BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,789	T→C	21.9%	K489E (AAA→GAA) ‡	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,789	0	T	C	21.9%	203.4 / 54.7	105	K489E (AAA→GAA) ‡	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base T (42/40); new base C (11/12); total (53/52)
Fisher's exact test for biased strand distribution p-value = 8.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 106 total aligned reads displayed.

CAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGAT  >  minE/608721‑608858

cAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTag                                                                     >  1:258021/1‑71 (MQ=255)

 agagagGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagc                                                                    >  1:638018/1‑71 (MQ=255)

  gagagGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagcc                                                                   <  1:1464048/71‑1 (MQ=255)

    gagGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccag                                                                 >  1:3099829/1‑71 (MQ=255)

     agGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagc                                                                >  1:3524790/1‑71 (MQ=255)

       gCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagc                                                                >  1:1012830/1‑69 (MQ=255)

       gCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagc                                                                >  1:3910658/1‑69 (MQ=255)

         ttCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGt                                                            >  1:1300864/1‑71 (MQ=255)

          tCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTa                                                                      >  1:2267359/1‑60 (MQ=255)

           cGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagcca                                                                  >  1:2269684/1‑63 (MQ=255)

           cGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGt                                                            <  1:972323/69‑1 (MQ=255)

            gTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCATCGCGCTGATGTACTGCACCGCCAGCCAGTCCa                                                         >  1:3113024/1‑71 (MQ=255)

             tAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCAt                                                        <  1:2457256/71‑1 (MQ=255)

             tAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCAt                                                        <  1:3509691/71‑1 (MQ=255)

              aGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCAt                                                        >  1:3492343/1‑70 (MQ=255)

               gCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATg                                                       <  1:321491/70‑1 (MQ=255)

                cTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATg                                                       >  1:1187658/1‑69 (MQ=255)

                  gTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGt                                                   >  1:691787/1‑71 (MQ=255)

                   tACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGt                                                   >  1:1843002/1‑70 (MQ=255)

                   tACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGt                                                   <  1:3668348/70‑1 (MQ=255)

                   tACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTg                                                  <  1:2744294/71‑1 (MQ=255)

                   tACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGt                                                   >  1:723764/1‑70 (MQ=255)

                   tACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGt                                                   >  1:3348768/1‑70 (MQ=255)

                      ttGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATc                                               >  1:2524114/1‑71 (MQ=255)

                       tGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGAt                                                >  1:2902988/1‑69 (MQ=255)

                        gTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATc                                               <  1:2918680/69‑1 (MQ=255)

                         tCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCa                                             <  1:2677928/70‑1 (MQ=255)

                         tCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCAt                                            <  1:2686874/71‑1 (MQ=255)

                         tCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAg                                                    >  1:3728046/1‑63 (MQ=255)

                         tCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAg                                                    >  1:1007084/1‑63 (MQ=255)

                         tCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATcgag                                            >  1:2502151/1‑68 (MQ=255)

                         tCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATcga                                             <  1:726165/70‑3 (MQ=255)

                              cATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagc                                                                <  1:2457636/46‑1 (MQ=255)

                              cATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTa                                                                      >  1:2013503/1‑40 (MQ=255)

                              cATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCt                                       >  1:1615469/1‑71 (MQ=255)

                              cATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCt                                       <  1:2889267/71‑1 (MQ=255)

                               aTGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTc                                      >  1:787339/1‑71 (MQ=255)

                               aTGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTc                                      >  1:79017/1‑71 (MQ=255)

                               aTGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTc                                      <  1:1118954/71‑1 (MQ=255)

                               aTGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAg                                                    >  1:1027596/1‑57 (MQ=255)

                                 gTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCa                                    >  1:2264940/1‑71 (MQ=255)

                                 gTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGAGATCCATGCGCTcc                                     >  1:2305669/1‑70 (MQ=255)

                                  tAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCAt                                   <  1:2956175/71‑1 (MQ=255)

                                  tAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCAt                                   <  1:2640792/71‑1 (MQ=255)

                                    gTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCAt                                   >  1:3067653/1‑69 (MQ=255)

                                     tGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTcc                                                          <  1:1230465/45‑1 (MQ=255)

                                     tGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCAc                                <  1:2996861/71‑1 (MQ=255)

                                     tGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCAc                                >  1:2600382/1‑71 (MQ=255)

                                      ggATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACt                               >  1:1342334/1‑71 (MQ=255)

                                       aatgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCAc                                >  1:1476818/2‑69 (MQ=255)

                                       aatgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtt                              >  1:803475/2‑71 (MQ=255)

                                        atgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATc                                  <  1:2189933/66‑1 (MQ=255)

                                         tgatgTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTTATCCATGCGCTCCATCACTtct                            >  1:2892586/1‑70 (MQ=255)

                                         tgatgTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTtca                            >  1:2406421/1‑71 (MQ=255)

                                         tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg                            <  1:179449/71‑3 (MQ=255)

                                         tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg                            >  1:3276593/1‑69 (MQ=255)

                                         tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg                            <  1:1538895/71‑3 (MQ=255)

                                         tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg                            <  1:2592587/71‑3 (MQ=255)

                                         tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg                            <  1:3172816/71‑3 (MQ=255)

                                            tgTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTtcatca                         >  1:757589/1‑71 (MQ=255)

                                             gTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTtcatca                         <  1:3148674/70‑1 (MQ=255)

                                              ttCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCAc                                <  1:508204/62‑1 (MQ=255)

                                              ttCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTcatcat                        <  1:674810/70‑1 (MQ=255)

                                              ttCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATACATGCGCTCCATCACTTcatcat                        <  1:572039/70‑1 (MQ=255)

                                               tCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATg                                           >  1:3772060/1‑50 (MQ=255)

                                                cagtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTtc                             >  1:2811083/1‑63 (MQ=255)

                                                cagtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGAGCTCCATCACTTCATCATAGt                     >  1:1753131/1‑71 (MQ=255)

                                                 agtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGt                     <  1:3743181/70‑1 (MQ=255)

                                                 agtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGt                     <  1:2595087/70‑1 (MQ=255)

                                                 agtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGt                     <  1:3873760/70‑1 (MQ=255)

                                                   tgcagtgATGTACTGTTTAGCCAGCCAGTCCATGaa                                                     <  1:752392/36‑1 (MQ=255)

                                                   tgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTc                                      <  1:1168553/51‑1 (MQ=255)

                                                     cagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAgg                <  1:3233660/71‑1 (MQ=255)

                                                     cagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAgg                <  1:964238/71‑1 (MQ=255)

                                                     cagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAg                 >  1:1938460/1‑70 (MQ=255)

                                                     cagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAg                 <  1:1024179/70‑1 (MQ=255)

                                                     cagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAg                 >  1:2603018/1‑70 (MQ=255)

                                                       gtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGAc              <  1:494612/71‑1 (MQ=255)

                                                          aTGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATc           >  1:3248191/1‑71 (MQ=255)

                                                           tGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTtcatca                         <  1:2715161/56‑1 (MQ=255)

                                                           tGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATc           >  1:3524696/1‑70 (MQ=255)

                                                            gTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCa                                             >  1:3436955/1‑35 (MQ=255)

                                                            gTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACtt                              <  1:2552218/50‑1 (MQ=255)

                                                            gTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGc         >  1:2636587/1‑71 (MQ=255)

                                                             tACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGc         <  1:773468/70‑1 (MQ=255)

                                                             tACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGc         <  1:2925037/70‑1 (MQ=255)

                                                              aCTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCt       >  1:536133/1‑71 (MQ=255)

                                                              aCTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCt       >  1:2696081/1‑71 (MQ=255)

                                                               cTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCtt      >  1:923880/1‑71 (MQ=255)

                                                               cTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCt       <  1:1595246/70‑1 (MQ=255)

                                                               cTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCt       <  1:2239444/70‑1 (MQ=255)

                                                               cTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCt       <  1:169843/70‑1 (MQ=255)

                                                               cTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCt       <  1:447392/70‑1 (MQ=255)

                                                               cTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCt       <  1:2239621/70‑1 (MQ=255)

                                                               cTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCt       <  1:2316637/70‑1 (MQ=255)

                                                               cTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCt       <  1:3328611/70‑1 (MQ=255)

                                                               cTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCt       <  1:2466096/70‑1 (MQ=255)

                                                                tGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCAt                                   <  1:3108930/41‑1 (MQ=255)

                                                                tGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCttt     >  1:2347368/1‑71 (MQ=255)

                                                                    tAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGAt  <  1:1463205/70‑1 (MQ=255)

CAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGAT  >  minE/608721‑608858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`