Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,030	A→C	8.1%	S742A (TCG→GCG) ‡	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,030	0	A	C	8.1%	151.8 / 8.2	62	S742A (TCG→GCG) ‡	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base A (30/27);  new base C (2/3);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

GTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCAT  >  minE/607960‑608096                                                                       |                                                                   gTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGa                                                                    <  1:1089431/71‑1 (MQ=255)    aaaTCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGtt                                                                 >  1:662524/1‑71 (MQ=255)         tAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGaa                                                              >  1:2061563/1‑69 (MQ=255)           aTTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGaaa                                                             <  1:3017429/68‑1 (MQ=255)            ttACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTa                                                         >  1:34797/1‑71 (MQ=255)            ttACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTa                                                         <  1:2432002/71‑1 (MQ=255)              aCATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTAc                                                        <  1:1389128/70‑1 (MQ=255)               cATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTg                                                      >  1:1887533/1‑71 (MQ=255)                aTAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGc                                                     >  1:3546040/1‑71 (MQ=255)                 tAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCg                                                    <  1:616304/71‑1 (MQ=255)                      tGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGaaa                                                             >  1:3509632/1‑57 (MQ=255)                      tGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAga                                                >  1:2313334/1‑69 (MQ=255)                      tGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGc                                                >  1:2898512/1‑70 (MQ=255)                       gAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAAc                                                            >  1:1709618/1‑57 (MQ=255)                       gAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAAc                                                            >  1:2602647/1‑57 (MQ=255)                        aGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTTTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCa                                              <  1:2450779/70‑1 (MQ=255)                        aGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTTTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCa                                              <  1:2455529/70‑1 (MQ=255)                         gtaaaGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGa                                            <  1:3531908/68‑1 (MQ=255)                         gTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGcc                                               <  1:3339913/68‑1 (MQ=255)                          tGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGa                                            >  1:3822705/1‑70 (MQ=255)                            aaGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGaaa                                                             <  1:3075954/51‑1 (MQ=255)                            aaGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATac                                         <  1:2826648/71‑1 (MQ=255)                             aGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATac                                         <  1:3332902/70‑1 (MQ=255)                               gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                                      <  1:2535925/71‑1 (MQ=255)                               gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                                      <  1:3737148/71‑1 (MQ=255)                               gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                                      >  1:2028385/1‑71 (MQ=255)                               gTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGa                                                                    <  1:1477452/41‑1 (MQ=255)                                tACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACgg                                     <  1:3117734/71‑1 (MQ=255)                                tACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACgg                                     <  1:283522/71‑1 (MQ=255)                                  cGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGa                                    <  1:2488753/70‑1 (MQ=255)                                  cGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGa                                    <  1:827193/70‑1 (MQ=255)                                   gAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAg                                                                   >  1:1576436/1‑38 (MQ=255)                                   gAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGAt                                   >  1:591593/1‑70 (MQ=255)                                     gaaataaCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGt                                >  1:1494929/3‑71 (MQ=255)                                     gTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                                >  1:964003/1‑71 (MQ=255)                                     gTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                                >  1:580131/1‑71 (MQ=255)                                      taataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTg                                                                <  1:2649351/38‑1 (MQ=255)                                      taataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTg                                                                <  1:3178103/38‑1 (MQ=255)                                      taataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                                >  1:2108558/1‑70 (MQ=255)                                       aataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATaca                                        >  1:2941795/1‑61 (MQ=255)                                        ataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAg                             >  1:1002818/1‑71 (MQ=255)                                        ataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAg                             >  1:1282906/1‑71 (MQ=255)                                         taaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCg                                                    <  1:3021267/47‑1 (MQ=255)                                           aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt                           <  1:2694977/70‑1 (MQ=255)                                           aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt                           <  1:2502981/70‑1 (MQ=255)                                           aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt                           <  1:264757/70‑1 (MQ=255)                                             gTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATaca                                        >  1:2011968/1‑55 (MQ=255)                                               ccTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACg                                      <  1:705950/55‑1 (MQ=255)                                               ccTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa                      >  1:145123/1‑71 (MQ=255)                                                ctgctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa                      <  1:910165/70‑1 (MQ=255)                                                  gctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGa                                            >  1:33633/1‑46 (MQ=255)                                                    tgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttt                 >  1:3792193/1‑71 (MQ=255)                                                    tgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttt                 >  1:1300684/1‑71 (MQ=255)                                                      ctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATg                                  >  1:2330520/1‑52 (MQ=255)                                                         ttCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc             <  1:2531698/70‑1 (MQ=255)                                                         ttCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc             <  1:1404730/70‑1 (MQ=255)                                                         ttCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc             <  1:2198588/70‑1 (MQ=255)                                                              tAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                                >  1:3532970/1‑46 (MQ=255)                                                              tAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGttt       >  1:941510/1‑71 (MQ=255)                                                                 tCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc             <  1:2401492/62‑1 (MQ=255)                                                                   aGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGAt                                   >  1:2091158/1‑38 (MQ=255)                                                                   aGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGAt                                   >  1:1210512/1‑38 (MQ=255)                                                                   aGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCAt  >  1:1828542/1‑71 (MQ=255)                                                                     cGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTg                          <  1:2570363/45‑1 (MQ=255)                                                                       |                                                                   GTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCAT  >  minE/607960‑608096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑