Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 608,304 A→C 11.1% T650T (ACT→ACG)  pflB ← pyruvate formate lyase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE608,3040AC11.1% 159.1 / 17.5 72T650T (ACT→ACG) pflBpyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base A (33/31);  new base C (3/5);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 7.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01

GTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGC  >  minE/608235‑608374
                                                                     |                                                                      
gTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAg                                                                       >  1:369184/1‑71 (MQ=255)
  tCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGt                                                                      >  1:361587/1‑70 (MQ=255)
   cGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGt                                                                      >  1:3140270/1‑69 (MQ=255)
    ggAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCag                                                                   <  1:694873/71‑1 (MQ=255)
    ggAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCag                                                                   <  1:2044591/71‑1 (MQ=255)
    ggAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCag                                                                   <  1:3333443/71‑1 (MQ=255)
     gAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCag                                                                   <  1:3827985/70‑1 (MQ=255)
      aaCGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCa                                                                    >  1:1412528/1‑68 (MQ=255)
      aaCGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCa                                                                    >  1:1982606/1‑68 (MQ=255)
      aaCGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCa                                                                    >  1:3352267/1‑68 (MQ=255)
       aCGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCaat                                                                  >  1:2992609/1‑67 (MQ=255)
       aCGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCagaga                                                                <  1:324297/71‑1 (MQ=255)
       aCGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCaga                                                                  >  1:3283272/1‑69 (MQ=255)
       aCGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCaga                                                                  >  1:3302705/1‑69 (MQ=255)
          attgagaAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGc                                                             <  1:267180/68‑1 (MQ=255)
          attgagaAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGc                                                             <  1:2742794/68‑1 (MQ=255)
          aTAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGc                                                             <  1:2445083/71‑1 (MQ=255)
           tAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGc                                                             >  1:428323/1‑70 (MQ=255)
            agagAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCagag                                                                 >  1:2221826/1‑65 (MQ=255)
              agaAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGc                                                             <  1:2212037/67‑1 (MQ=255)
              agaAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACt                                                         >  1:2131970/1‑71 (MQ=255)
                aaGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGaa                                                                        >  1:523529/1‑54 (MQ=255)
                aaGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTg                                                        >  1:1123783/1‑70 (MQ=255)
                     gtAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCtt                                                  >  1:953217/1‑71 (MQ=255)
                      tacgagaTCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCttt                                                 <  1:1753908/68‑1 (MQ=255)
                        ggAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTc                                                <  1:2401660/70‑1 (MQ=255)
                            aTACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTc                                           <  1:1654394/71‑1 (MQ=255)
                              aCCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGt                                                                      <  1:2029132/42‑1 (MQ=255)
                              aCCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTc                                           >  1:1331851/1‑69 (MQ=255)
                                cATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGa                                       >  1:720036/1‑71 (MQ=255)
                                 aTCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGc                                                             <  1:3942664/48‑1 (MQ=255)
                                 aTCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACATTTCTGGTCACGAc                                      >  1:3260619/1‑71 (MQ=255)
                                  tCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGAc                                      >  1:1051499/1‑70 (MQ=255)
                                    tttAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGt                                   >  1:1980820/1‑71 (MQ=255)
                                      tAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGTACCTTTCTGGTCACGACCGTg                                  >  1:87529/1‑70 (MQ=255)
                                      tAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGAcc                                     <  1:2037412/67‑1 (MQ=255)
                                         cGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATc                              <  1:3250035/71‑1 (MQ=255)
                                         cGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTAACGACCGTGc                                 <  1:913851/68‑1 (MQ=255)
                                         cGTAAGAAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATc                              <  1:3164314/71‑1 (MQ=255)
                                           tAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTc                                                <  1:3559758/51‑1 (MQ=255)
                                           tAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCgg                            >  1:1019022/1‑71 (MQ=255)
                                           tAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTAATg                                                        <  1:355408/43‑1 (MQ=255)
                                             aGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGc                                                       >  1:2251034/1‑42 (MQ=255)
                                             aGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCATCTTTCTGGTCACGACCGTGCATCGGGt                          <  1:3696793/71‑1 (MQ=255)
                                             aGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGt                          >  1:850934/1‑71 (MQ=255)
                                             aGCAAACGGCAGTTTAGCAACGGAAGGCAGCGCGGCTACTGCACCTTTCTGGTCACGACCGTGCATCggg                           <  1:3344432/70‑1 (MQ=255)
                                              gtgaaCGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGtt                         <  1:2517230/68‑1 (MQ=255)
                                              gCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGt                                            <  1:1104843/52‑1 (MQ=255)
                                              gCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCgg                            >  1:1086037/1‑68 (MQ=255)
                                              gCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGtt                         <  1:3646045/71‑1 (MQ=255)
                                                 aaCGGCAGTTTAGCAACGGAAGTCAGAGAGGCTAc                                                          >  1:1135848/1‑35 (MQ=255)
                                                 aaCGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCa                                                      >  1:1794823/1‑39 (MQ=255)
                                                 aaCGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATc                              <  1:1799456/63‑1 (MQ=255)
                                                  aCGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCa                     <  1:552843/71‑1 (MQ=255)
                                                   cGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGcgc                    >  1:2072388/1‑69 (MQ=255)
                                                    ggCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCAcc                   <  1:1770690/71‑1 (MQ=255)
                                                       aGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCgg                <  1:1867519/71‑1 (MQ=255)
                                                       aGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCgg                <  1:539325/71‑1 (MQ=255)
                                                        gTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCCTGCATCGGGTTAGCGCCCggc               <  1:3263540/71‑2 (MQ=255)
                                                         tttAGCATCGGAAGTCTGAGAGGCTACTGCACCTTTc                                                <  1:2397951/37‑1 (MQ=255)
                                                         tttAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGAcc                                     <  1:2470539/48‑1 (MQ=255)
                                                         tttAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTc              >  1:3031956/1‑71 (MQ=255)
                                                           tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTcc             >  1:3643701/1‑70 (MQ=255)
                                                           tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTcc             >  1:2954541/1‑70 (MQ=255)
                                                           tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTcc             >  1:878034/1‑70 (MQ=255)
                                                           tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTc              >  1:2297856/1‑69 (MQ=255)
                                                            aGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCAcc                   >  1:323299/1‑63 (MQ=255)
                                                             gCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGa           <  1:1527459/70‑1 (MQ=255)
                                                               aaCGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACg        <  1:2364158/71‑1 (MQ=255)
                                                                aCGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACgg       >  1:3186160/1‑71 (MQ=255)
                                                                aCGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACg        <  1:3366397/70‑1 (MQ=255)
                                                                 cGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgc      >  1:1922/1‑71 (MQ=255)
                                                                  ggAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgcg     >  1:3165444/1‑71 (MQ=255)
                                                                    aaGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGc                      <  1:1505466/52‑1 (MQ=255)
                                                                    aaGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGc                      <  1:751043/52‑1 (MQ=255)
                                                                     aGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgcgcgc  >  1:779293/1‑71 (MQ=255)
                                                                     |                                                                      
GTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGC  >  minE/608235‑608374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑