BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,130	A→G	11.1%	V708V (GTT→GTC)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,130	0	A	G	11.1%	268.6 / 20.6	99	V708V (GTT→GTC)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base A (46/42); new base G (2/9); total (48/51)
Fisher's exact test for biased strand distribution p-value = 5.23e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

Only 100 of 103 total aligned reads displayed.

TCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCA  >  minE/608066‑608197

 cAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt                                                                >  1:254837/1‑69 (MQ=255)

  aGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAg                                                             >  1:1010417/1‑71 (MQ=255)

  aGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAg                                                             >  1:3155460/1‑71 (MQ=255)

  aGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAg                                                             <  1:3241491/71‑1 (MQ=255)

   gCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCa                                                              <  1:1912623/69‑1 (MQ=255)

    cTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGt                                                           >  1:40621/1‑71 (MQ=255)

    cTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGt                                                           <  1:2538351/71‑1 (MQ=255)

    cTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGt                                                           <  1:3583132/71‑1 (MQ=255)

      gCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGc                                                         >  1:3690568/1‑71 (MQ=255)

         gATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGa                                                      <  1:1929460/71‑1 (MQ=255)

         gATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGa                                                      >  1:616967/1‑71 (MQ=12)

            atTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGacca                                                   >  1:564393/1‑71 (MQ=255)

             tttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGaccac                                                  <  1:2149654/71‑1 (MQ=255)

              ttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGaccacc                                                 >  1:3251582/1‑71 (MQ=255)

              ttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGaccacc                                                 >  1:855116/1‑71 (MQ=255)

              ttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGaccacc                                                 >  1:534188/1‑71 (MQ=255)

               tttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGaccgcc                                                 <  1:3278536/70‑1 (MQ=255)

                   cGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCga                                            <  1:1278827/71‑1 (MQ=255)

                   cGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCga                                            <  1:868636/71‑1 (MQ=255)

                    gggTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgat                                           <  1:1627687/71‑1 (MQ=255)

                      gTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg                                          >  1:1553947/1‑70 (MQ=255)

                       ttttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAg                                                             <  1:237535/50‑1 (MQ=255)

                       ttttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAg                                                             <  1:1953432/50‑1 (MQ=255)

                       ttttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatgga                                        >  1:1659397/1‑71 (MQ=255)

                         ttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatggat                                       >  1:1977977/1‑70 (MQ=255)

                          tCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGc                                     >  1:3689004/1‑71 (MQ=255)

                           ccATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTGAGGTGCTGACCACCTTCGATGGATGc                                     <  1:3419300/70‑1 (MQ=255)

                           ccATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGc                                     <  1:1213134/70‑1 (MQ=255)

                            cATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAgg                                                            <  1:904983/46‑1 (MQ=255)

                            cATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCtt                                   >  1:3171065/1‑71 (MQ=255)

                             aTCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCtt                                               >  1:1577473/1‑58 (MQ=255)

                             aTCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCtt                                               >  1:1568133/1‑58 (MQ=255)

                             aTCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTc                                  >  1:350886/1‑71 (MQ=255)

                              tCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCg                                 >  1:136118/1‑71 (MQ=255)

                               cgcgTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAg                                                             >  1:1356558/1‑42 (MQ=255)

                                gcgTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTg                                                          >  1:3904503/1‑44 (MQ=255)

                                gcgTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTg                                                          >  1:2517574/1‑44 (MQ=255)

                                gcgTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTg                                                          >  1:1872647/1‑44 (MQ=255)

                                  gTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGa                                                      >  1:3597212/1‑46 (MQ=255)

                                  gTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtggt                             >  1:2158204/1‑71 (MQ=255)

                                   tCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCga                                            <  1:2830291/55‑1 (MQ=255)

                                     gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCt                                                        >  1:3094586/1‑41 (MQ=255)

                                     gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTc                                  <  1:37952/63‑1 (MQ=255)

                                     gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGtggtgg                           >  1:1551415/1‑70 (MQ=255)

                                     gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGtggtgg                           <  1:1775066/70‑1 (MQ=255)

                                     gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGtggtgg                           <  1:1361846/70‑1 (MQ=255)

                                     gAGCAGCAATTCACGGTTCATCACGTTAACGTTCAg                                                             >  1:1227772/1‑36 (MQ=255)

                                     cagcagcATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGtggtgg                           <  1:3562883/69‑1 (MQ=255)

                                     cagcagcATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGa                          <  1:1099635/70‑1 (MQ=255)

                                     cagcagcATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGa                          <  1:1307701/70‑1 (MQ=255)

                                      agcagcATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGaccgcc                                                 <  1:1502184/47‑1 (MQ=255)

                                      agcagcATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGaa                         <  1:2845961/71‑1 (MQ=12)

                                      agcagcATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGa                                                      >  1:2861022/1‑42 (MQ=255)

                                       gcagcaTTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGaaa                        <  1:2209198/71‑2 (MQ=255)

                                        cagcaTTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGt                       <  1:410139/71‑1 (MQ=255)

                                          gcaTTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTaa                     >  1:686513/1‑71 (MQ=255)

                                          gcaTTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTaa                     >  1:690240/1‑71 (MQ=255)

                                            aTTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAcc                   <  1:3187811/71‑1 (MQ=255)

                                             tttCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtggt                             <  1:3859320/60‑1 (MQ=255)

                                             tttCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAcca                  >  1:653763/1‑71 (MQ=255)

                                              ttCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccat                 >  1:1077683/1‑71 (MQ=255)

                                               tCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatggat                                       <  1:2052940/48‑1 (MQ=255)

                                                cACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtggt                             >  1:923323/1‑57 (MQ=255)

                                                 aCGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatcc               <  1:597418/70‑1 (MQ=255)

                                                 aCGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatcc               <  1:1859894/70‑1 (MQ=255)

                                                  cGGTTCATCACGTTAACGTTCAGGTGCTTACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat             <  1:3467065/71‑1 (MQ=255)

                                                  cGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat             <  1:1082883/71‑1 (MQ=255)

                                                  cGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat             <  1:103763/71‑1 (MQ=255)

                                                    gTTCATCACGTTAACGTTCAGGTTCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa           <  1:1865490/71‑1 (MQ=255)

                                                    gTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat             >  1:2550594/1‑69 (MQ=255)

                                                    gTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa           <  1:1093349/71‑1 (MQ=255)

                                                    gTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa           <  1:3007658/71‑1 (MQ=255)

                                                    gTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa           <  1:743175/71‑1 (MQ=255)

                                                     ttCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa           <  1:400357/70‑1 (MQ=255)

                                                       catcaCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAc        >  1:2114380/1‑71 (MQ=255)

                                                       catcaCGTTAACGATCAGGTGCTGACCACCTACgatgga                                        >  1:2430576/1‑39 (MQ=37)

                                                        atcaCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAc        <  1:1054824/70‑1 (MQ=255)

                                                         ttacgttgacgttgAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGccg      <  1:3135964/69‑2 (MQ=255)

                                                         tcaCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAcca      <  1:3408674/71‑1 (MQ=255)

                                                          tacgttgacgttgAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGaa                         >  1:556602/2‑51 (MQ=255)

                                                          caCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAg                        >  1:3902437/1‑52 (MQ=255)

                                                           aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagc    >  1:21870/1‑71 (MQ=255)

                                                           aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagc    >  1:1065758/1‑71 (MQ=255)

                                                           aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagc    >  1:2987300/1‑71 (MQ=255)

                                                           aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccag     >  1:462972/1‑70 (MQ=255)

                                                           aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccag     <  1:1483649/70‑1 (MQ=255)

                                                            cGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGaa                         >  1:2506238/1‑49 (MQ=255)

                                                             gTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACccatccag             <  1:2263703/60‑2 (MQ=255)

                                                             gTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACccatccag             <  1:3062689/60‑2 (MQ=255)

                                                             gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca  <  1:866714/71‑1 (MQ=255)

                                                             gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca  <  1:10364/71‑1 (MQ=255)

                                                             gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca  <  1:1102502/71‑1 (MQ=255)

                                                             gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca  <  1:1246005/71‑1 (MQ=255)

                                                              ttAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGt                       <  1:2996603/49‑1 (MQ=255)

                                                              ttAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTaa                     >  1:1356578/1‑51 (MQ=255)

                                                               tAACGTTCAGGTGCTGACCACCTTCGTTGGATGCtt                                   <  1:2838288/36‑1 (MQ=255)

                                                               tAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAc                    >  1:448236/1‑51 (MQ=255)

                                                               tAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAc                    >  1:3099265/1‑51 (MQ=255)

                                                                aaCGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatcc               >  1:1893065/1‑55 (MQ=255)

                                                                aaCGTTCAGGGGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTa                      <  1:3781202/48‑1 (MQ=255)

TCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCA  >  minE/608066‑608197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`