Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 608,328 C→G 6.2% Q642H (CAG→CAC) ‡ pflB ← pyruvate formate lyase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE608,3280CG 6.2% 192.8 / 2.9 64Q642H (CAG→CAC) ‡pflBpyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base C (26/34);  new base G (2/2);  total (28/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

GGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGT  >  minE/608259‑608396
                                                                     |                                                                    
ggAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTc                                                                      <  1:2401660/70‑1 (MQ=255)
    aTACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTc                                                                 <  1:1654394/71‑1 (MQ=255)
      aCCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTc                                                                 >  1:1331851/1‑69 (MQ=255)
        cATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGa                                                             >  1:720036/1‑71 (MQ=255)
         aTCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACATTTCTGGTCACGAc                                                            >  1:3260619/1‑71 (MQ=255)
          tCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGAc                                                            >  1:1051499/1‑70 (MQ=255)
            tttAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGt                                                         >  1:1980820/1‑71 (MQ=255)
              tAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGTACCTTTCTGGTCACGACCGTg                                                        >  1:87529/1‑70 (MQ=255)
              tAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGAcc                                                           <  1:2037412/67‑1 (MQ=255)
                 cGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATc                                                    <  1:3250035/71‑1 (MQ=255)
                 cGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTAACGACCGTGc                                                       <  1:913851/68‑1 (MQ=255)
                 cGTAAGAAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATc                                                    <  1:3164314/71‑1 (MQ=255)
                   tAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTc                                                                      <  1:3559758/51‑1 (MQ=255)
                   tAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCgg                                                  >  1:1019022/1‑71 (MQ=255)
                     aGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCATCTTTCTGGTCACGACCGTGCATCGGGt                                                <  1:3696793/71‑1 (MQ=255)
                     aGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGt                                                >  1:850934/1‑71 (MQ=255)
                     aGCAAACGGCAGTTTAGCAACGGAAGGCAGCGCGGCTACTGCACCTTTCTGGTCACGACCGTGCATCggg                                                 <  1:3344432/70‑1 (MQ=255)
                      gtgaaCGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGtt                                               <  1:2517230/68‑1 (MQ=255)
                      gCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGt                                                                  <  1:1104843/52‑1 (MQ=255)
                      gCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCgg                                                  >  1:1086037/1‑68 (MQ=255)
                      gCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGtt                                               <  1:3646045/71‑1 (MQ=255)
                         aaCGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATc                                                    <  1:1799456/63‑1 (MQ=255)
                          aCGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCa                                           <  1:552843/71‑1 (MQ=255)
                           cGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGcgc                                          >  1:2072388/1‑69 (MQ=255)
                            ggCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCAcc                                         <  1:1770690/71‑1 (MQ=255)
                               aGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCgg                                      <  1:539325/71‑1 (MQ=255)
                               aGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCgg                                      <  1:1867519/71‑1 (MQ=255)
                                gTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCCTGCATCGGGTTAGCGCCCggc                                     <  1:3263540/71‑2 (MQ=255)
                                 tttAGCATCGGAAGTCTGAGAGGCTACTGCACCTTTc                                                                      <  1:2397951/37‑1 (MQ=255)
                                 tttAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGAcc                                                           <  1:2470539/48‑1 (MQ=255)
                                 tttAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTc                                    >  1:3031956/1‑71 (MQ=255)
                                   tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTcc                                   >  1:3643701/1‑70 (MQ=255)
                                   tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTcc                                   >  1:2954541/1‑70 (MQ=255)
                                   tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTcc                                   >  1:878034/1‑70 (MQ=255)
                                   tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTc                                    >  1:2297856/1‑69 (MQ=255)
                                    aGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCAcc                                         >  1:323299/1‑63 (MQ=255)
                                     gCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGa                                 <  1:1527459/70‑1 (MQ=255)
                                       aaCGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACg                              <  1:2364158/71‑1 (MQ=255)
                                        aCGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACgg                             >  1:3186160/1‑71 (MQ=255)
                                        aCGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACg                              <  1:3366397/70‑1 (MQ=255)
                                         cGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgc                            >  1:1922/1‑71 (MQ=255)
                                          ggAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgcg                           >  1:3165444/1‑71 (MQ=255)
                                            aaGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGc                                            <  1:1505466/52‑1 (MQ=255)
                                            aaGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGc                                            <  1:751043/52‑1 (MQ=255)
                                             aGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgcgcgc                        >  1:779293/1‑71 (MQ=255)
                                              gTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCg                                       <  1:2161746/55‑1 (MQ=255)
                                                 agagagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGtt                                               >  1:546803/1‑44 (MQ=255)
                                                 agagagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCa                      <  1:1961746/69‑1 (MQ=255)
                                                 agagagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGc                    <  1:2220336/71‑1 (MQ=255)
                                                   agagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                  <  1:2923959/71‑1 (MQ=255)
                                                    gagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGtt                                               <  1:506275/41‑1 (MQ=255)
                                                    gagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGaa                                <  1:3905117/56‑1 (MQ=255)
                                                    gagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                  >  1:1172352/1‑70 (MQ=255)
                                                    gagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                  >  1:696155/1‑70 (MQ=255)
                                                     agGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGt                                                <  1:2689728/39‑1 (MQ=255)
                                                      ggCTACTGCACCTTTCTGGACACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacgac               <  1:3200847/71‑1 (MQ=255)
                                                       gCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                  <  1:836964/67‑1 (MQ=255)
                                                         tACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACgacgac            >  1:3728350/1‑71 (MQ=255)
                                                           cTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCg                                  <  1:1299860/47‑1 (MQ=255)
                                                             gCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacga                >  1:2314731/1‑63 (MQ=255)
                                                               aCCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTg      <  1:1445341/71‑1 (MQ=255)
                                                                 cTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTggg    >  1:669612/1‑71 (MQ=255)
                                                                 cTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTggg    >  1:161795/1‑71 (MQ=255)
                                                                   ttCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGgt  >  1:1575891/1‑71 (MQ=255)
                                                                   ttCTGGTCACGACCGTGCATCGGGTTAGCACCCGGGCCGAACGGCGCGCCAGCacgac               <  1:3335825/58‑1 (MQ=255)
                                                                    tCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGTCGCGCCAGCACGACGACCGTCTgggg   <  1:3913036/69‑1 (MQ=255)
                                                                    tCTGGTCACGACCGCGCATCGGCTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTgggg   <  1:3622849/69‑1 (MQ=255)
                                                                     |                                                                    
GGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGT  >  minE/608259‑608396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑