BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,186	T→G	12.4%	M690L (ATG→CTG)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,186	0	T	G	12.4%	207.8 / 29.7	97	M690L (ATG→CTG)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base T (37/48); new base G (3/9); total (40/57)
Fisher's exact test for biased strand distribution p-value = 3.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 114 total aligned reads displayed.

CGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTG  >  minE/608116‑608256

cGGTTCATCACGTTAACGTTCAGGTGCTTACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat                                                                        <  1:3467065/71‑1 (MQ=255)

cGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat                                                                        <  1:103763/71‑1 (MQ=255)

cGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat                                                                        <  1:1082883/71‑1 (MQ=255)

  gTTCATCACGTTAACGTTCAGGTTCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                                                                      <  1:1865490/71‑1 (MQ=255)

  gTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat                                                                        >  1:2550594/1‑69 (MQ=255)

  gTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                                                                      <  1:3007658/71‑1 (MQ=255)

  gTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                                                                      <  1:743175/71‑1 (MQ=255)

   ttCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                                                                      <  1:400357/70‑1 (MQ=255)

     catcaCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAc                                                                   >  1:2114380/1‑71 (MQ=255)

      atcaCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAc                                                                   <  1:1054824/70‑1 (MQ=255)

       ttacgttgacgttgAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGccg                                                                 <  1:3135964/69‑2 (MQ=255)

       tcaCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAcca                                                                 <  1:3408674/71‑1 (MQ=255)

         aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagc                                                               >  1:21870/1‑71 (MQ=255)

         aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagc                                                               >  1:1065758/1‑71 (MQ=255)

         aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagc                                                               >  1:2987300/1‑71 (MQ=255)

         aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccag                                                                >  1:462972/1‑70 (MQ=255)

         aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccag                                                                <  1:1483649/70‑1 (MQ=255)

           gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca                                                             <  1:1246005/71‑1 (MQ=255)

           gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca                                                             <  1:1102502/71‑1 (MQ=255)

           gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca                                                             <  1:10364/71‑1 (MQ=255)

           gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca                                                             <  1:866714/71‑1 (MQ=255)

            ttAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAc                                                                   >  1:3383687/1‑64 (MQ=255)

               aCGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGtt                                                         <  1:3703610/71‑1 (MQ=255)

               aCGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGtt                                                         >  1:1799271/1‑71 (MQ=255)

                cGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTg                                                        <  1:668188/71‑1 (MQ=255)

                cGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTg                                                        <  1:2821012/71‑1 (MQ=255)

                cGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTg                                                        <  1:2482210/71‑1 (MQ=255)

                 gTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGtt                                                         >  1:3239501/1‑69 (MQ=255)

                  ttCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat                                                                        <  1:1175710/53‑1 (MQ=255)

                  ttCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAc                                                                   <  1:3874000/58‑1 (MQ=255)

                   tCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTc                                                     >  1:1994647/1‑71 (MQ=255)

                    cAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTc                                                     <  1:3159160/70‑1 (MQ=255)

                    cAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTc                                                     >  1:1792913/1‑70 (MQ=255)

                    cAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                                    >  1:401254/1‑71 (MQ=255)

                    cAGGTGCTGACCACCTTCGATGGATGCTTCGTGGT‑GAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                                    >  1:2402537/1‑70 (MQ=255)

                     aGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGtttt                                                   <  1:2231230/71‑1 (MQ=255)

                     aGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGtttt                                                   <  1:942347/71‑1 (MQ=255)

                     aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                                    <  1:2700611/70‑1 (MQ=255)

                     aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                                    <  1:2689224/70‑1 (MQ=255)

                     aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                                    <  1:2804426/70‑1 (MQ=255)

                     aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                                    <  1:2362760/70‑1 (MQ=255)

                     aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                                    <  1:180736/70‑1 (MQ=255)

                     aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                                    <  1:2343644/70‑1 (MQ=255)

                     aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAACCTGGCTGGTCt                                                    <  1:3119832/70‑1 (MQ=255)

                      ggTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACAATCCATCAGACCAGCCAGGTTGGTCTTa                                                  <  1:3880655/71‑1 (MQ=255)

                       gTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                                                 >  1:923689/1‑71 (MQ=255)

                       gTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                                                 <  1:1251432/71‑1 (MQ=255)

                             accaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAAc                                             <  1:3631156/69‑1 (MQ=255)

                             accaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACt                                            >  1:1538295/1‑70 (MQ=255)

                             accaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACt                                            <  1:2984463/70‑1 (MQ=255)

                              ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                                                 >  1:2649502/1‑64 (MQ=255)

                              ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                                                 >  1:1479166/1‑64 (MQ=255)

                              ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                                          >  1:2907297/1‑71 (MQ=255)

                                 ccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtcgtc                                       <  1:924198/71‑1 (MQ=255)

                                    tCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCgtcgtc                                    >  1:3731260/1‑71 (MQ=255)

                                     cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAGGAACTTcgtcgt                                     <  1:2293151/69‑1 (MQ=255)

                                     cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTcgtcgt                                     <  1:2823118/69‑1 (MQ=255)

                                     cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTcgtcgt                                     <  1:474139/69‑1 (MQ=255)

                                     cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCgtcgtc                                    <  1:1797071/70‑1 (MQ=255)

                                     cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCgtcgtc                                    <  1:3724881/70‑1 (MQ=255)

                                     cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCt                                   >  1:3120132/1‑71 (MQ=255)

                                     cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCt                                   >  1:3396910/1‑71 (MQ=255)

                                     cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCt                                   >  1:1495472/1‑71 (MQ=255)

                                      gatggatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCtt                                  >  1:3933899/1‑71 (MQ=255)

                                        tggatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                                    <  1:2676588/51‑1 (MQ=255)

                                          gatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                                                 >  1:2492549/1‑52 (MQ=255)

                                           atgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTAccc                             <  1:1021903/71‑1 (MQ=255)

                                              cTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTa                                                  <  1:334105/47‑1 (MQ=255)

                                              cTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTa                                                  <  1:151775/47‑1 (MQ=255)

                                              cTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGt                          <  1:2497312/71‑1 (MQ=255)

                                              cTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGt                          <  1:3467922/71‑1 (MQ=255)

                                              cTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAgc                          <  1:504485/71‑2 (MQ=255)

                                                tCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACtgg                                          >  1:3358305/1‑51 (MQ=255)

                                                tCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACtgg                                          >  1:2273165/1‑51 (MQ=255)

                                                 cGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGaa                                              >  1:2059806/1‑48 (MQ=255)

                                                  gtggtgGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGt                      >  1:1672782/1‑71 (MQ=255)

                                                   ttgtggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACt                                            <  1:824844/46‑1 (MQ=255)

                                                     gtggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTc                    <  1:546230/70‑1 (MQ=255)

                                                      tggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCgg                  <  1:2630236/71‑1 (MQ=255)

                                                       ggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCtt                                  <  1:2843820/54‑1 (MQ=255)

                                                        gAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTAccc                             >  1:637500/1‑58 (MQ=255)

                                                         aaGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAAc               >  1:571959/1‑71 (MQ=255)

                                                          aGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAAc               >  1:2924643/1‑70 (MQ=255)

                                                            tAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                                          >  1:3342509/1‑41 (MQ=255)

                                                            tAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                                          >  1:3751140/1‑41 (MQ=255)

                                                            tAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTcgtcgt                                     >  1:622982/1‑46 (MQ=255)

                                                            tAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTAc                               >  1:1036054/1‑52 (MQ=255)

                                                             aaCCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCgtcgtc                                    >  1:856909/1‑46 (MQ=255)

                                                             aaCCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCt                                   >  1:2703262/1‑47 (MQ=255)

                                                               ccatccatCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCg                       <  1:2832041/57‑1 (MQ=255)

                                                               ccatccatCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCg                       <  1:1975745/57‑1 (MQ=255)

                                                               ccatccatCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATa           >  1:1611263/1‑69 (MQ=255)

                                                                catccatcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATagag        <  1:1141675/71‑1 (MQ=255)

                                                                  tccatcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATagag        >  1:203586/1‑69 (MQ=255)

                                                                    catcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATa           >  1:3905764/1‑64 (MQ=255)

                                                                     atcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTg                         <  1:126335/49‑1 (MQ=255)

                                                                      tcAGACCAGCCAGGTTGGTCTTACGAACTtcgtcg                                      <  1:2829526/35‑1 (MQ=255)

                                                                      tcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAGCGATAGAGAAGgtg  >  1:1607805/1‑71 (MQ=255)

                                                                      tcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATa           <  1:1007353/62‑1 (MQ=255)

                                                                      tcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGgtg  <  1:1191281/71‑1 (MQ=255)

CGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTG  >  minE/608116‑608256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`