Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,379	A→G	14.7%	R625R (CGT→CGC)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,379	0	A	G	14.7%	145.3 / 16.8	61	R625R (CGT→CGC)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base A (33/19);  new base G (4/5);  total (37/24)
Fisher's exact test for biased strand distribution p-value = 2.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

AGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACT  >  minE/608310‑608449                                                                      |                                                                       agagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                                                                       <  1:2923959/71‑1 (MQ=255)  gagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                                                                       >  1:1172352/1‑70 (MQ=255)  gagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                                                                       >  1:696155/1‑70 (MQ=255)    ggCTACTGCACCTTTCTGGACACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacgac                                                                    <  1:3200847/71‑1 (MQ=255)     gCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                                                                       <  1:836964/67‑1 (MQ=255)       tACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACgacgac                                                                 >  1:3728350/1‑71 (MQ=255)           gCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacga                                                                     >  1:2314731/1‑63 (MQ=255)             aCCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTg                                                           <  1:1445341/71‑1 (MQ=255)               cTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTggg                                                         >  1:669612/1‑71 (MQ=255)               cTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTggg                                                         >  1:161795/1‑71 (MQ=255)                 ttCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGgt                                                       >  1:1575891/1‑71 (MQ=255)                 ttCTGGTCACGACCGTGCATCGGGTTAGCACCCGGGCCGAACGGCGCGCCAGCacgac                                                                    <  1:3335825/58‑1 (MQ=255)                  tgcggTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCgt                                                       <  1:3566356/67‑1 (MQ=255)                  tCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGTCGCGCCAGCACGACGACCGTCTgggg                                                        <  1:3913036/69‑1 (MQ=255)                  tCTGGTCACGACCGCGCATCGGCTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTgggg                                                        <  1:3622849/69‑1 (MQ=255)                      gTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTa                                                   <  1:319054/70‑1 (MQ=255)                      gTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTa                                                   <  1:3237536/70‑1 (MQ=255)                      gTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTAc                                                  >  1:1344474/1‑71 (MQ=255)                      gTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTAc                                                  <  1:802030/71‑1 (MQ=255)                       tCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTa                                                   >  1:3712209/1‑69 (MQ=255)                         aCGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTAccgg                                               >  1:2130713/1‑69 (MQ=255)                           gACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGtt                                             >  1:2110747/1‑71 (MQ=255)                               atGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCt                                         <  1:2165478/70‑1 (MQ=255)                                tGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCtt                                        <  1:2506416/71‑1 (MQ=255)                                  cATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCtggc                                      <  1:536931/71‑4 (MQ=255)                                  cATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTAc                                      >  1:186636/1‑71 (MQ=255)                                   aTCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTa                                       >  1:1333899/1‑69 (MQ=255)                                    tCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGgt                                                       >  1:1597242/1‑52 (MQ=255)                                    tCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa                                    <  1:1570096/71‑1 (MQ=255)                                    tCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa                                    <  1:2027753/71‑1 (MQ=255)                                     cGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa                                    >  1:3414294/1‑70 (MQ=255)                                     cGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa                                    >  1:477167/1‑70 (MQ=255)                                         ttAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTacac                               >  1:1069453/1‑71 (MQ=255)                                         ttAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTAccc                                                <  1:2251354/54‑1 (MQ=255)                                          tAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTggg                                                         >  1:2723754/1‑44 (MQ=255)                                          tAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATacac                               >  1:3024232/1‑70 (MQ=255)                                            gCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAAc                            <  1:3354176/71‑1 (MQ=255)                                            gCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAAc                            >  1:3832078/1‑71 (MQ=255)                                               cccGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGtt                         <  1:1391101/71‑1 (MQ=255)                                               cccGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGt                          >  1:2929608/1‑70 (MQ=255)                                                ccGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTa                                       <  1:2315160/56‑1 (MQ=255)                                                ccGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGt                          >  1:3540482/1‑69 (MQ=255)                                                   gTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAAc                            <  1:435448/64‑1 (MQ=255)                                                   gTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAg                       >  1:3297967/1‑69 (MQ=255)                                                    tCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACAATACACaa                             >  1:2691225/1‑62 (MQ=255)                                                      cGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACAGGTTTTCTGGCCGTACACCACGTTAGAAGt                   <  1:1287419/70‑1 (MQ=255)                                                      cGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGt                   >  1:1936727/1‑70 (MQ=255)                                                       gAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGa                 >  1:3005560/1‑71 (MQ=255)                                                       gAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa                                    <  1:303295/52‑1 (MQ=255)                                                       gAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGa                 <  1:2975766/71‑1 (MQ=255)                                                         aCGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATg               >  1:1373502/1‑71 (MQ=255)                                                         aCGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATg               >  1:2005067/1‑71 (MQ=255)                                                         aCGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATg               >  1:2077016/1‑71 (MQ=255)                                                          cGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATg               >  1:2907238/1‑70 (MQ=255)                                                           ggCGCGCCAGCACGACGACCGTCTGGGGTCTTACCCGTTTTCTTACCATacac                               <  1:273582/53‑1 (MQ=255)                                                             cgcgcCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGt             >  1:2267719/1‑69 (MQ=255)                                                             cgcgcCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGt             >  1:3077494/1‑69 (MQ=255)                                                              gcgcCAGCACGACGACCGTCTGGGGTGTTACCCGttt                                            <  1:2517088/37‑1 (MQ=255)                                                              gcgcCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa                                    >  1:1721901/1‑45 (MQ=255)                                                                gcCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCa                                    >  1:1526192/1‑43 (MQ=255)                                                                gcCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGaa        >  1:30672/1‑71 (MQ=255)                                                                gcCAGCACGACGACCGTCTGGGGCGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGaa        <  1:1787674/71‑1 (MQ=255)                                                                 ccAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAg          <  1:3632670/68‑1 (MQ=255)                                                                   aGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGa         >  1:1684856/1‑67 (MQ=255)                                                                    gCACGTCGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGa    >  1:1463806/1‑71 (MQ=255)                                                                    gCACGACGACCGTCTGGGGTGTTACCCGTTTTCtt                                        >  1:3749690/1‑35 (MQ=255)                                                                    gCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGa    >  1:554475/1‑71 (MQ=255)                                                                    gCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGa    >  1:3029703/1‑71 (MQ=255)                                                                      acgacgACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGaa        >  1:1709023/1‑65 (MQ=255)                                                                      acgacgACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACt  >  1:310005/1‑71 (MQ=255)                                                                      |                                                                       AGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACT  >  minE/608310‑608449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑