BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,772	T→C	32.3%	A494A (GCA→GCG)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,772	0	T	C	32.3%	105.7 / 79.5	93	A494A (GCA→GCG)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base T (36/27); new base C (15/15); total (51/42)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01

Only 100 of 117 total aligned reads displayed.

ACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGT  >  minE/608703‑608839

aCGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtg                                                                    <  1:583284/71‑1 (MQ=255)

aCGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtg                                                                    <  1:3659170/71‑1 (MQ=255)

aCGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtg                                                                    >  1:2448796/1‑71 (MQ=255)

aCGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagt                                                                     >  1:1746528/1‑70 (MQ=255)

 cGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtgc                                                                   <  1:1547791/71‑1 (MQ=255)

  ggTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtg                                                                    >  1:3745091/1‑69 (MQ=255)

   atCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTcagcgcgc                                                                 <  1:2285052/70‑3 (MQ=255)

   atCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTcagcgcgc                                                                 <  1:1986661/70‑3 (MQ=255)

     cGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTcagcgcgct                                                                >  1:3818077/1‑67 (MQ=255)

      gTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtgcagtg                                                               <  1:3364346/70‑1 (MQ=255)

      gTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGa                                                              >  1:524081/1‑71 (MQ=255)

        gCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGAt                                                             <  1:1474046/70‑1 (MQ=255)

         cAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATg                                                            >  1:1270476/1‑70 (MQ=255)

         cAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGt                                                           <  1:1276183/71‑1 (MQ=255)

          aGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTa                                                          >  1:1871441/1‑71 (MQ=255)

            cgcCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACt                                                        >  1:1974860/1‑71 (MQ=255)

              ccATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGt                                                      <  1:2932575/71‑1 (MQ=255)

               cATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGtt                                                     <  1:3283633/71‑1 (MQ=255)

               cATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGtt                                                     <  1:1634670/71‑1 (MQ=255)

               cATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGtt                                                     <  1:535529/71‑1 (MQ=255)

                aTCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTgcac                                                    >  1:1489148/1‑68 (MQ=255)

                 tCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTAc                                                         <  1:1963599/65‑1 (MQ=255)

                 tCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGtt                                                     >  1:233172/1‑69 (MQ=255)

                  cAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTag                                                  >  1:258021/1‑71 (MQ=255)

                   agagagGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagc                                                 >  1:638018/1‑71 (MQ=255)

                    gagagGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagcc                                                <  1:1464048/71‑1 (MQ=255)

                      gagGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccag                                              >  1:3099829/1‑71 (MQ=255)

                       agGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagc                                             >  1:3524790/1‑71 (MQ=255)

                         gCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagc                                             >  1:1012830/1‑69 (MQ=255)

                         gCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagc                                             >  1:3910658/1‑69 (MQ=255)

                           ttCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtgca                                                                  <  1:1460678/46‑1 (MQ=255)

                           ttCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGt                                         >  1:1300864/1‑71 (MQ=255)

                            tCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTa                                                   >  1:2267359/1‑60 (MQ=255)

                             cGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagcca                                               >  1:2269684/1‑63 (MQ=255)

                             cGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGt                                         <  1:972323/69‑1 (MQ=255)

                              gTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCATCGCGCTGATGTACTGCACCGCCAGCCAGTCCa                                      >  1:3113024/1‑71 (MQ=255)

                               tAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCAt                                     <  1:2457256/71‑1 (MQ=255)

                               tAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCAt                                     <  1:3509691/71‑1 (MQ=255)

                                aGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCAt                                     >  1:3492343/1‑70 (MQ=255)

                                 gCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATg                                    <  1:321491/70‑1 (MQ=255)

                                  cTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTg                                                       <  1:563680/50‑1 (MQ=255)

                                  cTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTg                                                       <  1:129318/50‑1 (MQ=255)

                                  cTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATg                                    >  1:1187658/1‑69 (MQ=255)

                                    gTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGt                                >  1:691787/1‑71 (MQ=255)

                                     tACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGt                                                      >  1:3491816/1‑48 (MQ=255)

                                     tACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGt                                >  1:1843002/1‑70 (MQ=255)

                                     tACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGt                                <  1:3668348/70‑1 (MQ=255)

                                     tACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTg                               <  1:2744294/71‑1 (MQ=255)

                                     tACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGt                                >  1:723764/1‑70 (MQ=255)

                                     tACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGt                                >  1:3348768/1‑70 (MQ=255)

                                       cTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGAt                             >  1:1699360/1‑71 (MQ=255)

                                        ttGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATc                            >  1:2524114/1‑71 (MQ=255)

                                         tGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGAt                             >  1:2902988/1‑69 (MQ=255)

                                          gTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATc                            <  1:2918680/69‑1 (MQ=255)

                                           tCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGt                                                           <  1:1612665/37‑1 (MQ=255)

                                           tCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGtt                                                     <  1:2311670/43‑1 (MQ=255)

                                           tCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCa                          <  1:2677928/70‑1 (MQ=255)

                                           tCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCAt                         <  1:2686874/71‑1 (MQ=255)

                                           tCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAg                                 >  1:3728046/1‑63 (MQ=255)

                                           tCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAg                                 >  1:1007084/1‑63 (MQ=255)

                                           tCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATcgag                         >  1:2502151/1‑68 (MQ=255)

                                           tCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATcga                          <  1:726165/70‑3 (MQ=255)

                                            cGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTATCCAGCCAGTCCATGAAGTGATCCATg                        >  1:3262567/1‑71 (MQ=255)

                                                cATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagc                                             <  1:2457636/46‑1 (MQ=255)

                                                cATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTa                                                   >  1:2013503/1‑40 (MQ=255)

                                                cATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCt                    >  1:1615469/1‑71 (MQ=255)

                                                cATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCt                    <  1:2889267/71‑1 (MQ=255)

                                                 aTGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTc                   >  1:787339/1‑71 (MQ=255)

                                                 aTGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTc                   >  1:79017/1‑71 (MQ=255)

                                                 aTGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTc                   <  1:1118954/71‑1 (MQ=255)

                                                 aTGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAg                                 >  1:1027596/1‑57 (MQ=255)

                                                   gTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCa                 >  1:2264940/1‑71 (MQ=255)

                                                    tAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCAt                <  1:2640792/71‑1 (MQ=255)

                                                    tAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCAt                <  1:2956175/71‑1 (MQ=255)

                                                      gTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCAt                >  1:3067653/1‑69 (MQ=255)

                                                       tGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTcc                                       <  1:1230465/45‑1 (MQ=255)

                                                       tGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCAc             <  1:2996861/71‑1 (MQ=255)

                                                       tGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCAc             >  1:2600382/1‑71 (MQ=255)

                                                        ggATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACt            >  1:1342334/1‑71 (MQ=255)

                                                         aatgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCAc             >  1:1476818/2‑69 (MQ=255)

                                                         aatgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtt           >  1:803475/2‑71 (MQ=255)

                                                          atgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATc               <  1:2189933/66‑1 (MQ=255)

                                                           tgatgTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTTATCCATGCGCTCCATCACTtct         >  1:2892586/1‑70 (MQ=255)

                                                           tgatgTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTtca         >  1:2406421/1‑71 (MQ=255)

                                                           tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg         <  1:2592587/71‑3 (MQ=255)

                                                           tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg         <  1:3172816/71‑3 (MQ=255)

                                                           tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg         <  1:179449/71‑3 (MQ=255)

                                                           tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg         >  1:3276593/1‑69 (MQ=255)

                                                           tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg         <  1:1538895/71‑3 (MQ=255)

                                                              tgTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTtcatca      >  1:757589/1‑71 (MQ=255)

                                                               gTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTtcatca      <  1:3148674/70‑1 (MQ=255)

                                                                ttCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCAc             <  1:508204/62‑1 (MQ=255)

                                                                ttCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTcatcat     <  1:674810/70‑1 (MQ=255)

                                                                ttCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTcatcat     <  1:280813/70‑1 (MQ=255)

                                                                 tCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATg                        >  1:3772060/1‑50 (MQ=255)

                                                                  cagtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAg   <  1:521722/70‑1 (MQ=255)

                                                                   agtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGt  <  1:3873760/70‑1 (MQ=255)

                                                                   agtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGt  <  1:2595087/70‑1 (MQ=255)

                                                                   agtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGt  <  1:3743181/70‑1 (MQ=255)

                                                                     tgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTc                   <  1:1168553/51‑1 (MQ=255)

ACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGT  >  minE/608703‑608839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`