| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 608,070 | C→G | 15.5% | Q728H (CAG→CAC) ‡ | pflB ← | pyruvate formate lyase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 608,070 | 0 | C | G | 15.5% | 152.3 / 17.3 | 58 | Q728H (CAG→CAC) ‡ | pflB | pyruvate formate lyase I |
| Reads supporting (aligned to +/- strand): ref base C (26/23); new base G (3/6); total (29/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01 | |||||||||||
ACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGT > minE/608002‑608140 | aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt < 1:2694977/70‑1 (MQ=255)aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt < 1:264757/70‑1 (MQ=255)aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt < 1:2502981/70‑1 (MQ=255) ccTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa > 1:145123/1‑71 (MQ=255) ctgctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa < 1:910165/70‑1 (MQ=255) tgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttt > 1:3792193/1‑71 (MQ=255) tgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttt > 1:1300684/1‑71 (MQ=255) ttCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc < 1:1404730/70‑1 (MQ=255) ttCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc < 1:2531698/70‑1 (MQ=255) ttCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc < 1:2198588/70‑1 (MQ=255) tAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGttt > 1:941510/1‑71 (MQ=255) tCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc < 1:2401492/62‑1 (MQ=255) aGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCAt > 1:1828542/1‑71 (MQ=255) cGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTg < 1:2570363/45‑1 (MQ=255) gatGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTACCGGGtttt > 1:1958951/3‑62 (MQ=255) ttGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCg > 1:2265872/1‑43 (MQ=255) gAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttttt > 1:1997915/1‑50 (MQ=255) gAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTc > 1:2861031/1‑69 (MQ=255) gAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCg < 1:2574956/70‑1 (MQ=255) aaaCGTACTGCGTAGCCAGATACACGGATGGTCAGCt < 1:248729/37‑1 (MQ=255) aaaCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCCTCGag < 1:589931/71‑1 (MQ=255) aaCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGat < 1:1339895/42‑1 (MQ=255) aaCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCg > 1:1936877/1‑68 (MQ=255) aCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGag > 1:566372/1‑69 (MQ=255) aCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGag > 1:529629/1‑69 (MQ=255) gTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGagca > 1:3640/1‑69 (MQ=255) aCTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAt > 1:3378394/1‑71 (MQ=255) cggCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAtt < 1:3645971/69‑1 (MQ=12) cggCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAtt < 1:1265563/69‑1 (MQ=12) gCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAttt > 1:2362056/1‑70 (MQ=255) cGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCa > 1:2963865/1‑71 (MQ=255) gCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGt < 1:3744660/71‑1 (MQ=255) gCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCgg > 1:75985/1‑39 (MQ=9) gCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCgg > 1:931519/1‑39 (MQ=9) gCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGtgc > 1:1021577/1‑41 (MQ=9) cAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCga < 1:2729242/68‑2 (MQ=9) agagACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCg < 1:263141/66‑1 (MQ=9) aGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGtt < 1:871208/39‑1 (MQ=255) aGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtca > 1:396873/1‑71 (MQ=255) aGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtca < 1:385533/71‑1 (MQ=255) taTACACGGATGGTCAGCTGCGGATATTTTTCCGGGttt > 1:454934/2‑39 (MQ=255) gagacacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATtcat < 1:803100/68‑1 (MQ=9) gATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAttt < 1:303918/61‑1 (MQ=255) aTACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcat > 1:3380607/1‑70 (MQ=255) acacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc > 1:1149639/1‑71 (MQ=255) cacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc < 1:251882/70‑1 (MQ=255) acGGATGGTCAGCTGCGGAAATTTTTCCGGGTTTTCCATCGCGTCGagcagc > 1:25087/1‑52 (MQ=255) ggATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGttg < 1:1812077/71‑2 (MQ=255) ggATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGtt > 1:2612834/1‑70 (MQ=255) gTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAttt < 1:731714/49‑1 (MQ=255) tCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCAc < 1:160098/51‑1 (MQ=255) tCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTc < 1:928150/71‑1 (MQ=255) cAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt > 1:254837/1‑69 (MQ=255) aGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAg > 1:3155460/1‑71 (MQ=255) aGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAg < 1:3241491/71‑1 (MQ=255) aGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAg > 1:1010417/1‑71 (MQ=255) gCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcatc > 1:2916952/1‑56 (MQ=255) gCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCa < 1:1912623/69‑1 (MQ=255) cTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGt < 1:3583132/71‑1 (MQ=255) cTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGt < 1:2538351/71‑1 (MQ=255) cTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGt > 1:40621/1‑71 (MQ=255) | ACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGT > minE/608002‑608140 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |