Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,037	A→G	8.5%	R739R (CGT→CGC)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,037	0	A	G	8.5%	159.3 / 5.7	59	R739R (CGT→CGC)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base A (30/24);  new base G (2/3);  total (32/27)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

ATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAG  >  minE/607970‑608105                                                                    |                                                                     aTTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGaaa                                                                      <  1:3017429/68‑1 (MQ=255)  ttACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTa                                                                  >  1:34797/1‑71 (MQ=255)  ttACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTa                                                                  <  1:2432002/71‑1 (MQ=255)    aCATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTAc                                                                 <  1:1389128/70‑1 (MQ=255)     cATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTg                                                               >  1:1887533/1‑71 (MQ=255)      aTAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGc                                                              >  1:3546040/1‑71 (MQ=255)       tAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCg                                                             <  1:616304/71‑1 (MQ=255)            tGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGaaa                                                                      >  1:3509632/1‑57 (MQ=255)            tGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAga                                                         >  1:2313334/1‑69 (MQ=255)            tGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGc                                                         >  1:2898512/1‑70 (MQ=255)             gAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAAc                                                                     >  1:1709618/1‑57 (MQ=255)             gAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAAc                                                                     >  1:2602647/1‑57 (MQ=255)              aGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTTTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCa                                                       <  1:2450779/70‑1 (MQ=255)              aGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTTTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCa                                                       <  1:2455529/70‑1 (MQ=255)               gtaaaGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGa                                                     <  1:3531908/68‑1 (MQ=255)               gTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGcc                                                        <  1:3339913/68‑1 (MQ=255)                tGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGa                                                     >  1:3822705/1‑70 (MQ=255)                  aaGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGaaa                                                                      <  1:3075954/51‑1 (MQ=255)                  aaGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATac                                                  <  1:2826648/71‑1 (MQ=255)                   aGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATac                                                  <  1:3332902/70‑1 (MQ=255)                     gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                                               <  1:2535925/71‑1 (MQ=255)                     gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                                               >  1:2028385/1‑71 (MQ=255)                     gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                                               <  1:3737148/71‑1 (MQ=255)                      tACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACgg                                              <  1:3117734/71‑1 (MQ=255)                      tACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACgg                                              <  1:283522/71‑1 (MQ=255)                        cGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGa                                             <  1:2488753/70‑1 (MQ=255)                        cGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGa                                             <  1:827193/70‑1 (MQ=255)                         gAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGAt                                            >  1:591593/1‑70 (MQ=255)                           gaaataaCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGt                                         >  1:1494929/3‑71 (MQ=255)                           gTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                                         >  1:580131/1‑71 (MQ=255)                           gTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                                         >  1:964003/1‑71 (MQ=255)                            taataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                                         >  1:2108558/1‑70 (MQ=255)                             aataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATaca                                                 >  1:2941795/1‑61 (MQ=255)                              ataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAg                                      >  1:1002818/1‑71 (MQ=255)                              ataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAg                                      >  1:1282906/1‑71 (MQ=255)                               taaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCg                                                             <  1:3021267/47‑1 (MQ=255)                                 aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt                                    <  1:2502981/70‑1 (MQ=255)                                 aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt                                    <  1:264757/70‑1 (MQ=255)                                 aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt                                    <  1:2694977/70‑1 (MQ=255)                                   gTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATaca                                                 >  1:2011968/1‑55 (MQ=255)                                     ccTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACg                                               <  1:705950/55‑1 (MQ=255)                                     ccTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa                               >  1:145123/1‑71 (MQ=255)                                      ctgctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa                               <  1:910165/70‑1 (MQ=255)                                        gctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGa                                                     >  1:33633/1‑46 (MQ=255)                                          tgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttt                          >  1:3792193/1‑71 (MQ=255)                                          tgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttt                          >  1:1300684/1‑71 (MQ=255)                                            ctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATg                                           >  1:2330520/1‑52 (MQ=255)                                               ttCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc                      <  1:2198588/70‑1 (MQ=255)                                               ttCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc                      <  1:1404730/70‑1 (MQ=255)                                               ttCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc                      <  1:2531698/70‑1 (MQ=255)                                                    tAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                                         >  1:3532970/1‑46 (MQ=255)                                                    tAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGttt                >  1:941510/1‑71 (MQ=255)                                                       tCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc                      <  1:2401492/62‑1 (MQ=255)                                                         aGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGAt                                            >  1:2091158/1‑38 (MQ=255)                                                         aGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGAt                                            >  1:1210512/1‑38 (MQ=255)                                                         aGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCAt           >  1:1828542/1‑71 (MQ=255)                                                           cGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTg                                   <  1:2570363/45‑1 (MQ=255)                                                              gatGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTACCGGGtttt               >  1:1958951/3‑62 (MQ=255)                                                               ttGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCg                                 >  1:2265872/1‑43 (MQ=255)                                                                 gAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttttt                        >  1:1997915/1‑50 (MQ=255)                                                                 gAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTc     >  1:2861031/1‑69 (MQ=255)                                                                 gAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCg    <  1:2574956/70‑1 (MQ=255)                                                                  aaaCGTACTGCGTAGCCAGATACACGGATGGTCAGCt                                    <  1:248729/37‑1 (MQ=255)                                                                  aaaCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCCTCGag  <  1:589931/71‑1 (MQ=255)                                                                   aaCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGat                              <  1:1339895/42‑1 (MQ=255)                                                                   aaCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCg    >  1:1936877/1‑68 (MQ=255)                                                                    aCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGag  >  1:529629/1‑69 (MQ=255)                                                                    aCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGag  >  1:566372/1‑69 (MQ=255)                                                                    |                                                                     ATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAG  >  minE/607970‑608105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑