Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,298	A→C	11.8%	V652V (GTT→GTG)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,298	0	A	C	11.8%	165.7 / 20.5	76	V652V (GTT→GTG)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base A (35/32);  new base C (3/6);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

AGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACG  >  minE/608230‑608368                                                                     |                                                                       aGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAAc                                                                      >  1:7101/1‑71 (MQ=255)  gTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACg                                                                     <  1:3339879/71‑1 (MQ=255)   tGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACgg                                                                    <  1:269442/71‑1 (MQ=255)   tGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACgg                                                                    <  1:1722830/71‑1 (MQ=255)     cGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACgg                                                                    >  1:3834838/1‑69 (MQ=255)     cGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACgg                                                                    >  1:3867587/1‑69 (MQ=255)      gTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAg                                                                 >  1:369184/1‑71 (MQ=255)        tCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGt                                                                >  1:361587/1‑70 (MQ=255)         cGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGt                                                                >  1:3140270/1‑69 (MQ=255)          ggAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCaa                                                                       >  1:1670850/1‑61 (MQ=255)          ggAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCag                                                             <  1:3333443/71‑1 (MQ=255)          ggAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCag                                                             <  1:694873/71‑1 (MQ=255)          ggAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCag                                                             <  1:2044591/71‑1 (MQ=255)           gAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCag                                                             <  1:3827985/70‑1 (MQ=255)            aaCGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCa                                                              >  1:1412528/1‑68 (MQ=255)            aaCGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCa                                                              >  1:1982606/1‑68 (MQ=255)            aaCGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCa                                                              >  1:3352267/1‑68 (MQ=255)             aCGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCaat                                                            >  1:2992609/1‑67 (MQ=255)             aCGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCagaga                                                          <  1:324297/71‑1 (MQ=255)             aCGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCaga                                                            >  1:3283272/1‑69 (MQ=255)             aCGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCaga                                                            >  1:3302705/1‑69 (MQ=255)                attgagaAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGc                                                       <  1:267180/68‑1 (MQ=255)                attgagaAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGc                                                       <  1:2742794/68‑1 (MQ=255)                aTAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGc                                                       <  1:2445083/71‑1 (MQ=255)                 tAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACgg                                                                    >  1:1607299/1‑57 (MQ=255)                 tAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGc                                                       >  1:428323/1‑70 (MQ=255)                  agagAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCagag                                                           >  1:2221826/1‑65 (MQ=255)                    agaAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGc                                                       <  1:2212037/67‑1 (MQ=255)                    agaAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACt                                                   >  1:2131970/1‑71 (MQ=255)                      aaGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGaa                                                                  >  1:523529/1‑54 (MQ=255)                      aaGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTg                                                  >  1:1123783/1‑70 (MQ=255)                          tgtACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGac                                                                  <  1:2837786/50‑2 (MQ=255)                           gtAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCtt                                            >  1:953217/1‑71 (MQ=255)                            tacgagaTCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCttt                                           <  1:1753908/68‑1 (MQ=255)                              ggAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTc                                          <  1:2401660/70‑1 (MQ=255)                                  aTACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTc                                     <  1:1654394/71‑1 (MQ=255)                                    aCCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGt                                                                <  1:2029132/42‑1 (MQ=255)                                    aCCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTc                                     >  1:1331851/1‑69 (MQ=255)                                      cATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGa                                 >  1:720036/1‑71 (MQ=255)                                       aTCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGc                                                       <  1:3942664/48‑1 (MQ=255)                                       aTCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACATTTCTGGTCACGAc                                >  1:3260619/1‑71 (MQ=255)                                        tCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGAc                                >  1:1051499/1‑70 (MQ=255)                                          tttAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGt                             >  1:1980820/1‑71 (MQ=255)                                            tAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGTACCTTTCTGGTCACGACCGTg                            >  1:87529/1‑70 (MQ=255)                                            tAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGAcc                               <  1:2037412/67‑1 (MQ=255)                                               cGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATc                        <  1:3250035/71‑1 (MQ=255)                                               cGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTAACGACCGTGc                           <  1:913851/68‑1 (MQ=255)                                               cGTAAGAAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATc                        <  1:3164314/71‑1 (MQ=255)                                                 tAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTc                                          <  1:3559758/51‑1 (MQ=255)                                                 tAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCgg                      >  1:1019022/1‑71 (MQ=255)                                                 tAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTAATg                                                  <  1:355408/43‑1 (MQ=255)                                                   aGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGc                                                 >  1:2251034/1‑42 (MQ=255)                                                   aGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCATCTTTCTGGTCACGACCGTGCATCGGGt                    <  1:3696793/71‑1 (MQ=255)                                                   aGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGt                    >  1:850934/1‑71 (MQ=255)                                                   aGCAAACGGCAGTTTAGCAACGGAAGGCAGCGCGGCTACTGCACCTTTCTGGTCACGACCGTGCATCggg                     <  1:3344432/70‑1 (MQ=255)                                                    gtgaaCGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGtt                   <  1:2517230/68‑1 (MQ=255)                                                    gCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGt                                      <  1:1104843/52‑1 (MQ=255)                                                    gCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCgg                      >  1:1086037/1‑68 (MQ=255)                                                    gCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGtt                   <  1:3646045/71‑1 (MQ=255)                                                       aaCGGCAGTTTAGCAACGGAAGTCAGAGAGGCTAc                                                    >  1:1135848/1‑35 (MQ=255)                                                       aaCGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCa                                                >  1:1794823/1‑39 (MQ=255)                                                       aaCGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATc                        <  1:1799456/63‑1 (MQ=255)                                                        aCGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCa               <  1:552843/71‑1 (MQ=255)                                                         cGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGcgc              >  1:2072388/1‑69 (MQ=255)                                                          ggCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCAcc             <  1:1770690/71‑1 (MQ=255)                                                             aGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCgg          <  1:539325/71‑1 (MQ=255)                                                             aGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCgg          <  1:1867519/71‑1 (MQ=255)                                                              gTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCCTGCATCGGGTTAGCGCCCggc         <  1:3263540/71‑2 (MQ=255)                                                               tttAGCATCGGAAGTCTGAGAGGCTACTGCACCTTTc                                          <  1:2397951/37‑1 (MQ=255)                                                               tttAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGAcc                               <  1:2470539/48‑1 (MQ=255)                                                               tttAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTc        >  1:3031956/1‑71 (MQ=255)                                                                 tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTcc       >  1:2954541/1‑70 (MQ=255)                                                                 tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTcc       >  1:3643701/1‑70 (MQ=255)                                                                 tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTcc       >  1:878034/1‑70 (MQ=255)                                                                 tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTc        >  1:2297856/1‑69 (MQ=255)                                                                  aGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCAcc             >  1:323299/1‑63 (MQ=255)                                                                   gCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGa     <  1:1527459/70‑1 (MQ=255)                                                                     aaCGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACg  <  1:2364158/71‑1 (MQ=255)                                                                     |                                                                       AGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACG  >  minE/608230‑608368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑