Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,688	G→A	10.3%	T522T (ACC→ACT)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,688	0	G	A	10.3%	243.2 / 15.1	87	T522T (ACC→ACT)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base G (37/41);  new base A (4/5);  total (41/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

TTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTA  >  minE/608617‑608756                                                                        |                                                                      ttAACTTTCGCATATTTGATTGCAGACA‑GGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                                                       >  1:3265956/1‑71 (MQ=255)  tAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                                                       >  1:3740986/1‑71 (MQ=255)  tAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                                                       >  1:3395898/1‑71 (MQ=255)  tAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                                                       >  1:1896349/1‑71 (MQ=255)    aCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                                     <  1:1033046/71‑1 (MQ=255)    aCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                                     <  1:1313648/71‑1 (MQ=255)    aCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                                     <  1:2688667/71‑1 (MQ=255)    aCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                                     <  1:1340474/71‑1 (MQ=255)    aCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                                     >  1:2796511/1‑71 (MQ=255)     cTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTg                                                                    <  1:3548789/71‑1 (MQ=255)     cTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTg                                                                    <  1:1379505/71‑1 (MQ=255)        tCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                                     >  1:335214/1‑67 (MQ=255)        gggCATATTTGATGGCAGACAGGGAGTCCGTCGCCACCGACAGGCCCGCGATGCCGCATGCCATAGTGCga                                                                 <  1:97846/69‑2 (MQ=255)           aatatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTg                                                                    <  1:409848/64‑1 (MQ=255)            atatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                                     <  1:1801504/63‑1 (MQ=255)            atatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGt                                                                     <  1:1796937/63‑1 (MQ=255)            atatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCg                                                                  <  1:1283174/66‑1 (MQ=255)            atatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCg                                                                  >  1:3663084/1‑66 (MQ=255)              atTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTg                                                                    <  1:3112556/62‑1 (MQ=255)              atTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGc                                                                   >  1:2808678/1‑63 (MQ=255)               tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGAGGATa‑a                                                            >  1:131469/1‑68 (MQ=255)                ttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACg                                                          <  1:549217/69‑1 (MQ=255)                  gATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa‑a                                                            >  1:3247054/1‑65 (MQ=255)                  gATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCAc                                                      >  1:880896/1‑71 (MQ=255)                   aTTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACg                                                     <  1:1839862/71‑1 (MQ=255)                      gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGa                                                                >  1:517001/1‑58 (MQ=255)                      gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGt                                                   <  1:882219/70‑1 (MQ=255)                      gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTc                                                  >  1:3093960/1‑71 (MQ=255)                         gACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                               >  1:989717/1‑71 (MQ=255)                          aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                                         >  1:3476678/1‑60 (MQ=255)                          aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                               >  1:1112320/1‑70 (MQ=255)                          aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                               >  1:827740/1‑70 (MQ=255)                          aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                               >  1:3782036/1‑70 (MQ=255)                              ggAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgcg                                          >  1:2005090/1‑71 (MQ=255)                              ggAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgcg                                          <  1:3705642/71‑1 (MQ=255)                               gAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCg                                                                  <  1:3402155/47‑1 (MQ=255)                                aGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa                                                              <  1:1920507/50‑1 (MQ=255)                                 gTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCa                                       <  1:2950475/71‑1 (MQ=255)                                 gTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCa                                       <  1:3249682/71‑1 (MQ=255)                                  tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                                           >  1:3074245/1‑50 (MQ=255)                                  tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                                           >  1:3078752/1‑50 (MQ=255)                                  tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCa                                       <  1:1277/70‑1 (MQ=255)                                  tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                      >  1:3428425/1‑71 (MQ=255)                                  tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                      <  1:609911/71‑1 (MQ=255)                                    agcagcAATGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                                    <  1:98677/71‑1 (MQ=255)                                    agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                                           <  1:2761886/48‑1 (MQ=255)                                    agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                                    <  1:2589152/71‑1 (MQ=255)                                      cagcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgcg                                          <  1:3737942/63‑1 (MQ=255)                                      cagcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCaga                                  <  1:1218174/71‑1 (MQ=255)                                       agcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGcc                                        <  1:895464/64‑1 (MQ=255)                                       agcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagag                                 <  1:3678985/71‑1 (MQ=255)                                        gcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                                    >  1:220826/1‑67 (MQ=255)                                          aaCGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                                             <  1:2887690/56‑1 (MQ=255)                                            ccgACAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCt                            <  1:1898037/69‑1 (MQ=255)                                            cGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCt                            >  1:3421578/1‑71 (MQ=255)                                             ggACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCag                                   >  1:1146727/1‑63 (MQ=255)                                             cgACAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCtt                           >  1:150386/2‑71 (MQ=255)                                              gACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                               >  1:1831780/1‑50 (MQ=255)                                               aCAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCg                         >  1:943171/1‑71 (MQ=255)                                               aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                         >  1:1107471/1‑71 (MQ=255)                                               aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                         >  1:3372418/1‑71 (MQ=255)                                               aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                         >  1:3799173/1‑71 (MQ=255)                                                cAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                         >  1:3149032/1‑70 (MQ=255)                                                cAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGt                        <  1:3694058/71‑1 (MQ=255)                                                 aGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                         >  1:96270/1‑69 (MQ=255)                                                  gACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCGGAGAGGCTTCGTa                       <  1:215811/70‑1 (MQ=255)                                                  gACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTa                       >  1:3193277/1‑70 (MQ=255)                                                   aCCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGc                     <  1:1685785/71‑1 (MQ=255)                                                     cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCa                                                       >  1:25645/1‑35 (MQ=255)                                                     cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACgg                                                    <  1:2526365/38‑1 (MQ=255)                                                     cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                                             <  1:2533402/45‑1 (MQ=255)                                                       gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGt                  >  1:2710663/1‑70 (MQ=255)                                                       gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTa                 >  1:194067/1‑71 (MQ=255)                                                       gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTa                 >  1:1630226/1‑71 (MQ=255)                                                        cGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTAc                <  1:2629928/71‑1 (MQ=255)                                                         gATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTAc                <  1:3079912/70‑1 (MQ=255)                                                          aTACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTg                   <  1:1217645/66‑1 (MQ=255)                                                          aTACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACtt              <  1:1694886/71‑1 (MQ=255)                                                           tACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTg             >  1:1711142/1‑71 (MQ=255)                                                            aCCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt            >  1:411857/1‑71 (MQ=255)                                                            aCCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt            >  1:1712925/1‑71 (MQ=255)                                                             ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTc           <  1:3614236/71‑1 (MQ=255)                                                              cacaCGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCg          <  1:256898/71‑1 (MQ=255)                                                                catgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGt         <  1:1649123/67‑1 (MQ=255)                                                                cacGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt            >  1:1554954/1‑67 (MQ=255)                                                                  tgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCa      >  1:2448498/2‑71 (MQ=255)                                                                  tgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCa      >  1:514557/2‑71 (MQ=255)                                                                  cGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTa                 <  1:1576949/60‑1 (MQ=255)                                                                      aTGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATg    >  1:3881228/1‑69 (MQ=255)                                                                      aTGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTa  <  1:448813/71‑1 (MQ=255)                                                                      aTGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTa  <  1:1979514/71‑1 (MQ=255)                                                                        |                                                                      TTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTA  >  minE/608617‑608756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑