Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,370	C→T	15.8%	A628A (GCG→GCA) ‡	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,370	0	C	T	15.8%	136.9 / 16.3	57	A628A (GCG→GCA) ‡	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base C (26/22);  new base T (4/5);  total (30/27)
Fisher's exact test for biased strand distribution p-value = 7.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

CGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGT  >  minE/608300‑608438                                                                       |                                                                     cGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgc                                                                      >  1:1922/1‑71 (MQ=255)  ggAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgcg                                                                     >  1:3165444/1‑71 (MQ=255)     aGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgcgcgc                                                                  >  1:779293/1‑71 (MQ=255)         agagagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCa                                                                <  1:1961746/69‑1 (MQ=255)         agagagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGc                                                              <  1:2220336/71‑1 (MQ=255)           agagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                                                            <  1:2923959/71‑1 (MQ=255)            gagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                                                            >  1:1172352/1‑70 (MQ=255)            gagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                                                            >  1:696155/1‑70 (MQ=255)              ggCTACTGCACCTTTCTGGACACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacgac                                                         <  1:3200847/71‑1 (MQ=255)               gCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                                                            <  1:836964/67‑1 (MQ=255)                 tACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACgacgac                                                      >  1:3728350/1‑71 (MQ=255)                     gCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacga                                                          >  1:2314731/1‑63 (MQ=255)                       aCCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTg                                                <  1:1445341/71‑1 (MQ=255)                         cTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTggg                                              >  1:161795/1‑71 (MQ=255)                         cTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTggg                                              >  1:669612/1‑71 (MQ=255)                           ttCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGgt                                            >  1:1575891/1‑71 (MQ=255)                           ttCTGGTCACGACCGTGCATCGGGTTAGCACCCGGGCCGAACGGCGCGCCAGCacgac                                                         <  1:3335825/58‑1 (MQ=255)                            tgcggTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCgt                                            <  1:3566356/67‑1 (MQ=255)                            tCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGTCGCGCCAGCACGACGACCGTCTgggg                                             <  1:3913036/69‑1 (MQ=255)                            tCTGGTCACGACCGCGCATCGGCTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTgggg                                             <  1:3622849/69‑1 (MQ=255)                                gTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTa                                        <  1:319054/70‑1 (MQ=255)                                gTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTa                                        <  1:3237536/70‑1 (MQ=255)                                gTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTAc                                       >  1:1344474/1‑71 (MQ=255)                                gTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTAc                                       <  1:802030/71‑1 (MQ=255)                                 tCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTa                                        >  1:3712209/1‑69 (MQ=255)                                   aCGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTAccgg                                    >  1:2130713/1‑69 (MQ=255)                                     gACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGtt                                  >  1:2110747/1‑71 (MQ=255)                                       ccGTGCATCGGGTTAGCACCCGGTCCGAACGgcgcgc                                                                  >  1:2313018/1‑37 (MQ=255)                                       ccGTGCATCGGGTTAGCACCCGGTCCGAACGgcgcgc                                                                  >  1:271689/1‑37 (MQ=255)                                         atGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCt                              <  1:2165478/70‑1 (MQ=255)                                          tGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCtt                             <  1:2506416/71‑1 (MQ=255)                                            cATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCtggc                           <  1:536931/71‑4 (MQ=255)                                            cATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTAc                           >  1:186636/1‑71 (MQ=255)                                             aTCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTa                            >  1:1333899/1‑69 (MQ=255)                                              tCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGgt                                            >  1:1597242/1‑52 (MQ=255)                                              tCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa                         <  1:2027753/71‑1 (MQ=255)                                              tCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa                         <  1:1570096/71‑1 (MQ=255)                                               cGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa                         >  1:3414294/1‑70 (MQ=255)                                               cGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa                         >  1:477167/1‑70 (MQ=255)                                                   ttAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTacac                    >  1:1069453/1‑71 (MQ=255)                                                   ttAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTAccc                                     <  1:2251354/54‑1 (MQ=255)                                                    tAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTggg                                              >  1:2723754/1‑44 (MQ=255)                                                    tAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATacac                    >  1:3024232/1‑70 (MQ=255)                                                      gCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAAc                 >  1:3832078/1‑71 (MQ=255)                                                      gCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAAc                 <  1:3354176/71‑1 (MQ=255)                                                         cccGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGtt              <  1:1391101/71‑1 (MQ=255)                                                         cccGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGt               >  1:2929608/1‑70 (MQ=255)                                                          ccGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTa                            <  1:2315160/56‑1 (MQ=255)                                                          ccGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGt               >  1:3540482/1‑69 (MQ=255)                                                             gTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAAc                 <  1:435448/64‑1 (MQ=255)                                                             gTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAg            >  1:3297967/1‑69 (MQ=255)                                                              tCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACAATACACaa                  >  1:2691225/1‑62 (MQ=255)                                                                cGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACAGGTTTTCTGGCCGTACACCACGTTAGAAGt        <  1:1287419/70‑1 (MQ=255)                                                                cGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGt        >  1:1936727/1‑70 (MQ=255)                                                                 gAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGa      >  1:3005560/1‑71 (MQ=255)                                                                 gAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa                         <  1:303295/52‑1 (MQ=255)                                                                 gAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGa      <  1:2975766/71‑1 (MQ=255)                                                                   aCGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATg    >  1:1373502/1‑71 (MQ=255)                                                                   aCGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATg    >  1:2005067/1‑71 (MQ=255)                                                                   aCGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATg    >  1:2077016/1‑71 (MQ=255)                                                                    cGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATg    >  1:2907238/1‑70 (MQ=255)                                                                     ggCGCGCCAGCACGACGACCGTCTGGGGTCTTACCCGTTTTCTTACCATacac                    <  1:273582/53‑1 (MQ=255)                                                                       cgcgcCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGt  >  1:2267719/1‑69 (MQ=255)                                                                       cgcgcCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGt  >  1:3077494/1‑69 (MQ=255)                                                                       |                                                                     CGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGT  >  minE/608300‑608438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑