BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,190	A→G	11.1%	G688G (GGT→GGC)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,190	0	A	G	11.1%	291.4 / 22.9	108	G688G (GGT→GGC)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base A (44/52); new base G (3/9); total (47/61)
Fisher's exact test for biased strand distribution p-value = 2.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

Only 100 of 110 total aligned reads displayed.

CATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGG  >  minE/608121‑608260

 atcaCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAc                                                                       <  1:1054824/70‑1 (MQ=255)

  ttacgttgacgttgAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGccg                                                                     <  1:3135964/69‑2 (MQ=255)

  tcaCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAcca                                                                     <  1:3408674/71‑1 (MQ=255)

    aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagc                                                                   >  1:21870/1‑71 (MQ=255)

    aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagc                                                                   >  1:2987300/1‑71 (MQ=255)

    aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagc                                                                   >  1:1065758/1‑71 (MQ=255)

    aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccag                                                                    <  1:1483649/70‑1 (MQ=255)

    aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccag                                                                    >  1:462972/1‑70 (MQ=255)

      gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca                                                                 <  1:866714/71‑1 (MQ=255)

      gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca                                                                 <  1:1102502/71‑1 (MQ=255)

      gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca                                                                 <  1:1246005/71‑1 (MQ=255)

      gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca                                                                 <  1:10364/71‑1 (MQ=255)

          aCGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGtt                                                             <  1:3703610/71‑1 (MQ=255)

          aCGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGtt                                                             >  1:1799271/1‑71 (MQ=255)

           cGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTg                                                            <  1:668188/71‑1 (MQ=255)

           cGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTg                                                            <  1:2821012/71‑1 (MQ=255)

           cGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTg                                                            <  1:3223304/71‑1 (MQ=255)

            gTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGtt                                                             >  1:3239501/1‑69 (MQ=255)

              tCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTc                                                         >  1:1994647/1‑71 (MQ=255)

               gaGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGttt                                                        >  1:2508660/2‑69 (MQ=255)

               cAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTc                                                         <  1:3159160/70‑1 (MQ=255)

               cAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTc                                                         >  1:1792913/1‑70 (MQ=255)

               cAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                                        >  1:401254/1‑71 (MQ=255)

               cAGGTGCTGACCACCTTCGATGGATGCTTCGTGGT‑GAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                                        >  1:2402537/1‑70 (MQ=255)

                aGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGtttt                                                       <  1:2231230/71‑1 (MQ=255)

                aGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGtttt                                                       <  1:942347/71‑1 (MQ=255)

                aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                                        <  1:2689224/70‑1 (MQ=255)

                aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                                        <  1:2700611/70‑1 (MQ=255)

                aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                                        <  1:180736/70‑1 (MQ=255)

                aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                                        <  1:2362760/70‑1 (MQ=255)

                aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                                        <  1:2343644/70‑1 (MQ=255)

                aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAACCTGGCTGGTCt                                                        <  1:3119832/70‑1 (MQ=255)

                 ggTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTa                                                      <  1:3824925/71‑1 (MQ=255)

                 ggTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTa                                                      <  1:3409193/71‑1 (MQ=255)

                 ggTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACAATCCATCAGACCAGCCAGGTTGGTCTTa                                                      <  1:3880655/71‑1 (MQ=255)

                  gTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                                                     <  1:1993034/71‑1 (MQ=255)

                  gTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                                                     >  1:923689/1‑71 (MQ=255)

                    gCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACg                                                    >  1:2724014/1‑70 (MQ=255)

                        accaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAAc                                                 <  1:3631156/69‑1 (MQ=255)

                        accaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACt                                                >  1:1538295/1‑70 (MQ=255)

                        accaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACt                                                <  1:2984463/70‑1 (MQ=255)

                         ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                                                     >  1:1479166/1‑64 (MQ=255)

                         ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                                                     >  1:2649502/1‑64 (MQ=255)

                         ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                                              >  1:2907297/1‑71 (MQ=255)

                          caccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtcg                                             >  1:2952013/1‑71 (MQ=255)

                            ccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtcgtc                                           <  1:924198/71‑1 (MQ=255)

                            ccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtcgtc                                           <  1:2284422/71‑1 (MQ=255)

                               tCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCgtcgtc                                        >  1:3731260/1‑71 (MQ=255)

                                cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAGGAACTTcgtcgt                                         <  1:2293151/69‑1 (MQ=255)

                                cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTcgtcgt                                         <  1:474139/69‑1 (MQ=255)

                                cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTcgtcgt                                         <  1:2823118/69‑1 (MQ=255)

                                cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCgtcgtc                                        <  1:3724881/70‑1 (MQ=255)

                                cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCgtcgtc                                        <  1:1797071/70‑1 (MQ=255)

                                cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCt                                       >  1:3120132/1‑71 (MQ=255)

                                cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCt                                       >  1:1495472/1‑71 (MQ=255)

                                cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCt                                       >  1:3396910/1‑71 (MQ=255)

                                 gatggatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCtt                                      >  1:3933899/1‑71 (MQ=255)

                                   tggatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                                        <  1:2676588/51‑1 (MQ=255)

                                     gatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc                                                     >  1:2492549/1‑52 (MQ=255)

                                      atgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTAccc                                 <  1:1021903/71‑1 (MQ=255)

                                         cTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTa                                                      <  1:334105/47‑1 (MQ=255)

                                         cTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTa                                                      <  1:151775/47‑1 (MQ=255)

                                         cTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTcgtcgt                                         <  1:463558/60‑1 (MQ=255)

                                         cTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGt                              <  1:2497312/71‑1 (MQ=255)

                                         cTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGt                              <  1:3467922/71‑1 (MQ=255)

                                         cTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAgc                              <  1:504485/71‑2 (MQ=255)

                                           tCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACtgg                                              >  1:3358305/1‑51 (MQ=255)

                                           tCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACtgg                                              >  1:2273165/1‑51 (MQ=255)

                                            cGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGaa                                                  >  1:2059806/1‑48 (MQ=255)

                                             gtggtgGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGt                          >  1:1672782/1‑71 (MQ=255)

                                              ttgtggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACt                                                <  1:824844/46‑1 (MQ=255)

                                                gtggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTc                        <  1:546230/70‑1 (MQ=255)

                                                 tggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCgg                      <  1:2630236/71‑1 (MQ=255)

                                                  ggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCtt                                      <  1:2843820/54‑1 (MQ=255)

                                                  ggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCgg                      >  1:677342/1‑70 (MQ=255)

                                                   gAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTAccc                                 >  1:637500/1‑58 (MQ=255)

                                                     aGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAAc                   >  1:2924643/1‑70 (MQ=255)

                                                       tAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                                              >  1:3342509/1‑41 (MQ=255)

                                                       tAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                                              >  1:3751140/1‑41 (MQ=255)

                                                       tAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTcgtcgt                                         >  1:622982/1‑46 (MQ=255)

                                                       tAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTAc                                   >  1:1036054/1‑52 (MQ=255)

                                                        aaCCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCgtcgtc                                        >  1:856909/1‑46 (MQ=255)

                                                        aaCCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCt                                       >  1:2703262/1‑47 (MQ=255)

                                                          ccatccatCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCg                           <  1:1975745/57‑1 (MQ=255)

                                                          ccatccatCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCg                           <  1:2832041/57‑1 (MQ=255)

                                                          ccatccatCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATa               >  1:1611263/1‑69 (MQ=255)

                                                          ccatccatCAGACCACCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCg                           <  1:1263972/57‑1 (MQ=255)

                                                           catccatcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATagag            <  1:1141675/71‑1 (MQ=255)

                                                             tccatcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATagag            >  1:203586/1‑69 (MQ=255)

                                                               catcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATa               >  1:3905764/1‑64 (MQ=255)

                                                                atcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTg                             <  1:126335/49‑1 (MQ=255)

                                                                 tcAGACCAGCCAGGTTGGTCTTACGAACTtcgtcg                                          <  1:2829526/35‑1 (MQ=255)

                                                                 tcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAGCGATAGAGAAGgtg      >  1:1607805/1‑71 (MQ=255)

                                                                 tcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATa               <  1:1007353/62‑1 (MQ=255)

                                                                 tcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGgtg      <  1:1191281/71‑1 (MQ=255)

                                                                  cAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTc                        >  1:2140048/1‑52 (MQ=255)

                                                                   aGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGgtgt     <  1:3074812/70‑1 (MQ=255)

                                                                   aGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGgtg      >  1:151327/1‑69 (MQ=255)

                                                                     aCCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAgg  <  1:435437/71‑1 (MQ=255)

                                                                     aCCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAgg  <  1:2461678/71‑1 (MQ=255)

CATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGG  >  minE/608121‑608260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`