BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,160	T→C	16.4%	A698A (GCA→GCG)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,160	0	T	C	16.4%	270.0 / 41.7	116	A698A (GCA→GCG)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base T (42/55); new base C (2/17); total (44/72)
Fisher's exact test for biased strand distribution p-value = 8.54e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

Only 100 of 122 total aligned reads displayed.

TTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCC  >  minE/608091‑608229

 tCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGc                                                                     >  1:3689004/1‑71 (MQ=255)

  ccATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTGAGGTGCTGACCACCTTCGATGGATGc                                                                     <  1:3419300/70‑1 (MQ=255)

   cATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCtt                                                                   >  1:3171065/1‑71 (MQ=255)

         gTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtggt                                                             >  1:2158204/1‑71 (MQ=255)

            gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTc                                                                  <  1:37952/63‑1 (MQ=255)

            gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGtggtgg                                                           <  1:1775066/70‑1 (MQ=255)

            gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGtggtgg                                                           <  1:1361846/70‑1 (MQ=255)

            cagcagcATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGtggtgg                                                           <  1:3562883/69‑1 (MQ=255)

            cagcagcATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGa                                                          <  1:1099635/70‑1 (MQ=255)

            cagcagcATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGa                                                          <  1:1307701/70‑1 (MQ=255)

             agcagcATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGaa                                                         <  1:2845961/71‑1 (MQ=12)

              gcagcaTTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGaaa                                                        <  1:2209198/71‑2 (MQ=255)

                 gcaTTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTaa                                                     >  1:686513/1‑71 (MQ=255)

                 gcaTTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTaa                                                     >  1:690240/1‑71 (MQ=255)

                   aTTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAcc                                                   <  1:3187811/71‑1 (MQ=255)

                    tttCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtggt                                                             <  1:3859320/60‑1 (MQ=255)

                    tttCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAcca                                                  >  1:653763/1‑71 (MQ=255)

                      tCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatggat                                                                       <  1:2052940/48‑1 (MQ=255)

                       cACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtggt                                                             >  1:923323/1‑57 (MQ=255)

                        aCGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatcc                                               <  1:1859894/70‑1 (MQ=255)

                         cGGTTCATCACGTTAACGTTCAGGTGCTTACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat                                             <  1:3467065/71‑1 (MQ=255)

                         cGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat                                             <  1:103763/71‑1 (MQ=255)

                         cGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat                                             <  1:1082883/71‑1 (MQ=255)

                           gTTCATCACGTTAACGTTCAGGTTCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                                           <  1:1865490/71‑1 (MQ=255)

                           gTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                                           <  1:743175/71‑1 (MQ=255)

                           gTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                                           <  1:3007658/71‑1 (MQ=255)

                           gTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                                           <  1:1093349/71‑1 (MQ=255)

                            ttCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                                           <  1:400357/70‑1 (MQ=255)

                              catcaCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAc                                        >  1:2114380/1‑71 (MQ=255)

                               atcaCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAc                                        <  1:1054824/70‑1 (MQ=255)

                                ttacgttgacgttgAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGccg                                      <  1:3135964/69‑2 (MQ=255)

                                tcaCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAcca                                      <  1:3408674/71‑1 (MQ=255)

                                 tacgttgacgttgAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGaa                                                         >  1:556602/2‑51 (MQ=255)

                                 caCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAg                                                        >  1:3902437/1‑52 (MQ=255)

                                  aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagc                                    >  1:21870/1‑71 (MQ=255)

                                  aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagc                                    >  1:2987300/1‑71 (MQ=255)

                                  aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagc                                    >  1:1065758/1‑71 (MQ=255)

                                  aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccag                                     <  1:1483649/70‑1 (MQ=255)

                                  aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccag                                     >  1:462972/1‑70 (MQ=255)

                                   cGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGaa                                                         >  1:2506238/1‑49 (MQ=255)

                                    gTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACccatccag                                             <  1:2263703/60‑2 (MQ=255)

                                    gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca                                  <  1:10364/71‑1 (MQ=255)

                                    gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca                                  <  1:1102502/71‑1 (MQ=255)

                                    gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca                                  <  1:1246005/71‑1 (MQ=255)

                                     ttAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGt                                                       <  1:2996603/49‑1 (MQ=255)

                                     ttAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTaa                                                     >  1:1356578/1‑51 (MQ=255)

                                     ttAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAc                                        >  1:3383687/1‑64 (MQ=255)

                                      tAACGTTCAGGTGCTGACCACCTTCGTTGGATGCtt                                                                   <  1:2838288/36‑1 (MQ=255)

                                      tAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAc                                                    >  1:448236/1‑51 (MQ=255)

                                      tAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAc                                                    >  1:3099265/1‑51 (MQ=255)

                                       aaCGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatcc                                               >  1:1893065/1‑55 (MQ=255)

                                       aaCGTTCAGGGGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTa                                                      <  1:3781202/48‑1 (MQ=255)

                                        aCGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGtt                              <  1:3703610/71‑1 (MQ=255)

                                         cGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTg                             <  1:2821012/71‑1 (MQ=255)

                                         cGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTg                             <  1:668188/71‑1 (MQ=255)

                                         cGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTg                             <  1:3223304/71‑1 (MQ=255)

                                         cGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTg                             <  1:2482210/71‑1 (MQ=255)

                                          gTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGtt                              >  1:3239501/1‑69 (MQ=255)

                                           ttCAGGTGCTGACCACCTTCGATGGATGCTTCgtgg                                                              <  1:2320480/36‑1 (MQ=255)

                                           ttCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat                                             <  1:1175710/53‑1 (MQ=255)

                                            tCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTc                          >  1:1994647/1‑71 (MQ=255)

                                             gaGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGttt                         >  1:2508660/2‑69 (MQ=255)

                                             cAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTc                          >  1:1792913/1‑70 (MQ=255)

                                             cAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                         >  1:401254/1‑71 (MQ=255)

                                              aGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGtttt                        <  1:942347/71‑1 (MQ=255)

                                              aGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGtttt                        <  1:2231230/71‑1 (MQ=255)

                                              aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTaa                                                     >  1:2491314/1‑42 (MQ=255)

                                              aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                         <  1:180736/70‑1 (MQ=255)

                                              aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                         <  1:2343644/70‑1 (MQ=255)

                                              aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                         <  1:2804426/70‑1 (MQ=255)

                                              aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                         <  1:2700611/70‑1 (MQ=255)

                                              aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                         <  1:2689224/70‑1 (MQ=255)

                                              aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                         <  1:2362760/70‑1 (MQ=255)

                                              aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAACCTGGCTGGTCt                         <  1:3119832/70‑1 (MQ=255)

                                               ggTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTa                       <  1:3409193/71‑1 (MQ=255)

                                               ggTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTa                       <  1:3824925/71‑1 (MQ=255)

                                               ggTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACAATCCATCAGACCAGCCAGGTTGGTCTTa                       <  1:3880655/71‑1 (MQ=255)

                                                gtgtTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACccatcca                                              <  1:1868925/47‑1 (MQ=255)

                                                gTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                      >  1:923689/1‑71 (MQ=255)

                                                gTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                      <  1:1251432/71‑1 (MQ=255)

                                                gTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                      <  1:1614315/71‑1 (MQ=255)

                                                gTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                      <  1:1993034/71‑1 (MQ=255)

                                                      accaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAAc                  <  1:3631156/69‑1 (MQ=255)

                                                      accaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACt                 >  1:1538295/1‑70 (MQ=255)

                                                      accaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACt                 <  1:2984463/70‑1 (MQ=255)

                                                       ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                      >  1:2649502/1‑64 (MQ=255)

                                                       ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                      >  1:1479166/1‑64 (MQ=255)

                                                        caccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtcg              >  1:2952013/1‑71 (MQ=255)

                                                          ccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtcgtc            <  1:924198/71‑1 (MQ=255)

                                                              cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAGGAACTTcgtcgt          <  1:2293151/69‑1 (MQ=255)

                                                              cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTcgtcgt          <  1:474139/69‑1 (MQ=255)

                                                              cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTcgtcgt          <  1:2823118/69‑1 (MQ=255)

                                                              cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCgtcgtc         <  1:1797071/70‑1 (MQ=255)

                                                              cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCgtcgtc         <  1:3724881/70‑1 (MQ=255)

                                                              cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCt        >  1:1495472/1‑71 (MQ=255)

                                                              cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCt        >  1:3120132/1‑71 (MQ=255)

                                                               gatggatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCtt       >  1:3933899/1‑71 (MQ=255)

                                                                 tggatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                         <  1:2676588/51‑1 (MQ=255)

                                                                   gatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                      >  1:2492549/1‑52 (MQ=255)

                                                                    atgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTAccc  <  1:1021903/71‑1 (MQ=255)

TTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCC  >  minE/608091‑608229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`