BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,787	T→C	21.9%	K489K (AAA→AAG) ‡	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,787	0	T	C	21.9%	202.4 / 54.7	105	K489K (AAA→AAG) ‡	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base T (42/40); new base C (11/12); total (53/52)
Fisher's exact test for biased strand distribution p-value = 8.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

CCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTT  >  minE/608717‑608855

ccATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGt                                                                      <  1:2932575/71‑1 (MQ=255)

 cATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGtt                                                                     <  1:535529/71‑1 (MQ=255)

 cATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGtt                                                                     <  1:3439651/71‑1 (MQ=255)

 cATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGtt                                                                     <  1:3283633/71‑1 (MQ=255)

 cATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGtt                                                                     <  1:1634670/71‑1 (MQ=255)

    cAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTag                                                                  >  1:258021/1‑71 (MQ=255)

     agagagGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagc                                                                 >  1:638018/1‑71 (MQ=255)

      gagagGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagcc                                                                <  1:1464048/71‑1 (MQ=255)

        gagGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccag                                                              >  1:3099829/1‑71 (MQ=255)

         agGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagc                                                             >  1:3524790/1‑71 (MQ=255)

           gCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagc                                                             >  1:1012830/1‑69 (MQ=255)

           gCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagc                                                             >  1:3910658/1‑69 (MQ=255)

              tCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTa                                                                   >  1:2267359/1‑60 (MQ=255)

               cGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagcca                                                               >  1:2269684/1‑63 (MQ=255)

               cGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGt                                                         <  1:972323/69‑1 (MQ=255)

                gTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCATCGCGCTGATGTACTGCACCGCCAGCCAGTCCa                                                      >  1:3113024/1‑71 (MQ=255)

                 tAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCAt                                                     <  1:3509691/71‑1 (MQ=255)

                 tAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCAt                                                     <  1:2457256/71‑1 (MQ=255)

                  aGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCAt                                                     >  1:3492343/1‑70 (MQ=255)

                   gCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATg                                                    <  1:321491/70‑1 (MQ=255)

                    cTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATg                                                    >  1:1187658/1‑69 (MQ=255)

                      gTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGt                                                >  1:691787/1‑71 (MQ=255)

                       tACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGt                                                                      >  1:2960877/1‑48 (MQ=255)

                       tACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGt                                                >  1:1843002/1‑70 (MQ=255)

                       tACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGt                                                <  1:3668348/70‑1 (MQ=255)

                       tACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTg                                               <  1:2744294/71‑1 (MQ=255)

                       tACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGt                                                >  1:723764/1‑70 (MQ=255)

                       tACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGt                                                >  1:3348768/1‑70 (MQ=255)

                         cTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGAt                                             >  1:1699360/1‑71 (MQ=255)

                           tGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGAt                                             >  1:2902988/1‑69 (MQ=255)

                            gTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccag                                                              >  1:2458232/1‑51 (MQ=255)

                            gTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATc                                            <  1:2918680/69‑1 (MQ=255)

                             tCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGtt                                                                     <  1:2311670/43‑1 (MQ=255)

                             tCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCa                                          <  1:2677928/70‑1 (MQ=255)

                             tCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCAt                                         <  1:2686874/71‑1 (MQ=255)

                             tCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAg                                                 >  1:1007084/1‑63 (MQ=255)

                             tCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAg                                                 >  1:3728046/1‑63 (MQ=255)

                             tCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATcgag                                         >  1:2502151/1‑68 (MQ=255)

                             tCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATcga                                          <  1:726165/70‑3 (MQ=255)

                              cGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTATCCAGCCAGTCCATGAAGTGATCCATg                                        >  1:3262567/1‑71 (MQ=255)

                                  cATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagc                                                             <  1:2457636/46‑1 (MQ=255)

                                  cATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTa                                                                   >  1:2013503/1‑40 (MQ=255)

                                  cATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCt                                    >  1:1615469/1‑71 (MQ=255)

                                   aTGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTc                                   <  1:1118954/71‑1 (MQ=255)

                                   aTGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTc                                   >  1:787339/1‑71 (MQ=255)

                                   aTGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTc                                   >  1:79017/1‑71 (MQ=255)

                                   aTGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAg                                                 >  1:1027596/1‑57 (MQ=255)

                                     gTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCa                                 >  1:2264940/1‑71 (MQ=255)

                                     gTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGAGATCCATGCGCTcc                                  >  1:2305669/1‑70 (MQ=255)

                                      tAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCAt                                <  1:2956175/71‑1 (MQ=255)

                                         tGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTcc                                                       <  1:1230465/45‑1 (MQ=255)

                                         tGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCAc                             <  1:2996861/71‑1 (MQ=255)

                                         tGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCAc                             >  1:2600382/1‑71 (MQ=255)

                                          ggATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACt                            >  1:1342334/1‑71 (MQ=255)

                                           aatgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCAc                             >  1:1476818/2‑69 (MQ=255)

                                           aatgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtt                           >  1:803475/2‑71 (MQ=255)

                                            atgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATc                               <  1:2189933/66‑1 (MQ=255)

                                             tgatgTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTTATCCATGCGCTCCATCACTtct                         >  1:2892586/1‑70 (MQ=255)

                                             tgatgTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTtca                         >  1:2406421/1‑71 (MQ=255)

                                             tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg                         <  1:3172816/71‑3 (MQ=255)

                                             tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg                         <  1:179449/71‑3 (MQ=255)

                                             tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg                         >  1:3276593/1‑69 (MQ=255)

                                             tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg                         <  1:1538895/71‑3 (MQ=255)

                                             tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg                         <  1:2592587/71‑3 (MQ=255)

                                                 gTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTtcatca                      <  1:3148674/70‑1 (MQ=255)

                                                  ttCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCAc                             <  1:508204/62‑1 (MQ=255)

                                                  ttCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTcatcat                     <  1:674810/70‑1 (MQ=255)

                                                  ttCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTcatcat                     <  1:280813/70‑1 (MQ=255)

                                                  ttCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATACATGCGCTCCATCACTTcatcat                     <  1:572039/70‑1 (MQ=255)

                                                    cagtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTtc                          >  1:2811083/1‑63 (MQ=255)

                                                    cagtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAg                   <  1:521722/70‑1 (MQ=255)

                                                    cagtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGAGCTCCATCACTTCATCATAGt                  >  1:1753131/1‑71 (MQ=255)

                                                     agtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGt                  <  1:2595087/70‑1 (MQ=255)

                                                     agtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGt                  <  1:3743181/70‑1 (MQ=255)

                                                     agtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGt                  <  1:3873760/70‑1 (MQ=255)

                                                       tgcagtgATGTACTGTTTAGCCAGCCAGTCCATGaa                                                  <  1:752392/36‑1 (MQ=255)

                                                       tgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTc                                   <  1:1168553/51‑1 (MQ=255)

                                                         cagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAgg             <  1:3233660/71‑1 (MQ=255)

                                                         cagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAgg             <  1:964238/71‑1 (MQ=255)

                                                         cagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAg              <  1:1024179/70‑1 (MQ=255)

                                                         cagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAg              >  1:2603018/1‑70 (MQ=255)

                                                         cagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAg              >  1:1938460/1‑70 (MQ=255)

                                                           gtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGAc           <  1:494612/71‑1 (MQ=255)

                                                               tGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTtcatca                      <  1:2715161/56‑1 (MQ=255)

                                                               tGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATc        >  1:3524696/1‑70 (MQ=255)

                                                                gTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCa                                          >  1:3436955/1‑35 (MQ=255)

                                                                gTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACtt                           <  1:2552218/50‑1 (MQ=255)

                                                                gTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGc      >  1:2636587/1‑71 (MQ=255)

                                                                 tACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGc      <  1:773468/70‑1 (MQ=255)

                                                                 tACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGc      <  1:2925037/70‑1 (MQ=255)

                                                                  aCTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCt    >  1:2696081/1‑71 (MQ=255)

                                                                   cTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCtt   >  1:923880/1‑71 (MQ=255)

                                                                   cTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCt    <  1:2239621/70‑1 (MQ=255)

                                                                   cTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCt    <  1:1595246/70‑1 (MQ=255)

                                                                   cTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCt    <  1:2316637/70‑1 (MQ=255)

                                                                   cTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCt    <  1:447392/70‑1 (MQ=255)

                                                                   cTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCt    <  1:2466096/70‑1 (MQ=255)

                                                                   cTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCt    <  1:3328611/70‑1 (MQ=255)

                                                                    tGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCAt                                <  1:3108930/41‑1 (MQ=255)

                                                                    tGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCttt  >  1:2347368/1‑71 (MQ=255)

CCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTT  >  minE/608717‑608855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`