BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,776	G→C	32.3%	T493S (ACT→AGT) ‡	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,776	0	G	C	32.3%	109.3 / 78.3	93	T493S (ACT→AGT) ‡	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base G (36/27); new base C (15/15); total (51/42)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01

Only 100 of 110 total aligned reads displayed.

TCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGAC  >  minE/608707‑608846

tCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtgcagt                                                                       >  1:1361555/1‑71 (MQ=255)

  gTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtgcagtg                                                                      <  1:3364346/70‑1 (MQ=255)

  gTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGa                                                                     >  1:3172857/1‑71 (MQ=255)

  gTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGa                                                                     >  1:524081/1‑71 (MQ=255)

    gCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGAt                                                                    <  1:1474046/70‑1 (MQ=255)

     cAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATg                                                                   >  1:1270476/1‑70 (MQ=255)

      aGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTa                                                                 >  1:1871441/1‑71 (MQ=255)

        cgcCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACt                                                               >  1:1974860/1‑71 (MQ=255)

          ccATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGt                                                             <  1:2932575/71‑1 (MQ=255)

           cATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGtt                                                            <  1:3439651/71‑1 (MQ=255)

           cATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGtt                                                            <  1:535529/71‑1 (MQ=255)

           cATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGtt                                                            <  1:1634670/71‑1 (MQ=255)

            aTCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTgcac                                                           >  1:1489148/1‑68 (MQ=255)

             tCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTAc                                                                <  1:1963599/65‑1 (MQ=255)

             tCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGtt                                                            >  1:233172/1‑69 (MQ=255)

              cAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTag                                                         >  1:258021/1‑71 (MQ=255)

               agagagGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagc                                                        >  1:638018/1‑71 (MQ=255)

                gagagGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagcc                                                       <  1:1464048/71‑1 (MQ=255)

                  gagGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccag                                                     >  1:3099829/1‑71 (MQ=255)

                   agGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagc                                                    >  1:3524790/1‑71 (MQ=255)

                     gCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagc                                                    >  1:1012830/1‑69 (MQ=255)

                     gCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagc                                                    >  1:3910658/1‑69 (MQ=255)

                       ttCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGt                                                >  1:1300864/1‑71 (MQ=255)

                        tCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTa                                                          >  1:2267359/1‑60 (MQ=255)

                         cGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagcca                                                      >  1:2269684/1‑63 (MQ=255)

                         cGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGt                                                <  1:972323/69‑1 (MQ=255)

                          gTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCATCGCGCTGATGTACTGCACCGCCAGCCAGTCCa                                             >  1:3113024/1‑71 (MQ=255)

                           tAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCAt                                            <  1:2457256/71‑1 (MQ=255)

                           tAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCAt                                            <  1:3509691/71‑1 (MQ=255)

                            aGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCAt                                            >  1:3492343/1‑70 (MQ=255)

                             gCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATg                                           <  1:321491/70‑1 (MQ=255)

                              cTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTg                                                              <  1:563680/50‑1 (MQ=255)

                              cTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTg                                                              <  1:129318/50‑1 (MQ=255)

                              cTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATg                                           >  1:1187658/1‑69 (MQ=255)

                                gTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGt                                       >  1:691787/1‑71 (MQ=255)

                                 tACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGt                                                             >  1:2960877/1‑48 (MQ=255)

                                 tACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGt                                                             >  1:3491816/1‑48 (MQ=255)

                                 tACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGt                                       >  1:1843002/1‑70 (MQ=255)

                                 tACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGt                                       <  1:3668348/70‑1 (MQ=255)

                                 tACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTg                                      <  1:2744294/71‑1 (MQ=255)

                                 tACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGt                                       >  1:3348768/1‑70 (MQ=255)

                                 tACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGt                                       >  1:723764/1‑70 (MQ=255)

                                   cTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGAt                                    >  1:1699360/1‑71 (MQ=255)

                                   cTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACt                                                               >  1:1219141/1‑44 (MQ=25)

                                    ttGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATc                                   >  1:2524114/1‑71 (MQ=255)

                                     tGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGAt                                    >  1:2902988/1‑69 (MQ=255)

                                      gTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccag                                                     >  1:2458232/1‑51 (MQ=255)

                                      gTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATc                                   <  1:2918680/69‑1 (MQ=255)

                                       tCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGt                                                                  <  1:1544398/37‑1 (MQ=255)

                                       tCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGtt                                                            <  1:2311670/43‑1 (MQ=255)

                                       tCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCa                                 <  1:2677928/70‑1 (MQ=255)

                                       tCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAg                                        >  1:3728046/1‑63 (MQ=255)

                                       tCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAg                                        >  1:1007084/1‑63 (MQ=255)

                                       tCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATcgag                                >  1:2502151/1‑68 (MQ=255)

                                       tCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATcga                                 <  1:726165/70‑3 (MQ=255)

                                        cGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTATCCAGCCAGTCCATGAAGTGATCCATg                               >  1:3262567/1‑71 (MQ=255)

                                            cATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTa                                                          >  1:2013503/1‑40 (MQ=255)

                                            cATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCt                           <  1:2889267/71‑1 (MQ=255)

                                            cATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCt                           >  1:1615469/1‑71 (MQ=255)

                                             aTGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTc                          <  1:1118954/71‑1 (MQ=255)

                                             aTGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTc                          >  1:79017/1‑71 (MQ=255)

                                             aTGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTc                          >  1:787339/1‑71 (MQ=255)

                                             aTGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAg                                        >  1:1027596/1‑57 (MQ=255)

                                               gTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCa                        >  1:2264940/1‑71 (MQ=255)

                                               gTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGAGATCCATGCGCTcc                         >  1:2305669/1‑70 (MQ=255)

                                                tAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCAt                       <  1:2956175/71‑1 (MQ=255)

                                                tAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCAt                       <  1:2640792/71‑1 (MQ=255)

                                                  gTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCAt                       >  1:3067653/1‑69 (MQ=255)

                                                   tGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTcc                                              <  1:1230465/45‑1 (MQ=255)

                                                   tGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCAc                    >  1:2600382/1‑71 (MQ=255)

                                                    ggATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACt                   >  1:1342334/1‑71 (MQ=255)

                                                     aatgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCAc                    >  1:1476818/2‑69 (MQ=255)

                                                     aatgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtt                  >  1:803475/2‑71 (MQ=255)

                                                      atgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATc                      <  1:2189933/66‑1 (MQ=255)

                                                       tgatgTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTTATCCATGCGCTCCATCACTtct                >  1:2892586/1‑70 (MQ=255)

                                                       tgatgTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTtca                >  1:2406421/1‑71 (MQ=255)

                                                       tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg                >  1:3276593/1‑69 (MQ=255)

                                                       tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg                <  1:2592587/71‑3 (MQ=255)

                                                       tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg                <  1:1538895/71‑3 (MQ=255)

                                                       tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg                <  1:3172816/71‑3 (MQ=255)

                                                       tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg                <  1:179449/71‑3 (MQ=255)

                                                          tgTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTtcatca             >  1:757589/1‑71 (MQ=255)

                                                           gTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTtcatca             <  1:3148674/70‑1 (MQ=255)

                                                            ttCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCAc                    <  1:508204/62‑1 (MQ=255)

                                                            ttCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTcatcat            <  1:280813/70‑1 (MQ=255)

                                                            ttCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATACATGCGCTCCATCACTTcatcat            <  1:572039/70‑1 (MQ=255)

                                                              cagtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTtc                 >  1:2811083/1‑63 (MQ=255)

                                                              cagtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAg          <  1:521722/70‑1 (MQ=255)

                                                              cagtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGAGCTCCATCACTTCATCATAGt         >  1:1753131/1‑71 (MQ=255)

                                                               agtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGt         <  1:3873760/70‑1 (MQ=255)

                                                               agtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGt         <  1:3743181/70‑1 (MQ=255)

                                                               agtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGt         <  1:2595087/70‑1 (MQ=255)

                                                                 tgcagtgATGTACTGTTTAGCCAGCCAGTCCATGaa                                         <  1:752392/36‑1 (MQ=255)

                                                                 tgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTc                          <  1:1168553/51‑1 (MQ=255)

                                                                   cagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAgg    <  1:3233660/71‑1 (MQ=255)

                                                                   cagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAgg    <  1:964238/71‑1 (MQ=255)

                                                                   cagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAg     >  1:2603018/1‑70 (MQ=255)

                                                                   cagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAg     <  1:1024179/70‑1 (MQ=255)

                                                                   cagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAg     >  1:1938460/1‑70 (MQ=255)

                                                                     gtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGAc  <  1:494612/71‑1 (MQ=255)

TCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGAC  >  minE/608707‑608846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`