BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,156	T→C	16.4%	I700V (ATC→GTC)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,156	0	T	C	16.4%	273.1 / 39.7	116	I700V (ATC→GTC)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base T (42/55); new base C (2/17); total (44/72)
Fisher's exact test for biased strand distribution p-value = 8.54e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

GGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTT  >  minE/608086‑608224

gggTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgat                                                                      <  1:1627687/71‑1 (MQ=255)

  gTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg                                                                     >  1:1553947/1‑70 (MQ=255)

     ttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatggat                                                                  >  1:1977977/1‑70 (MQ=255)

      tCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGc                                                                >  1:3689004/1‑71 (MQ=255)

       ccATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTGAGGTGCTGACCACCTTCGATGGATGc                                                                <  1:3419300/70‑1 (MQ=255)

        cATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCtt                                                              >  1:3171065/1‑71 (MQ=255)

         aTCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTc                                                             >  1:350886/1‑71 (MQ=255)

                 gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGtggtgg                                                      <  1:1775066/70‑1 (MQ=255)

                 gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGtggtgg                                                      >  1:1551415/1‑70 (MQ=255)

                 gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGtggtgg                                                      <  1:1361846/70‑1 (MQ=255)

                 cagcagcATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGtggtgg                                                      <  1:3562883/69‑1 (MQ=255)

                 cagcagcATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGa                                                     <  1:1099635/70‑1 (MQ=255)

                 cagcagcATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGa                                                     <  1:1307701/70‑1 (MQ=255)

                   gcagcaTTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGaaa                                                   <  1:2209198/71‑2 (MQ=255)

                    cagcaTTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGt                                                  <  1:410139/71‑1 (MQ=255)

                      gcaTTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTaa                                                >  1:690240/1‑71 (MQ=255)

                      gcaTTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTaa                                                >  1:686513/1‑71 (MQ=255)

                        aTTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAcc                                              <  1:3187811/71‑1 (MQ=255)

                         tttCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtggt                                                        <  1:3859320/60‑1 (MQ=255)

                          ttCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccat                                            >  1:1077683/1‑71 (MQ=255)

                           tCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatggat                                                                  <  1:2052940/48‑1 (MQ=255)

                            cACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtggt                                                        >  1:923323/1‑57 (MQ=255)

                             aCGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatcc                                          <  1:597418/70‑1 (MQ=255)

                             aCGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatcc                                          <  1:1859894/70‑1 (MQ=255)

                              cGGTTCATCACGTTAACGTTCAGGTGCTTACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat                                        <  1:3467065/71‑1 (MQ=255)

                              cGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat                                        <  1:103763/71‑1 (MQ=255)

                                gTTCATCACGTTAACGTTCAGGTTCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                                      <  1:1865490/71‑1 (MQ=255)

                                gTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat                                        >  1:2550594/1‑69 (MQ=255)

                                gTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                                      <  1:743175/71‑1 (MQ=255)

                                gTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                                      <  1:1093349/71‑1 (MQ=255)

                                gTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                                      <  1:3007658/71‑1 (MQ=255)

                                 ttCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                                      <  1:400357/70‑1 (MQ=255)

                                   catcaCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAc                                   >  1:2114380/1‑71 (MQ=255)

                                   catcaCGTTAACGATCAGGTGCTGACCACCTACgatgga                                                                   >  1:2430576/1‑39 (MQ=37)

                                    atcaCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAc                                   <  1:1054824/70‑1 (MQ=255)

                                     ttacgttgacgttgAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGccg                                 <  1:3135964/69‑2 (MQ=255)

                                     tcaCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAcca                                 <  1:3408674/71‑1 (MQ=255)

                                      tacgttgacgttgAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGaa                                                    >  1:556602/2‑51 (MQ=255)

                                       aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagc                               >  1:2987300/1‑71 (MQ=255)

                                       aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagc                               >  1:21870/1‑71 (MQ=255)

                                       aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagc                               >  1:1065758/1‑71 (MQ=255)

                                       aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccag                                >  1:462972/1‑70 (MQ=255)

                                       aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccag                                <  1:1483649/70‑1 (MQ=255)

                                        cGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGaa                                                    >  1:2506238/1‑49 (MQ=255)

                                         gTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACccatccag                                        <  1:2263703/60‑2 (MQ=255)

                                         gTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACccatccag                                        <  1:3062689/60‑2 (MQ=255)

                                         gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca                             <  1:1102502/71‑1 (MQ=255)

                                         gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca                             <  1:1246005/71‑1 (MQ=255)

                                         gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca                             <  1:10364/71‑1 (MQ=255)

                                          ttAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTaa                                                >  1:1356578/1‑51 (MQ=255)

                                          ttAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAc                                   >  1:3383687/1‑64 (MQ=255)

                                           tAACGTTCAGGTGCTGACCACCTTCGTTGGATGCtt                                                              <  1:2838288/36‑1 (MQ=255)

                                           tAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAc                                               >  1:3099265/1‑51 (MQ=255)

                                            aaCGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatcc                                          >  1:1893065/1‑55 (MQ=255)

                                            aaCGTTCAGGGGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTa                                                 <  1:3781202/48‑1 (MQ=255)

                                             aCGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGtt                         >  1:1799271/1‑71 (MQ=255)

                                             aCGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGtt                         <  1:3703610/71‑1 (MQ=255)

                                              cGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTg                        <  1:668188/71‑1 (MQ=255)

                                              cGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTg                        <  1:2821012/71‑1 (MQ=255)

                                              cGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTg                        <  1:2482210/71‑1 (MQ=255)

                                               gTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGtt                         >  1:3239501/1‑69 (MQ=255)

                                                ttCAGGTGCTGACCACCTTCGATGGATGCTTCgtgg                                                         <  1:2320480/36‑1 (MQ=255)

                                                ttCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat                                        <  1:1175710/53‑1 (MQ=255)

                                                ttCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAc                                   <  1:3874000/58‑1 (MQ=255)

                                                 tCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTc                     >  1:1994647/1‑71 (MQ=255)

                                                  cAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTc                     <  1:3159160/70‑1 (MQ=255)

                                                  cAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTc                     >  1:1792913/1‑70 (MQ=255)

                                                  cAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                    >  1:401254/1‑71 (MQ=255)

                                                   aGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGtttt                   <  1:942347/71‑1 (MQ=255)

                                                   aGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGtttt                   <  1:2231230/71‑1 (MQ=255)

                                                   aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTaa                                                >  1:2491314/1‑42 (MQ=255)

                                                   aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                    <  1:2804426/70‑1 (MQ=255)

                                                   aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                    <  1:2343644/70‑1 (MQ=255)

                                                   aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                    <  1:2362760/70‑1 (MQ=255)

                                                   aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                    <  1:2689224/70‑1 (MQ=255)

                                                   aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAACCTGGCTGGTCt                    <  1:3119832/70‑1 (MQ=255)

                                                    ggTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTa                  <  1:3824925/71‑1 (MQ=255)

                                                    ggTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACAATCCATCAGACCAGCCAGGTTGGTCTTa                  <  1:3880655/71‑1 (MQ=255)

                                                     gtgtTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACccatcca                                         <  1:1868925/47‑1 (MQ=255)

                                                     gtgtTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACccatcca                                         <  1:2149349/47‑1 (MQ=255)

                                                     gTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                 <  1:1251432/71‑1 (MQ=255)

                                                     gTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                 >  1:923689/1‑71 (MQ=255)

                                                     gTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                 <  1:1614315/71‑1 (MQ=255)

                                                       gCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACg                >  1:2724014/1‑70 (MQ=255)

                                                           accaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACt            <  1:2984463/70‑1 (MQ=255)

                                                            ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                 >  1:2649502/1‑64 (MQ=255)

                                                            ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                 >  1:1479166/1‑64 (MQ=255)

                                                            ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc          >  1:2907297/1‑71 (MQ=255)

                                                             caccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtcg         >  1:2952013/1‑71 (MQ=255)

                                                               ccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtcgtc       <  1:924198/71‑1 (MQ=255)

                                                                  tCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCgtcgtc    >  1:3731260/1‑71 (MQ=255)

                                                                   cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAGGAACTTcgtcgt     <  1:2293151/69‑1 (MQ=255)

                                                                   cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTcgtcgt     <  1:474139/69‑1 (MQ=255)

                                                                   cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTcgtcgt     <  1:2823118/69‑1 (MQ=255)

                                                                   cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCgtcgtc    <  1:3724881/70‑1 (MQ=255)

                                                                   cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCgtcgtc    <  1:1797071/70‑1 (MQ=255)

                                                                   cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCt   >  1:3396910/1‑71 (MQ=255)

                                                                   cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCt   >  1:1495472/1‑71 (MQ=255)

                                                                    gatggatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCtt  >  1:3933899/1‑71 (MQ=255)

                                                                      tggatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                    <  1:2676588/51‑1 (MQ=255)

GGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTT  >  minE/608086‑608224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`