Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,040	T→C	7.8%	V738V (GTA→GTG)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,040	0	T	C	7.8%	180.3 / 5.4	64	V738V (GTA→GTG)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base T (32/27);  new base C (2/3);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

TTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCA  >  minE/607971‑608107                                                                      |                                                                    ttACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTa                                                                    >  1:34797/1‑71 (MQ=255) ttACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTa                                                                    <  1:2432002/71‑1 (MQ=255)   aCATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTAc                                                                   <  1:1389128/70‑1 (MQ=255)    cATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTg                                                                 >  1:1887533/1‑71 (MQ=255)     aTAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGc                                                                >  1:3546040/1‑71 (MQ=255)      tAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCg                                                               <  1:616304/71‑1 (MQ=255)           tGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAga                                                           >  1:2313334/1‑69 (MQ=255)           tGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGc                                                           >  1:2898512/1‑70 (MQ=255)             aGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTTTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCa                                                         <  1:2450779/70‑1 (MQ=255)             aGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTTTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCa                                                         <  1:2455529/70‑1 (MQ=255)              gtaaaGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGa                                                       <  1:3531908/68‑1 (MQ=255)              gTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGcc                                                          <  1:3339913/68‑1 (MQ=255)               tGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGa                                                       >  1:3822705/1‑70 (MQ=255)                 aaGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATac                                                    <  1:2826648/71‑1 (MQ=255)                  aGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATac                                                    <  1:3332902/70‑1 (MQ=255)                    gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                                                 <  1:3737148/71‑1 (MQ=255)                    gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                                                 >  1:2028385/1‑71 (MQ=255)                    gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                                                 <  1:2535925/71‑1 (MQ=255)                     tACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACgg                                                <  1:3117734/71‑1 (MQ=255)                     tACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACgg                                                <  1:283522/71‑1 (MQ=255)                       cGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGa                                               <  1:827193/70‑1 (MQ=255)                       cGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGa                                               <  1:2488753/70‑1 (MQ=255)                        gAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGAt                                              >  1:591593/1‑70 (MQ=255)                          gaaataaCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGt                                           >  1:1494929/3‑71 (MQ=255)                          gTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                                           >  1:964003/1‑71 (MQ=255)                          gTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                                           >  1:580131/1‑71 (MQ=255)                           taataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                                           >  1:2108558/1‑70 (MQ=255)                            aataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATaca                                                   >  1:2941795/1‑61 (MQ=255)                             ataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAg                                        >  1:1282906/1‑71 (MQ=255)                             ataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAg                                        >  1:1002818/1‑71 (MQ=255)                              taaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCg                                                               <  1:3021267/47‑1 (MQ=255)                                aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt                                      <  1:2502981/70‑1 (MQ=255)                                aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt                                      <  1:264757/70‑1 (MQ=255)                                aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt                                      <  1:2694977/70‑1 (MQ=255)                                  gTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATaca                                                   >  1:2011968/1‑55 (MQ=255)                                    ccTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACg                                                 <  1:705950/55‑1 (MQ=255)                                    ccTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa                                 >  1:145123/1‑71 (MQ=255)                                     ctgctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa                                 <  1:910165/70‑1 (MQ=255)                                       gctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGa                                                       >  1:33633/1‑46 (MQ=255)                                         tgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttt                            >  1:1300684/1‑71 (MQ=255)                                         tgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttt                            >  1:3792193/1‑71 (MQ=255)                                           ctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATg                                             >  1:2330520/1‑52 (MQ=255)                                              ttCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc                        <  1:2531698/70‑1 (MQ=255)                                              ttCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc                        <  1:1404730/70‑1 (MQ=255)                                              ttCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc                        <  1:2198588/70‑1 (MQ=255)                                                   tAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                                           >  1:3532970/1‑46 (MQ=255)                                                   tAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGttt                  >  1:941510/1‑71 (MQ=255)                                                      tCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc                        <  1:2401492/62‑1 (MQ=255)                                                        aGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGAt                                              >  1:1210512/1‑38 (MQ=255)                                                        aGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGAt                                              >  1:2091158/1‑38 (MQ=255)                                                        aGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCAt             >  1:1828542/1‑71 (MQ=255)                                                          cGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTg                                     <  1:2570363/45‑1 (MQ=255)                                                             gatGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTACCGGGtttt                 >  1:1958951/3‑62 (MQ=255)                                                              ttGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCg                                   >  1:2265872/1‑43 (MQ=255)                                                                gAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttttt                          >  1:1997915/1‑50 (MQ=255)                                                                gAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTc       >  1:2861031/1‑69 (MQ=255)                                                                gAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCg      <  1:2574956/70‑1 (MQ=255)                                                                 aaaCGTACTGCGTAGCCAGATACACGGATGGTCAGCt                                      <  1:248729/37‑1 (MQ=255)                                                                 aaaCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCCTCGag    <  1:589931/71‑1 (MQ=255)                                                                  aaCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGat                                <  1:1339895/42‑1 (MQ=255)                                                                  aaCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCg      >  1:1936877/1‑68 (MQ=255)                                                                   aCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGag    >  1:529629/1‑69 (MQ=255)                                                                   aCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGag    >  1:566372/1‑69 (MQ=255)                                                                     gTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGagca  >  1:3640/1‑69 (MQ=255)                                                                      |                                                                    TTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCA  >  minE/607971‑608107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑