| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 189,223 | C→T | 9.7% | I240I (ATC→ATT) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 189,223 | 0 | C | T | 9.7% | 175.5 / 7.7 | 62 | I240I (ATC→ATT) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (30/26); new base T (2/4); total (32/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01 | |||||||||||
CAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGATGTAGTGCCAGTCTGGCTG > minE/189154‑189289 | cAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCg < 1:3005263/71‑1 (MQ=255)cAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCg < 1:2743344/71‑1 (MQ=255) aCGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATc > 1:3720346/1‑68 (MQ=255) aCGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATc > 1:820214/1‑68 (MQ=255) cGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGt > 1:930947/1‑71 (MQ=255) cGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGt > 1:6910/1‑71 (MQ=255) cGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGt > 1:233731/1‑71 (MQ=255) aCGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCa > 1:1704588/1‑71 (MQ=255) ctgcGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTaa < 1:248430/61‑1 (MQ=255) cGTCGAACAAAATCGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGa > 1:1360648/1‑58 (MQ=255) gTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCaa < 1:3052458/63‑1 (MQ=255) tCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATaa < 1:3226810/71‑1 (MQ=255) gcGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTaa > 1:3658149/2‑62 (MQ=255) cGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCACaaa > 1:1595306/1‑71 (MQ=255) cGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCACaa < 1:1529292/70‑1 (MQ=255) cGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCACaa < 1:958532/70‑1 (MQ=255) gAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCACAAAt < 1:1642960/71‑1 (MQ=255) gAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATaaa < 1:3724611/70‑1 (MQ=255) gAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAAt > 1:3053044/1‑71 (MQ=255) aaCAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATc < 1:3667322/71‑1 (MQ=255) aaCAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATc < 1:3354540/71‑1 (MQ=255) aaCAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATc > 1:632539/1‑71 (MQ=255) aCAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCg > 1:1985098/1‑71 (MQ=255) aCAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCg > 1:2615817/1‑71 (MQ=255) cAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGc > 1:1673698/1‑71 (MQ=255) aaaTTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTg > 1:3691979/1‑71 (MQ=255) aTTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAAATGTCATAAATCGCTgg < 1:845810/70‑1 (MQ=255) tgtgGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGgcg > 1:1209976/1‑70 (MQ=255) gtgGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCa > 1:146827/1‑71 (MQ=255) ggTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCt < 1:1883741/71‑1 (MQ=255) ggTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCt < 1:2441252/71‑1 (MQ=255) gTATGTACGCCGCGCCATCCGGGAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCt < 1:3763025/70‑1 (MQ=255) gTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCaa > 1:715104/1‑46 (MQ=255) aTGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAAt < 1:3402489/45‑1 (MQ=255) aTGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAAt < 1:1532994/45‑1 (MQ=255) aTGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGtt > 1:3883740/1‑71 (MQ=255) tGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGtt > 1:224687/1‑70 (MQ=255) tGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGtt < 1:213731/70‑1 (MQ=255) tGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGtt < 1:2850883/70‑1 (MQ=255) tACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTtgat > 1:3370770/1‑71 (MQ=255) cgccgcGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTtgatga > 1:3658826/1‑71 (MQ=255) cgccgcGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTtgatg < 1:2470767/70‑1 (MQ=255) ccgcGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATa < 1:771326/45‑1 (MQ=255) ccgcGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATc < 1:3501113/49‑1 (MQ=255) cgcgCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTtgatgatg > 1:2218557/1‑70 (MQ=255) gcgcCATCCGGCAGTACGCTGTTGATCGCCCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGaa > 1:3147988/1‑71 (MQ=255) cgcCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAAc < 1:1653186/71‑1 (MQ=255) gcCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAAc > 1:581932/1‑70 (MQ=255) gcCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACg < 1:1890757/71‑1 (MQ=255) cATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGAt > 1:3187404/1‑71 (MQ=255) aTCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGATg > 1:1249521/1‑71 (MQ=255) gTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGATGTAGTGc > 1:3388078/1‑69 (MQ=255) tACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGATGt < 1:2320742/63‑1 (MQ=255) aCGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGATGTAGTGCCAGt > 1:3793682/1‑71 (MQ=255) cTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGATGTAGTGCCAGTCTg > 1:580036/1‑71 (MQ=255) cTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGATGTAGTGCCAGTCTg < 1:3839943/71‑1 (MQ=255) cTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGATGTAGTGCCAGTCTg > 1:932712/1‑71 (MQ=255) tGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAGCGATGTAGTGCCAGTCTgg > 1:2772591/1‑71 (MQ=255) ttGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGATGTAGTGCCAGTCTGGCt > 1:1939688/1‑71 (MQ=255) gATCGACCGCAATTGTCATAAATCGCTGGCGCATGTGTTGATGATGAACGATGTAGTGCCAGTCTgg < 1:993076/67‑1 (MQ=255) gATCGACCGCAATTGTCATAAATCGCTAGCGCATCTGTTGATGATGAACGATGTAGTGCCAGTCTGGCTg < 1:2872020/70‑1 (MQ=255) aTCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTtgatgat < 1:1157282/44‑1 (MQ=255) aTCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTtga > 1:2128045/1‑40 (MQ=255) tCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGATGTAGTGCCa < 1:853513/59‑1 (MQ=255) | CAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGATGATGAACGATGTAGTGCCAGTCTGGCTG > minE/189154‑189289 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |