Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,667	A→G	6.2%	G529G (GGT→GGC)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,667	0	A	G	6.2%	270.2 / 7.0	97	G529G (GGT→GGC)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base A (43/48);  new base G (3/3);  total (46/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.87e-01

CGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGC  >  minE/608597‑608737                                                                       |                                                                        cGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGa                                                                         <  1:3414100/71‑1 (MQ=255)  gTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGAc                                                                        <  1:1775862/71‑1 (MQ=255)   ttttCGTCACGGATTGGTTTCACGCGGGCATATTTGATGGCAGACAGGGAGTCCGTCGCCACCGACAGGcc                                                                       >  1:1831611/3‑71 (MQ=255)   tCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGAcc                                                                       <  1:1026280/71‑1 (MQ=255)   tCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGAc                                                                        >  1:3168199/1‑70 (MQ=255)     ttCGTCACGGATTGGTTTCACGCGGGCATATTTGATGGCAGACAGGGAGTCCGTCGCCACCGACAGGccc                                                                      <  1:711077/70‑2 (MQ=255)       cGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGa                                                                         >  1:1571016/1‑65 (MQ=255)       cGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCa                                                                      <  1:2657047/68‑1 (MQ=255)       cGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGc                                                                    >  1:557719/1‑70 (MQ=255)       cGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGc                                                                    <  1:3076918/70‑1 (MQ=255)         tCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGa                                                                  >  1:2191647/1‑70 (MQ=255)         tCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGa                                                                  <  1:812721/70‑1 (MQ=255)           aCGAATCGTTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATAc                                                               >  1:2257497/1‑71 (MQ=255)           aCGAATCGTTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATAc                                                               >  1:2262224/1‑71 (MQ=255)             gAATCGGTTTAACTTTCGCATATTTGATTGCAGACTGGGAGTCAGCAGCAACGGACAGAcc                                                                       <  1:3809453/61‑1 (MQ=255)             gAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGAcc                                                                       <  1:2543981/61‑1 (MQ=255)                 cGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCacac                                                          <  1:2421784/70‑1 (MQ=255)                  ggTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGa                                                                  <  1:2445380/61‑1 (MQ=255)                  ggTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGa                                                                  <  1:1038709/61‑1 (MQ=255)                  ggTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACcac                                                            >  1:2081700/1‑67 (MQ=255)                  ggTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCacac                                                          <  1:2426413/69‑1 (MQ=255)                  ggTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGc                                                        >  1:2124819/1‑71 (MQ=255)                   gTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGa                                                                  <  1:3447779/60‑1 (MQ=255)                    tttAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCa                                                      <  1:2145518/71‑1 (MQ=255)                     ttAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCAt                                                     >  1:3191784/1‑71 (MQ=255)                     ttAACTTTCGCATATTTGATTGCAGACA‑GGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                                    >  1:3265956/1‑71 (MQ=255)                      tAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                                    >  1:3395898/1‑71 (MQ=255)                      tAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                                    >  1:1896349/1‑71 (MQ=255)                      tAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                                    >  1:3740986/1‑71 (MQ=255)                        aCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                  <  1:2688667/71‑1 (MQ=255)                        aCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                  >  1:2796511/1‑71 (MQ=255)                        aCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                  <  1:1340474/71‑1 (MQ=255)                        aCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                  <  1:1313648/71‑1 (MQ=255)                        aCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                  <  1:1033046/71‑1 (MQ=255)                         cTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATa                                                                >  1:242166/1‑56 (MQ=255)                         cTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTg                                                 <  1:1379505/71‑1 (MQ=255)                         cTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTg                                                 <  1:3548789/71‑1 (MQ=255)                           ttCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGAc                                                                        >  1:307874/1‑46 (MQ=255)                            tCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                  >  1:335214/1‑67 (MQ=255)                            gggCATATTTGATGGCAGACAGGGAGTCCGTCGCCACCGACAGGCCCGCGATGCCGCATGCCATAGTGCga                                              <  1:97846/69‑2 (MQ=255)                               aatatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTg                                                 <  1:409848/64‑1 (MQ=255)                                atatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                  <  1:1796937/63‑1 (MQ=255)                                atatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                  <  1:1801504/63‑1 (MQ=255)                                atatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCg                                               >  1:3663084/1‑66 (MQ=255)                                atatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCg                                               <  1:1283174/66‑1 (MQ=255)                                 tatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATAcc                                                              <  1:1631657/50‑1 (MQ=255)                                  atTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTg                                                 <  1:3112556/62‑1 (MQ=255)                                  atTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGc                                                >  1:2808678/1‑63 (MQ=255)                                   tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGAGGATa‑a                                         >  1:131469/1‑68 (MQ=255)                                    ttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACg                                       <  1:549217/69‑1 (MQ=255)                                     tGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGa                                                                  >  1:3189177/1‑42 (MQ=255)                                      gATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATa                                                                <  1:809871/43‑1 (MQ=255)                                      gATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATa                                                                <  1:3795398/43‑1 (MQ=255)                                      gATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATa                                                                <  1:3543919/43‑1 (MQ=255)                                      gATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa‑a                                         >  1:3247054/1‑65 (MQ=255)                                      gATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCAc                                   >  1:880896/1‑71 (MQ=255)                                       aTTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACg                                  <  1:1839862/71‑1 (MQ=255)                                          gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGa                                             >  1:517001/1‑58 (MQ=255)                                          gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGt                                <  1:882219/70‑1 (MQ=255)                                          gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTc                               >  1:3093960/1‑71 (MQ=255)                                             gACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                            >  1:989717/1‑71 (MQ=255)                                              aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                      >  1:3476678/1‑60 (MQ=255)                                              aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                            >  1:827740/1‑70 (MQ=255)                                              aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                            >  1:1112320/1‑70 (MQ=255)                                              aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                            >  1:3782036/1‑70 (MQ=255)                                                  ggAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgcg                       >  1:2005090/1‑71 (MQ=255)                                                  ggAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgcg                       <  1:3705642/71‑1 (MQ=255)                                                   gAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCg                                               <  1:3402155/47‑1 (MQ=255)                                                    aGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa                                           <  1:1920507/50‑1 (MQ=255)                                                     gTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCa                    <  1:3249682/71‑1 (MQ=255)                                                     gTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCa                    <  1:2950475/71‑1 (MQ=255)                                                      tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                        >  1:3078752/1‑50 (MQ=255)                                                      tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                        >  1:3074245/1‑50 (MQ=255)                                                      tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCa                    <  1:1277/70‑1 (MQ=255)                                                      tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                   >  1:3428425/1‑71 (MQ=255)                                                      tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                   <  1:609911/71‑1 (MQ=255)                                                        agcagcAATGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                 <  1:98677/71‑1 (MQ=255)                                                        agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                        <  1:2761886/48‑1 (MQ=255)                                                        agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                 <  1:2589152/71‑1 (MQ=255)                                                          cagcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgcg                       <  1:3737942/63‑1 (MQ=255)                                                          cagcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCaga               <  1:1218174/71‑1 (MQ=255)                                                           agcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGcc                     <  1:895464/64‑1 (MQ=255)                                                           agcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagag              <  1:3678985/71‑1 (MQ=255)                                                            gcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                 >  1:220826/1‑67 (MQ=255)                                                              aaCGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                          <  1:2887690/56‑1 (MQ=255)                                                                ccgACAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCt         <  1:1898037/69‑1 (MQ=255)                                                                cGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCt         >  1:3421578/1‑71 (MQ=255)                                                                 ggACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCag                >  1:1146727/1‑63 (MQ=255)                                                                 cgACAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCtt        >  1:150386/2‑71 (MQ=255)                                                                  gACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                            >  1:1831780/1‑50 (MQ=255)                                                                   aCAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCg      >  1:943171/1‑71 (MQ=255)                                                                   aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg      >  1:3799173/1‑71 (MQ=255)                                                                   aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg      >  1:1107471/1‑71 (MQ=255)                                                                   aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg      >  1:3372418/1‑71 (MQ=255)                                                                    cAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg      >  1:3149032/1‑70 (MQ=255)                                                                    cAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGt     <  1:3694058/71‑1 (MQ=255)                                                                     aGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg      >  1:96270/1‑69 (MQ=255)                                                                      gACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCGGAGAGGCTTCGTa    <  1:215811/70‑1 (MQ=255)                                                                      gACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTa    >  1:3193277/1‑70 (MQ=255)                                                                       aCCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGc  <  1:1685785/71‑1 (MQ=255)                                                                       |                                                                        CGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGC  >  minE/608597‑608737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑