Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,361	T→C	11.4%	G631G (GGA→GGG) ‡	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,361	0	T	C	11.4%	158.1 / 11.2	62	G631G (GGA→GGG) ‡	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base T (31/23);  new base C (3/4);  total (34/28)
Fisher's exact test for biased strand distribution p-value = 6.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

TTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAG  >  minE/608292‑608428                                                                      |                                                                    tttAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTc                                                                    >  1:3031956/1‑71 (MQ=255)   tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTcc                                                                   >  1:878034/1‑70 (MQ=255)   tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTcc                                                                   >  1:3643701/1‑70 (MQ=255)   tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTcc                                                                   >  1:2954541/1‑70 (MQ=255)   tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTc                                                                    >  1:2297856/1‑69 (MQ=255)     gCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGa                                                                 <  1:1527459/70‑1 (MQ=255)       aaCGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACg                                                              <  1:2364158/71‑1 (MQ=255)        aCGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACgg                                                             >  1:3186160/1‑71 (MQ=255)        aCGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACg                                                              <  1:3366397/70‑1 (MQ=255)         cGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgc                                                            >  1:1922/1‑71 (MQ=255)          ggAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgcg                                                           >  1:3165444/1‑71 (MQ=255)             aGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgcgcgc                                                        >  1:779293/1‑71 (MQ=255)                 agagagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCa                                                      <  1:1961746/69‑1 (MQ=255)                 agagagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGc                                                    <  1:2220336/71‑1 (MQ=255)                   agagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                                                  <  1:2923959/71‑1 (MQ=255)                    gagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGaa                                                                <  1:3905117/56‑1 (MQ=255)                    gagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                                                  >  1:1172352/1‑70 (MQ=255)                    gagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                                                  >  1:696155/1‑70 (MQ=255)                      ggCTACTGCACCTTTCTGGACACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacgac                                               <  1:3200847/71‑1 (MQ=255)                       gCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                                                  <  1:836964/67‑1 (MQ=255)                         tACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACgacgac                                            >  1:3728350/1‑71 (MQ=255)                           cTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCg                                                                  <  1:1299860/47‑1 (MQ=255)                             gCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacga                                                >  1:2314731/1‑63 (MQ=255)                               aCCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTg                                      <  1:1445341/71‑1 (MQ=255)                                 cTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTggg                                    >  1:669612/1‑71 (MQ=255)                                 cTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTggg                                    >  1:161795/1‑71 (MQ=255)                                   ttCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGgt                                  >  1:1575891/1‑71 (MQ=255)                                   ttCTGGTCACGACCGTGCATCGGGTTAGCACCCGGGCCGAACGGCGCGCCAGCacgac                                               <  1:3335825/58‑1 (MQ=255)                                    tgcggTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCgt                                  <  1:3566356/67‑1 (MQ=255)                                    tCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGTCGCGCCAGCACGACGACCGTCTgggg                                   <  1:3913036/69‑1 (MQ=255)                                    tCTGGTCACGACCGCGCATCGGCTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTgggg                                   <  1:3622849/69‑1 (MQ=255)                                        gTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTa                              <  1:319054/70‑1 (MQ=255)                                        gTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTa                              <  1:3237536/70‑1 (MQ=255)                                        gTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTAc                             >  1:1344474/1‑71 (MQ=255)                                        gTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTAc                             <  1:802030/71‑1 (MQ=255)                                         tCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTa                              >  1:3712209/1‑69 (MQ=255)                                           aCGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTAccgg                          >  1:2130713/1‑69 (MQ=255)                                             gACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGtt                        >  1:2110747/1‑71 (MQ=255)                                               ccGTGCATCGGGTTAGCACCCGGTCCGAACGgcgcgc                                                        >  1:271689/1‑37 (MQ=255)                                               ccGTGCATCGGGTTAGCACCCGGTCCGAACGgcgcgc                                                        >  1:2313018/1‑37 (MQ=255)                                                 atGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCt                    <  1:2165478/70‑1 (MQ=255)                                                  tGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCtt                   <  1:2506416/71‑1 (MQ=255)                                                    cATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCtggc                 <  1:536931/71‑4 (MQ=255)                                                    cATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTAc                 >  1:186636/1‑71 (MQ=255)                                                     aTCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTa                  >  1:1333899/1‑69 (MQ=255)                                                      tCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGgt                                  >  1:1597242/1‑52 (MQ=255)                                                      tCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa               <  1:1570096/71‑1 (MQ=255)                                                      tCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa               <  1:2027753/71‑1 (MQ=255)                                                       cGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa               >  1:477167/1‑70 (MQ=255)                                                       cGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa               >  1:3414294/1‑70 (MQ=255)                                                           ttAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTacac          >  1:1069453/1‑71 (MQ=255)                                                           ttAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTAccc                           <  1:2251354/54‑1 (MQ=255)                                                            tAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTggg                                    >  1:2723754/1‑44 (MQ=255)                                                            tAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATacac          >  1:3024232/1‑70 (MQ=255)                                                              gCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAAc       >  1:3832078/1‑71 (MQ=255)                                                              gCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAAc       <  1:3354176/71‑1 (MQ=255)                                                                 cccGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGtt    <  1:1391101/71‑1 (MQ=255)                                                                 cccGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGt     >  1:2929608/1‑70 (MQ=255)                                                                  ccGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTa                  <  1:2315160/56‑1 (MQ=255)                                                                  ccGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGt     >  1:3540482/1‑69 (MQ=255)                                                                     gTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAAc       <  1:435448/64‑1 (MQ=255)                                                                     gTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAg  >  1:3297967/1‑69 (MQ=255)                                                                      tCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACAATACACaa        >  1:2691225/1‑62 (MQ=255)                                                                      |                                                                    TTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAG  >  minE/608292‑608428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑