BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,158	G→T	16.3%	S699Y (TCC→TAC) ‡	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,158	0	G	T	16.3%	337.2 / 30.7	116	S699Y (TCC→TAC) ‡	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base G (42/55); new base T (2/17); total (44/72)
Fisher's exact test for biased strand distribution p-value = 8.54e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 123 total aligned reads displayed.

TTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTT  >  minE/608089‑608224

ttttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatgga                                                                   >  1:1659397/1‑71 (MQ=255)

  ttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatggat                                                                  >  1:1977977/1‑70 (MQ=255)

   tCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGc                                                                >  1:3689004/1‑71 (MQ=255)

    ccATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTGAGGTGCTGACCACCTTCGATGGATGc                                                                <  1:3419300/70‑1 (MQ=255)

     cATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCtt                                                              >  1:3171065/1‑71 (MQ=255)

      aTCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTc                                                             >  1:350886/1‑71 (MQ=255)

       tCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCg                                                            >  1:136118/1‑71 (MQ=255)

           gTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtggt                                                        >  1:2158204/1‑71 (MQ=255)

              gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTc                                                             <  1:37952/63‑1 (MQ=255)

              gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGtggtgg                                                      <  1:1775066/70‑1 (MQ=255)

              cagcagcATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGa                                                     <  1:1099635/70‑1 (MQ=255)

              cagcagcATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGa                                                     <  1:1307701/70‑1 (MQ=255)

               agcagcATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGaa                                                    <  1:2845961/71‑1 (MQ=12)

                gcagcaTTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGaaa                                                   <  1:2209198/71‑2 (MQ=255)

                   gcaTTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTaa                                                >  1:690240/1‑71 (MQ=255)

                   gcaTTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTaa                                                >  1:686513/1‑71 (MQ=255)

                      tttCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtggt                                                        <  1:3859320/60‑1 (MQ=255)

                       ttCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccat                                            >  1:1077683/1‑71 (MQ=255)

                        tCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatggat                                                                  <  1:2052940/48‑1 (MQ=255)

                         cACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtggt                                                        >  1:923323/1‑57 (MQ=255)

                          aCGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatcc                                          <  1:597418/70‑1 (MQ=255)

                          aCGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatcc                                          <  1:1859894/70‑1 (MQ=255)

                           cGGTTCATCACGTTAACGTTCAGGTGCTTACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat                                        <  1:3467065/71‑1 (MQ=255)

                           cGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat                                        <  1:103763/71‑1 (MQ=255)

                             gTTCATCACGTTAACGTTCAGGTTCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                                      <  1:1865490/71‑1 (MQ=255)

                             gTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat                                        >  1:2550594/1‑69 (MQ=255)

                             gTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                                      <  1:3007658/71‑1 (MQ=255)

                             gTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                                      <  1:743175/71‑1 (MQ=255)

                             gTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                                      <  1:1093349/71‑1 (MQ=255)

                              ttCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                                      <  1:400357/70‑1 (MQ=255)

                                catcaCGTTAACGATCAGGTGCTGACCACCTACgatgga                                                                   >  1:2430576/1‑39 (MQ=37)

                                 atcaCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAc                                   <  1:1054824/70‑1 (MQ=255)

                                  ttacgttgacgttgAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGccg                                 <  1:3135964/69‑2 (MQ=255)

                                   tacgttgacgttgAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGaa                                                    >  1:556602/2‑51 (MQ=255)

                                   caCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAg                                                   >  1:3902437/1‑52 (MQ=255)

                                    aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagc                               >  1:2987300/1‑71 (MQ=255)

                                    aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagc                               >  1:21870/1‑71 (MQ=255)

                                    aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagc                               >  1:1065758/1‑71 (MQ=255)

                                    aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccag                                >  1:462972/1‑70 (MQ=255)

                                    aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccag                                <  1:1483649/70‑1 (MQ=255)

                                     cGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGaa                                                    >  1:2506238/1‑49 (MQ=255)

                                      gTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACccatccag                                        <  1:3062689/60‑2 (MQ=255)

                                      gTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACccatccag                                        <  1:2263703/60‑2 (MQ=255)

                                      gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca                             <  1:10364/71‑1 (MQ=255)

                                      gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca                             <  1:1246005/71‑1 (MQ=255)

                                      gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca                             <  1:1102502/71‑1 (MQ=255)

                                       ttAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGt                                                  <  1:2996603/49‑1 (MQ=255)

                                       ttAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTaa                                                >  1:1356578/1‑51 (MQ=255)

                                       ttAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAc                                   >  1:3383687/1‑64 (MQ=255)

                                        tAACGTTCAGGTGCTGACCACCTTCGTTGGATGCtt                                                              <  1:2838288/36‑1 (MQ=255)

                                        tAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAc                                               >  1:448236/1‑51 (MQ=255)

                                          aCGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGtt                         >  1:1799271/1‑71 (MQ=255)

                                          aCGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGtt                         <  1:3703610/71‑1 (MQ=255)

                                           cGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTg                        <  1:668188/71‑1 (MQ=255)

                                           cGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTg                        <  1:3223304/71‑1 (MQ=255)

                                           cGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTg                        <  1:2821012/71‑1 (MQ=255)

                                           cGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTg                        <  1:2482210/71‑1 (MQ=255)

                                            gTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGtt                         >  1:3239501/1‑69 (MQ=255)

                                             ttCAGGTGCTGACCACCTTCGATGGATGCTTCgtgg                                                         <  1:2320480/36‑1 (MQ=255)

                                             ttCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat                                        <  1:1175710/53‑1 (MQ=255)

                                             ttCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAc                                   <  1:3874000/58‑1 (MQ=255)

                                              tCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTc                     >  1:1994647/1‑71 (MQ=255)

                                               gaGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGttt                    >  1:2508660/2‑69 (MQ=255)

                                               cAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTc                     <  1:3159160/70‑1 (MQ=255)

                                               cAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTc                     >  1:1792913/1‑70 (MQ=255)

                                               cAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                    >  1:401254/1‑71 (MQ=255)

                                               cAGGTGCTGACCACCTTCGATGGATGCTTCGTGGT‑GAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                    >  1:2402537/1‑70 (MQ=255)

                                                aGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGtttt                   <  1:942347/71‑1 (MQ=255)

                                                aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTaa                                                >  1:2491314/1‑42 (MQ=255)

                                                aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                    <  1:2343644/70‑1 (MQ=255)

                                                aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                    <  1:2804426/70‑1 (MQ=255)

                                                aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                    <  1:2689224/70‑1 (MQ=255)

                                                aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                    <  1:180736/70‑1 (MQ=255)

                                                aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                    <  1:2700611/70‑1 (MQ=255)

                                                aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAACCTGGCTGGTCt                    <  1:3119832/70‑1 (MQ=255)

                                                 ggTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTa                  <  1:3409193/71‑1 (MQ=255)

                                                 ggTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTa                  <  1:3824925/71‑1 (MQ=255)

                                                  gtgtTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACccatcca                                         <  1:2149349/47‑1 (MQ=255)

                                                  gtgtTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACccatcca                                         <  1:1868925/47‑1 (MQ=255)

                                                  gTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                 <  1:1614315/71‑1 (MQ=255)

                                                  gTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                 <  1:1993034/71‑1 (MQ=255)

                                                  gTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                 >  1:923689/1‑71 (MQ=255)

                                                  gTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                 <  1:1251432/71‑1 (MQ=255)

                                                    gCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACg                >  1:2724014/1‑70 (MQ=255)

                                                        accaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAAc             <  1:3631156/69‑1 (MQ=255)

                                                        accaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACt            >  1:1538295/1‑70 (MQ=255)

                                                         ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                 >  1:1479166/1‑64 (MQ=255)

                                                         ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                 >  1:2649502/1‑64 (MQ=255)

                                                         ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc          >  1:2907297/1‑71 (MQ=255)

                                                          caccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtcg         >  1:2952013/1‑71 (MQ=255)

                                                            ccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtcgtc       <  1:2284422/71‑1 (MQ=255)

                                                            ccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtcgtc       <  1:924198/71‑1 (MQ=255)

                                                               tCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCgtcgtc    >  1:3731260/1‑71 (MQ=255)

                                                                cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAGGAACTTcgtcgt     <  1:2293151/69‑1 (MQ=255)

                                                                cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTcgtcgt     <  1:474139/69‑1 (MQ=255)

                                                                cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCgtcgtc    <  1:3724881/70‑1 (MQ=255)

                                                                cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCt   >  1:3396910/1‑71 (MQ=255)

                                                                cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCt   >  1:1495472/1‑71 (MQ=255)

                                                                cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCt   >  1:3120132/1‑71 (MQ=255)

                                                                 gatggatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCtt  >  1:3933899/1‑71 (MQ=255)

TTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTT  >  minE/608089‑608224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`