BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,043	T→G	8.1%	A737A (GCA→GCC)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,043	0	T	G	8.1%	152.0 / 8.3	62	A737A (GCA→GCC)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base T (32/25); new base G (2/3); total (34/28)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

CATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAT  >  minE/607974‑608111

cATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTg                                                                     >  1:1887533/1‑71 (MQ=255)

 aTAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGc                                                                    >  1:3546040/1‑71 (MQ=255)

  tAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCg                                                                   <  1:616304/71‑1 (MQ=255)

       tGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAga                                                               >  1:2313334/1‑69 (MQ=255)

       tGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGc                                                               >  1:2898512/1‑70 (MQ=255)

         aGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTTTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCa                                                             <  1:2450779/70‑1 (MQ=255)

         aGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTTTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCa                                                             <  1:2455529/70‑1 (MQ=255)

          gtaaaGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGa                                                           <  1:3531908/68‑1 (MQ=255)

          gTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGcc                                                              <  1:3339913/68‑1 (MQ=255)

           tGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGa                                                           >  1:3822705/1‑70 (MQ=255)

             aaGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATac                                                        <  1:2826648/71‑1 (MQ=255)

              aGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATac                                                        <  1:3332902/70‑1 (MQ=255)

                gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                                                     <  1:2535925/71‑1 (MQ=255)

                gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                                                     <  1:3737148/71‑1 (MQ=255)

                gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                                                     >  1:2028385/1‑71 (MQ=255)

                 tACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACgg                                                    <  1:283522/71‑1 (MQ=255)

                 tACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACgg                                                    <  1:3117734/71‑1 (MQ=255)

                   cGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGa                                                   <  1:2488753/70‑1 (MQ=255)

                   cGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGa                                                   <  1:827193/70‑1 (MQ=255)

                    gAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGAt                                                  >  1:591593/1‑70 (MQ=255)

                      gaaataaCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGt                                               >  1:1494929/3‑71 (MQ=255)

                      gTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                                               >  1:964003/1‑71 (MQ=255)

                      gTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                                               >  1:580131/1‑71 (MQ=255)

                       taataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                                               >  1:2108558/1‑70 (MQ=255)

                        aataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATaca                                                       >  1:2941795/1‑61 (MQ=255)

                         ataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAg                                            >  1:1282906/1‑71 (MQ=255)

                         ataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAg                                            >  1:1002818/1‑71 (MQ=255)

                          taaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCg                                                                   <  1:3021267/47‑1 (MQ=255)

                            aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt                                          <  1:2694977/70‑1 (MQ=255)

                            aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt                                          <  1:264757/70‑1 (MQ=255)

                            aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt                                          <  1:2502981/70‑1 (MQ=255)

                              gTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATaca                                                       >  1:2011968/1‑55 (MQ=255)

                                ccTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACg                                                     <  1:705950/55‑1 (MQ=255)

                                ccTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa                                     >  1:145123/1‑71 (MQ=255)

                                 ctgctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa                                     <  1:910165/70‑1 (MQ=255)

                                   gctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGa                                                           >  1:33633/1‑46 (MQ=255)

                                     tgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttt                                >  1:3792193/1‑71 (MQ=255)

                                     tgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttt                                >  1:1300684/1‑71 (MQ=255)

                                       ctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATg                                                 >  1:2330520/1‑52 (MQ=255)

                                          ttCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc                            <  1:2198588/70‑1 (MQ=255)

                                          ttCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc                            <  1:1404730/70‑1 (MQ=255)

                                          ttCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc                            <  1:2531698/70‑1 (MQ=255)

                                               tAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                                               >  1:3532970/1‑46 (MQ=255)

                                               tAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGttt                      >  1:941510/1‑71 (MQ=255)

                                                  tCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc                            <  1:2401492/62‑1 (MQ=255)

                                                    aGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGAt                                                  >  1:2091158/1‑38 (MQ=255)

                                                    aGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGAt                                                  >  1:1210512/1‑38 (MQ=255)

                                                    aGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCAt                 >  1:1828542/1‑71 (MQ=255)

                                                      cGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTg                                         <  1:2570363/45‑1 (MQ=255)

                                                         gatGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTACCGGGtttt                     >  1:1958951/3‑62 (MQ=255)

                                                          ttGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCg                                       >  1:2265872/1‑43 (MQ=255)

                                                            gAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttttt                              >  1:1997915/1‑50 (MQ=255)

                                                            gAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTc           >  1:2861031/1‑69 (MQ=255)

                                                            gAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCg          <  1:2574956/70‑1 (MQ=255)

                                                             aaaCGTACTGCGTAGCCAGATACACGGATGGTCAGCt                                          <  1:248729/37‑1 (MQ=255)

                                                             aaaCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCCTCGag        <  1:589931/71‑1 (MQ=255)

                                                              aaCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGat                                    <  1:1339895/42‑1 (MQ=255)

                                                              aaCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCg          >  1:1936877/1‑68 (MQ=255)

                                                               aCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGag        >  1:529629/1‑69 (MQ=255)

                                                               aCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGag        >  1:566372/1‑69 (MQ=255)

                                                                 gTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGagca      >  1:3640/1‑69 (MQ=255)

                                                                   aCTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAt  >  1:3378394/1‑71 (MQ=255)

CATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAT  >  minE/607974‑608111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`