Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,310	A→T	11.8%	S648S (TCT→TCA)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,310	0	A	T	11.8%	151.8 / 12.6	60	S648S (TCT→TCA)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base A (25/27);  new base T (2/5);  total (27/33)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

ACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCAC  >  minE/608242‑608380                                                                     |                                                                       aCGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCagaga                                                                      <  1:324297/71‑1 (MQ=255) aCGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCaga                                                                        >  1:3302705/1‑69 (MQ=255) aCGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCaga                                                                        >  1:3283272/1‑69 (MQ=255)    attgagaAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGc                                                                   <  1:2742794/68‑1 (MQ=255)    attgagaAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGc                                                                   <  1:267180/68‑1 (MQ=255)    aTAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGc                                                                   <  1:2445083/71‑1 (MQ=255)     tAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGc                                                                   >  1:428323/1‑70 (MQ=255)      agagAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCagag                                                                       >  1:2221826/1‑65 (MQ=255)        agaAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGc                                                                   <  1:2212037/67‑1 (MQ=255)        agaAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACt                                                               >  1:2131970/1‑71 (MQ=255)          aaGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTg                                                              >  1:1123783/1‑70 (MQ=255)               gtAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCtt                                                        >  1:953217/1‑71 (MQ=255)                tacgagaTCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCttt                                                       <  1:1753908/68‑1 (MQ=255)                  ggAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTc                                                      <  1:2401660/70‑1 (MQ=255)                      aTACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTc                                                 <  1:1654394/71‑1 (MQ=255)                        aCCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTc                                                 >  1:1331851/1‑69 (MQ=255)                          cATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGa                                             >  1:720036/1‑71 (MQ=255)                           aTCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGc                                                                   <  1:3942664/48‑1 (MQ=255)                           aTCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACATTTCTGGTCACGAc                                            >  1:3260619/1‑71 (MQ=255)                            tCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGAc                                            >  1:1051499/1‑70 (MQ=255)                              tttAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGt                                         >  1:1980820/1‑71 (MQ=255)                                tAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGTACCTTTCTGGTCACGACCGTg                                        >  1:87529/1‑70 (MQ=255)                                tAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGAcc                                           <  1:2037412/67‑1 (MQ=255)                                   cGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATc                                    <  1:3250035/71‑1 (MQ=255)                                   cGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTAACGACCGTGc                                       <  1:913851/68‑1 (MQ=255)                                   cGTAAGAAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATc                                    <  1:3164314/71‑1 (MQ=255)                                     tAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTc                                                      <  1:3559758/51‑1 (MQ=255)                                     tAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCgg                                  >  1:1019022/1‑71 (MQ=255)                                     tAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTAATg                                                              <  1:355408/43‑1 (MQ=255)                                       aGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGc                                                             >  1:2251034/1‑42 (MQ=255)                                       aGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCATCTTTCTGGTCACGACCGTGCATCGGGt                                <  1:3696793/71‑1 (MQ=255)                                       aGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGt                                >  1:850934/1‑71 (MQ=255)                                       aGCAAACGGCAGTTTAGCAACGGAAGGCAGCGCGGCTACTGCACCTTTCTGGTCACGACCGTGCATCggg                                 <  1:3344432/70‑1 (MQ=255)                                        gtgaaCGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGtt                               <  1:2517230/68‑1 (MQ=255)                                        gCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGt                                                  <  1:1104843/52‑1 (MQ=255)                                        gCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCgg                                  >  1:1086037/1‑68 (MQ=255)                                        gCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGtt                               <  1:3646045/71‑1 (MQ=255)                                           aaCGGCAGTTTAGCAACGGAAGTCAGAGAGGCTAc                                                                >  1:1135848/1‑35 (MQ=255)                                           aaCGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCa                                                            >  1:1794823/1‑39 (MQ=255)                                           aaCGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATc                                    <  1:1799456/63‑1 (MQ=255)                                            aCGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCa                           <  1:552843/71‑1 (MQ=255)                                             cGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGcgc                          >  1:2072388/1‑69 (MQ=255)                                              ggCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCAcc                         <  1:1770690/71‑1 (MQ=255)                                                 aGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCgg                      <  1:1867519/71‑1 (MQ=255)                                                 aGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCgg                      <  1:539325/71‑1 (MQ=255)                                                  gTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCCTGCATCGGGTTAGCGCCCggc                     <  1:3263540/71‑2 (MQ=255)                                                   tttAGCATCGGAAGTCTGAGAGGCTACTGCACCTTTc                                                      <  1:2397951/37‑1 (MQ=255)                                                   tttAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGAcc                                           <  1:2470539/48‑1 (MQ=255)                                                   tttAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTc                    >  1:3031956/1‑71 (MQ=255)                                                     tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTcc                   >  1:878034/1‑70 (MQ=255)                                                     tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTcc                   >  1:2954541/1‑70 (MQ=255)                                                     tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTcc                   >  1:3643701/1‑70 (MQ=255)                                                     tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTc                    >  1:2297856/1‑69 (MQ=255)                                                      aGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCAcc                         >  1:323299/1‑63 (MQ=255)                                                       gCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGa                 <  1:1527459/70‑1 (MQ=255)                                                         aaCGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACg              <  1:2364158/71‑1 (MQ=255)                                                          aCGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACgg             >  1:3186160/1‑71 (MQ=255)                                                          aCGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACg              <  1:3366397/70‑1 (MQ=255)                                                           cGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgc            >  1:1922/1‑71 (MQ=255)                                                            ggAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgcg           >  1:3165444/1‑71 (MQ=255)                                                              aaGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGc                            <  1:1505466/52‑1 (MQ=255)                                                              aaGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGc                            <  1:751043/52‑1 (MQ=255)                                                               aGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgcgcgc        >  1:779293/1‑71 (MQ=255)                                                                gTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCg                       <  1:2161746/55‑1 (MQ=255)                                                                   agagagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGtt                               >  1:546803/1‑44 (MQ=255)                                                                   agagagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCa      <  1:1961746/69‑1 (MQ=255)                                                                   agagagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGc    <  1:2220336/71‑1 (MQ=255)                                                                     agagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac  <  1:2923959/71‑1 (MQ=255)                                                                     |                                                                       ACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCAC  >  minE/608242‑608380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑