  >  minE/607954‑608091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑