Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,355	A→G	12.9%	G633G (GGT→GGC)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,355	0	A	G	12.9%	154.4 / 13.3	62	G633G (GGT→GGC)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base A (29/25);  new base G (3/5);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01

ACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAAC  >  minE/608285‑608423                                                                       |                                                                     aCGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCa                                                                      <  1:552843/71‑1 (MQ=255)   ggCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCAcc                                                                    <  1:1770690/71‑1 (MQ=255)      aGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCgg                                                                 <  1:539325/71‑1 (MQ=255)      aGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCgg                                                                 <  1:1867519/71‑1 (MQ=255)       gTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCCTGCATCGGGTTAGCGCCCggc                                                                <  1:3263540/71‑2 (MQ=255)        tttAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTc                                                               >  1:3031956/1‑71 (MQ=255)          tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTcc                                                              >  1:2954541/1‑70 (MQ=255)          tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTcc                                                              >  1:878034/1‑70 (MQ=255)          tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTcc                                                              >  1:3643701/1‑70 (MQ=255)          tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTc                                                               >  1:2297856/1‑69 (MQ=255)           aGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCAcc                                                                    >  1:323299/1‑63 (MQ=255)            gCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGa                                                            <  1:1527459/70‑1 (MQ=255)              aaCGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACg                                                         <  1:2364158/71‑1 (MQ=255)               aCGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACgg                                                        >  1:3186160/1‑71 (MQ=255)               aCGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACg                                                         <  1:3366397/70‑1 (MQ=255)                cGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgc                                                       >  1:1922/1‑71 (MQ=255)                 ggAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgcg                                                      >  1:3165444/1‑71 (MQ=255)                    aGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgcgcgc                                                   >  1:779293/1‑71 (MQ=255)                     gTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCg                                                                  <  1:2161746/55‑1 (MQ=255)                        agagagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCa                                                 <  1:1961746/69‑1 (MQ=255)                        agagagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGc                                               <  1:2220336/71‑1 (MQ=255)                          agagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                                             <  1:2923959/71‑1 (MQ=255)                           gagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGaa                                                           <  1:3905117/56‑1 (MQ=255)                           gagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                                             >  1:696155/1‑70 (MQ=255)                           gagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                                             >  1:1172352/1‑70 (MQ=255)                             ggCTACTGCACCTTTCTGGACACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacgac                                          <  1:3200847/71‑1 (MQ=255)                              gCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                                             <  1:836964/67‑1 (MQ=255)                                tACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACgacgac                                       >  1:3728350/1‑71 (MQ=255)                                  cTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCg                                                             <  1:1299860/47‑1 (MQ=255)                                    gCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacga                                           >  1:2314731/1‑63 (MQ=255)                                      aCCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTg                                 <  1:1445341/71‑1 (MQ=255)                                        cTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTggg                               >  1:161795/1‑71 (MQ=255)                                        cTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTggg                               >  1:669612/1‑71 (MQ=255)                                          ttCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGgt                             >  1:1575891/1‑71 (MQ=255)                                          ttCTGGTCACGACCGTGCATCGGGTTAGCACCCGGGCCGAACGGCGCGCCAGCacgac                                          <  1:3335825/58‑1 (MQ=255)                                           tgcggTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCgt                             <  1:3566356/67‑1 (MQ=255)                                           tCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGTCGCGCCAGCACGACGACCGTCTgggg                              <  1:3913036/69‑1 (MQ=255)                                           tCTGGTCACGACCGCGCATCGGCTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTgggg                              <  1:3622849/69‑1 (MQ=255)                                               gTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTa                         <  1:3237536/70‑1 (MQ=255)                                               gTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTa                         <  1:319054/70‑1 (MQ=255)                                               gTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTAc                        >  1:1344474/1‑71 (MQ=255)                                               gTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTAc                        <  1:802030/71‑1 (MQ=255)                                                tCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTa                         >  1:3712209/1‑69 (MQ=255)                                                  aCGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTAccgg                     >  1:2130713/1‑69 (MQ=255)                                                    gACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGtt                   >  1:2110747/1‑71 (MQ=255)                                                      ccGTGCATCGGGTTAGCACCCGGTCCGAACGgcgcgc                                                   >  1:271689/1‑37 (MQ=255)                                                      ccGTGCATCGGGTTAGCACCCGGTCCGAACGgcgcgc                                                   >  1:2313018/1‑37 (MQ=255)                                                        atGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCt               <  1:2165478/70‑1 (MQ=255)                                                         tGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCtt              <  1:2506416/71‑1 (MQ=255)                                                           cATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCtggc            <  1:536931/71‑4 (MQ=255)                                                           cATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTAc            >  1:186636/1‑71 (MQ=255)                                                            aTCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTa             >  1:1333899/1‑69 (MQ=255)                                                             tCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGgt                             >  1:1597242/1‑52 (MQ=255)                                                             tCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa          <  1:2027753/71‑1 (MQ=255)                                                             tCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa          <  1:1570096/71‑1 (MQ=255)                                                              cGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa          >  1:477167/1‑70 (MQ=255)                                                              cGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa          >  1:3414294/1‑70 (MQ=255)                                                                  ttAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTacac     >  1:1069453/1‑71 (MQ=255)                                                                  ttAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTAccc                      <  1:2251354/54‑1 (MQ=255)                                                                   tAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTggg                               >  1:2723754/1‑44 (MQ=255)                                                                   tAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATacac     >  1:3024232/1‑70 (MQ=255)                                                                     gCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAAc  >  1:3832078/1‑71 (MQ=255)                                                                     gCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAAc  <  1:3354176/71‑1 (MQ=255)                                                                       |                                                                     ACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAAC  >  minE/608285‑608423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑