Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,376	A→G	14.0%	A626A (GCT→GCC)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,376	0	A	G	14.0%	155.4 / 16.8	64	A626A (GCT→GCC)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base A (31/24);  new base G (4/5);  total (35/29)
Fisher's exact test for biased strand distribution p-value = 7.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

AGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAA  >  minE/608308‑608443                                                                     |                                                                    agagagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCa                                                                     <  1:1961746/69‑1 (MQ=255) agagagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGc                                                                   <  1:2220336/71‑1 (MQ=255)   agagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                                                                 <  1:2923959/71‑1 (MQ=255)    gagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                                                                 >  1:1172352/1‑70 (MQ=255)    gagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                                                                 >  1:696155/1‑70 (MQ=255)      ggCTACTGCACCTTTCTGGACACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacgac                                                              <  1:3200847/71‑1 (MQ=255)       gCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                                                                 <  1:836964/67‑1 (MQ=255)         tACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACgacgac                                                           >  1:3728350/1‑71 (MQ=255)             gCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacga                                                               >  1:2314731/1‑63 (MQ=255)               aCCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTg                                                     <  1:1445341/71‑1 (MQ=255)                 cTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTggg                                                   >  1:161795/1‑71 (MQ=255)                 cTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTggg                                                   >  1:669612/1‑71 (MQ=255)                   ttCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGgt                                                 >  1:1575891/1‑71 (MQ=255)                   ttCTGGTCACGACCGTGCATCGGGTTAGCACCCGGGCCGAACGGCGCGCCAGCacgac                                                              <  1:3335825/58‑1 (MQ=255)                    tgcggTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCgt                                                 <  1:3566356/67‑1 (MQ=255)                    tCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGTCGCGCCAGCACGACGACCGTCTgggg                                                  <  1:3913036/69‑1 (MQ=255)                    tCTGGTCACGACCGCGCATCGGCTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTgggg                                                  <  1:3622849/69‑1 (MQ=255)                        gTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTa                                             <  1:319054/70‑1 (MQ=255)                        gTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTa                                             <  1:3237536/70‑1 (MQ=255)                        gTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTAc                                            >  1:1344474/1‑71 (MQ=255)                        gTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTAc                                            <  1:802030/71‑1 (MQ=255)                         tCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTa                                             >  1:3712209/1‑69 (MQ=255)                           aCGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTAccgg                                         >  1:2130713/1‑69 (MQ=255)                             gACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGtt                                       >  1:2110747/1‑71 (MQ=255)                                 atGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCt                                   <  1:2165478/70‑1 (MQ=255)                                  tGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCtt                                  <  1:2506416/71‑1 (MQ=255)                                    cATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCtggc                                <  1:536931/71‑4 (MQ=255)                                    cATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTAc                                >  1:186636/1‑71 (MQ=255)                                     aTCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTa                                 >  1:1333899/1‑69 (MQ=255)                                      tCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGgt                                                 >  1:1597242/1‑52 (MQ=255)                                      tCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa                              <  1:2027753/71‑1 (MQ=255)                                      tCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa                              <  1:1570096/71‑1 (MQ=255)                                       cGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa                              >  1:477167/1‑70 (MQ=255)                                       cGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa                              >  1:3414294/1‑70 (MQ=255)                                           ttAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTacac                         >  1:1069453/1‑71 (MQ=255)                                           ttAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTAccc                                          <  1:2251354/54‑1 (MQ=255)                                            tAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTggg                                                   >  1:2723754/1‑44 (MQ=255)                                            tAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATacac                         >  1:3024232/1‑70 (MQ=255)                                              gCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAAc                      >  1:3832078/1‑71 (MQ=255)                                              gCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAAc                      <  1:3354176/71‑1 (MQ=255)                                                 cccGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGtt                   <  1:1391101/71‑1 (MQ=255)                                                 cccGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGt                    >  1:2929608/1‑70 (MQ=255)                                                  ccGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTa                                 <  1:2315160/56‑1 (MQ=255)                                                  ccGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGt                    >  1:3540482/1‑69 (MQ=255)                                                     gTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAAc                      <  1:435448/64‑1 (MQ=255)                                                     gTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAg                 >  1:3297967/1‑69 (MQ=255)                                                      tCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACAATACACaa                       >  1:2691225/1‑62 (MQ=255)                                                        cGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACAGGTTTTCTGGCCGTACACCACGTTAGAAGt             <  1:1287419/70‑1 (MQ=255)                                                        cGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGt             >  1:1936727/1‑70 (MQ=255)                                                         gAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGa           >  1:3005560/1‑71 (MQ=255)                                                         gAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa                              <  1:303295/52‑1 (MQ=255)                                                         gAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGa           <  1:2975766/71‑1 (MQ=255)                                                           aCGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATg         >  1:1373502/1‑71 (MQ=255)                                                           aCGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATg         >  1:2077016/1‑71 (MQ=255)                                                           aCGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATg         >  1:2005067/1‑71 (MQ=255)                                                            cGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATg         >  1:2907238/1‑70 (MQ=255)                                                             ggCGCGCCAGCACGACGACCGTCTGGGGTCTTACCCGTTTTCTTACCATacac                         <  1:273582/53‑1 (MQ=255)                                                               cgcgcCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGt       >  1:2267719/1‑69 (MQ=255)                                                               cgcgcCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGt       >  1:3077494/1‑69 (MQ=255)                                                                gcgcCAGCACGACGACCGTCTGGGGTGTTACCCGttt                                      <  1:2517088/37‑1 (MQ=255)                                                                gcgcCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa                              >  1:1721901/1‑45 (MQ=255)                                                                  gcCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa                              >  1:1526192/1‑43 (MQ=255)                                                                  gcCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGaa  >  1:30672/1‑71 (MQ=255)                                                                  gcCAGCACGACGACCGTCTGGGGCGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGaa  <  1:1787674/71‑1 (MQ=255)                                                                   ccAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAg    <  1:3632670/68‑1 (MQ=255)                                                                     aGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGa   >  1:1684856/1‑67 (MQ=255)                                                                     |                                                                    AGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAA  >  minE/608308‑608443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑