BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,178	A→C	14.3%	G692G (GGT→GGG)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,178	0	A	C	14.3%	223.6 / 41.9	112	G692G (GGT→GGG)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base A (41/55); new base C (3/13); total (44/68)
Fisher's exact test for biased strand distribution p-value = 9.72e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

Only 100 of 118 total aligned reads displayed.

GCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAAC  >  minE/608108‑608243

gcaTTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTaa                                                                   >  1:690240/1‑71 (MQ=255)

gcaTTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTaa                                                                   >  1:686513/1‑71 (MQ=255)

   tttCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAcca                                                                >  1:653763/1‑71 (MQ=255)

       aCGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatcc                                                             <  1:1859894/70‑1 (MQ=255)

       aCGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatcc                                                             <  1:597418/70‑1 (MQ=255)

        cGGTTCATCACGTTAACGTTCAGGTGCTTACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat                                                           <  1:3467065/71‑1 (MQ=255)

        cGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat                                                           <  1:103763/71‑1 (MQ=255)

        cGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat                                                           <  1:1082883/71‑1 (MQ=255)

          gTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat                                                           >  1:2550594/1‑69 (MQ=255)

          gTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                                                         <  1:743175/71‑1 (MQ=255)

          gTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                                                         <  1:1093349/71‑1 (MQ=255)

           ttCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                                                         <  1:400357/70‑1 (MQ=255)

              atcaCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAc                                                      <  1:1054824/70‑1 (MQ=255)

               ttacgttgacgttgAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGccg                                                    <  1:3135964/69‑2 (MQ=255)

               tcaCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAcca                                                    <  1:3408674/71‑1 (MQ=255)

                 aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagc                                                  >  1:1065758/1‑71 (MQ=255)

                 aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagc                                                  >  1:21870/1‑71 (MQ=255)

                 aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagc                                                  >  1:2987300/1‑71 (MQ=255)

                 aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccag                                                   >  1:462972/1‑70 (MQ=255)

                 aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccag                                                   <  1:1483649/70‑1 (MQ=255)

                   gTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACccatccag                                                           <  1:3062689/60‑2 (MQ=255)

                   gTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACccatccag                                                           <  1:2263703/60‑2 (MQ=255)

                   gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca                                                <  1:1246005/71‑1 (MQ=255)

                   gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca                                                <  1:1102502/71‑1 (MQ=255)

                   gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca                                                <  1:10364/71‑1 (MQ=255)

                   gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca                                                <  1:866714/71‑1 (MQ=255)

                     tAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAc                                                                  >  1:3099265/1‑51 (MQ=255)

                     tAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAc                                                                  >  1:448236/1‑51 (MQ=255)

                      aaCGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatcc                                                             >  1:1893065/1‑55 (MQ=255)

                       aCGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGtt                                            >  1:1799271/1‑71 (MQ=255)

                        cGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTg                                           <  1:2821012/71‑1 (MQ=255)

                        cGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTg                                           <  1:3223304/71‑1 (MQ=255)

                        cGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTg                                           <  1:668188/71‑1 (MQ=255)

                        cGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTg                                           <  1:2482210/71‑1 (MQ=255)

                         gTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGtt                                            >  1:3239501/1‑69 (MQ=255)

                          ttCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat                                                           <  1:1175710/53‑1 (MQ=255)

                          ttCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAc                                                      <  1:3874000/58‑1 (MQ=255)

                           tCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTc                                        >  1:1994647/1‑71 (MQ=255)

                            gaGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGttt                                       >  1:2508660/2‑69 (MQ=255)

                            cAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTc                                        <  1:3159160/70‑1 (MQ=255)

                            cAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTc                                        >  1:1792913/1‑70 (MQ=255)

                            cAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                       >  1:401254/1‑71 (MQ=255)

                            cAGGTGCTGACCACCTTCGATGGATGCTTCGTGGT‑GAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                       >  1:2402537/1‑70 (MQ=255)

                             aGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGtttt                                      <  1:942347/71‑1 (MQ=255)

                             aGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGtttt                                      <  1:2231230/71‑1 (MQ=255)

                             aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTaa                                                                   >  1:2491314/1‑42 (MQ=255)

                             aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                       <  1:2700611/70‑1 (MQ=255)

                             aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                       <  1:2343644/70‑1 (MQ=255)

                             aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                       <  1:2362760/70‑1 (MQ=255)

                             aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                       <  1:180736/70‑1 (MQ=255)

                             aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                       <  1:2804426/70‑1 (MQ=255)

                             aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAACCTGGCTGGTCt                                       <  1:3119832/70‑1 (MQ=255)

                              ggTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTa                                     <  1:3409193/71‑1 (MQ=255)

                              ggTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTa                                     <  1:3824925/71‑1 (MQ=255)

                               gtgtTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACccatcca                                                            <  1:2149349/47‑1 (MQ=255)

                               gtgtTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACccatcca                                                            <  1:1868925/47‑1 (MQ=255)

                               gTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                                    <  1:1251432/71‑1 (MQ=255)

                               gTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                                    >  1:923689/1‑71 (MQ=255)

                               gTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                                    <  1:1993034/71‑1 (MQ=255)

                                 gCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACg                                   >  1:2724014/1‑70 (MQ=255)

                                     accaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAAc                                <  1:3631156/69‑1 (MQ=255)

                                     accaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACt                               >  1:1538295/1‑70 (MQ=255)

                                     accaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACt                               <  1:2984463/70‑1 (MQ=255)

                                      ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                                    >  1:1479166/1‑64 (MQ=255)

                                      ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                                    >  1:2649502/1‑64 (MQ=255)

                                      ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                             >  1:2907297/1‑71 (MQ=255)

                                       caccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtcg                            >  1:2952013/1‑71 (MQ=255)

                                         ccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtcgtc                          <  1:2284422/71‑1 (MQ=255)

                                         ccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtcgtc                          <  1:924198/71‑1 (MQ=255)

                                            tCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCgtcgtc                       >  1:3731260/1‑71 (MQ=255)

                                             cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCgtcgtc                       <  1:1797071/70‑1 (MQ=255)

                                             cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCgtcgtc                       <  1:3724881/70‑1 (MQ=255)

                                             cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCt                      >  1:3396910/1‑71 (MQ=255)

                                             cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCt                      >  1:1495472/1‑71 (MQ=255)

                                             cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCt                      >  1:3120132/1‑71 (MQ=255)

                                              gatggatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCtt                     >  1:3933899/1‑71 (MQ=255)

                                                tggatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                       <  1:2676588/51‑1 (MQ=255)

                                                   atgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTAccc                <  1:1021903/71‑1 (MQ=255)

                                                      cTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTa                                     <  1:334105/47‑1 (MQ=255)

                                                      cTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTa                                     <  1:151775/47‑1 (MQ=255)

                                                      cTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTcgtcgt                        <  1:463558/60‑1 (MQ=255)

                                                      cTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGt             <  1:3467922/71‑1 (MQ=255)

                                                      cTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGt             <  1:2497312/71‑1 (MQ=255)

                                                      cTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAgc             <  1:504485/71‑2 (MQ=255)

                                                        tCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACtgg                             >  1:3358305/1‑51 (MQ=255)

                                                        tCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACtgg                             >  1:2273165/1‑51 (MQ=255)

                                                         cGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGaa                                 >  1:2059806/1‑48 (MQ=255)

                                                          gtggtgGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGt         >  1:1672782/1‑71 (MQ=255)

                                                             gtggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTc       <  1:546230/70‑1 (MQ=255)

                                                              tggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCgg     <  1:2630236/71‑1 (MQ=255)

                                                               ggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCtt                     <  1:2843820/54‑1 (MQ=255)

                                                               ggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCgg     >  1:677342/1‑70 (MQ=255)

                                                                 aaGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAAc  >  1:571959/1‑71 (MQ=255)

                                                                  aGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAAc  >  1:2924643/1‑70 (MQ=255)

                                                                    tAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                             >  1:3751140/1‑41 (MQ=255)

                                                                    tAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                             >  1:3342509/1‑41 (MQ=255)

                                                                    tAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTcgtcgt                        >  1:622982/1‑46 (MQ=255)

                                                                    tAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTAc                  >  1:1036054/1‑52 (MQ=255)

                                                                     aaCCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCgtcgtc                       >  1:856909/1‑46 (MQ=255)

                                                                     aaCCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCt                      >  1:2703262/1‑47 (MQ=255)

GCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAAC  >  minE/608108‑608243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`