Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 189,466 G→A 10.6% T321T (ACG→ACA ldcC → lysine decarboxylase 2, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE189,4660GA10.6% 234.2 / 14.6 85T321T (ACG→ACAldcClysine decarboxylase 2, constitutive
Reads supporting (aligned to +/- strand):  ref base G (41/34);  new base A (5/4);  total (46/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.71e-01

GTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTAC  >  minE/189398‑189532
                                                                    |                                                                  
gTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGc                                                                   <  1:1536412/70‑1 (MQ=255)
 ttCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTg                                                                 <  1:743585/71‑1 (MQ=255)
  tCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTg                                                                 >  1:2940996/1‑70 (MQ=255)
   cATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGa                                                               >  1:3651934/1‑71 (MQ=255)
        ggTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTcc                                                          >  1:426246/1‑71 (MQ=255)
         gTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTccc                                                         >  1:1472104/1‑71 (MQ=255)
         gTGATCACCAACTCCACATATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTccc                                                         >  1:1073877/1‑71 (MQ=255)
          tGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCg                                                        <  1:1479137/71‑1 (MQ=255)
          tGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCg                                                        >  1:2854688/1‑71 (MQ=255)
            aTCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGt                                                       <  1:18964/70‑1 (MQ=255)
             tCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTc                                                      >  1:1779957/1‑70 (MQ=255)
             tCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCg                                                     <  1:2492462/71‑1 (MQ=255)
             tCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCg                                                     <  1:2126380/71‑1 (MQ=255)
              cACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGa                                                    >  1:518877/1‑71 (MQ=255)
               aCCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGAt                                                   >  1:1186609/1‑71 (MQ=255)
               aCCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGAt                                                   <  1:2671379/71‑1 (MQ=255)
                ccAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGa                                                    <  1:1395885/69‑1 (MQ=255)
                ccAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGAtt                                                  <  1:3814791/71‑1 (MQ=255)
                 cAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGAtt                                                  >  1:108453/1‑70 (MQ=255)
                 cAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTc                                                 <  1:3592842/71‑1 (MQ=255)
                 cAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTc                                                 <  1:1216009/71‑1 (MQ=255)
                  aaCTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGt                                                            >  1:2198093/1‑59 (MQ=255)
                  aaCTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTc                                                      >  1:568710/1‑65 (MQ=255)
                  aaCTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCa                                                >  1:2619085/1‑71 (MQ=255)
                  aaCTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCa                                                >  1:110506/1‑71 (MQ=255)
                   aCTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCAc                                               <  1:3885216/71‑1 (MQ=255)
                      ccACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACtt                                             >  1:1136506/1‑70 (MQ=255)
                       cACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTccc                                                         <  1:1761842/57‑1 (MQ=255)
                       cACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTc                                            <  1:1565380/70‑1 (MQ=255)
                       cACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTc                                            >  1:1987341/1‑70 (MQ=255)
                       cACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTc                                            <  1:124671/70‑1 (MQ=255)
                         ccTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGt                                                       >  1:2022580/1‑57 (MQ=255)
                          cTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGAtt                                        >  1:3353835/1‑71 (MQ=255)
                          cTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGAtt                                        >  1:789431/1‑71 (MQ=255)
                          cTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGAt                                         >  1:647505/1‑70 (MQ=255)
                            atgatgGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGa                                          >  1:2435795/1‑67 (MQ=255)
                               atgGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTcc                                                          >  1:1345790/1‑48 (MQ=255)
                                tgGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCt                                  <  1:2713246/71‑1 (MQ=255)
                                  gCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTgg                                <  1:168469/71‑1 (MQ=255)
                                  gCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTg                                 <  1:2679645/70‑1 (MQ=255)
                                   cTTGCTCTACAACACCGACTGGATCAAACAGACGCTgg                                                                <  1:2525929/38‑1 (MQ=255)
                                   cTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTggg                               <  1:3873571/71‑1 (MQ=255)
                                   cTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTcgg                               <  1:2089225/71‑1 (MQ=255)
                                     tGCTGTACAACACCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTg                             >  1:3499844/1‑71 (MQ=255)
                                      gCTGTACAACACCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTGc                            >  1:87838/1‑71 (MQ=255)
                                        tctACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGa                                          >  1:1899268/1‑55 (MQ=255)
                                          tACAACACCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTGcctt                         <  1:3242697/70‑3 (MQ=255)
                                          tACAACACCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTGCCTTa                        >  1:3923733/1‑71 (MQ=255)
                                          tACAACACCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTGCCTTa                        <  1:3537515/71‑1 (MQ=255)
                                          tACAACACCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTGCCTTa                        >  1:2404752/1‑71 (MQ=255)
                                          tACAACACCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTGCCTTa                        <  1:3480662/71‑1 (MQ=255)
                                          tACAACACCGACTGGATCAAACAGACTCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCg                          <  1:1280254/69‑1 (MQ=255)
                                          tACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCg                          <  1:1474286/69‑1 (MQ=255)
                                          tACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCg                          <  1:1557950/69‑1 (MQ=255)
                                          tACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTa                        >  1:476107/1‑71 (MQ=255)
                                          tACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTa                        >  1:540775/1‑71 (MQ=255)
                                           acaacaCCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTGcctt                         <  1:673216/69‑3 (MQ=255)
                                           acaacaCCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTa                        >  1:2144761/1‑70 (MQ=255)
                                           acaacaCCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTa                        >  1:1123100/1‑70 (MQ=255)
                                            caacaCCGACTTCATCAACAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTGCCTTaca                      >  1:3742465/1‑71 (MQ=255)
                                               cacCGACTGGATCAAACAGACGCTGGATGTCCCGTCGAtt                                                  <  1:424376/40‑1 (MQ=255)
                                                acagACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCa                  >  1:669736/4‑71 (MQ=255)
                                                acCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCa                  >  1:3811930/1‑71 (MQ=255)
                                                acCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACATAGATTCt                                      >  1:3890559/1‑51 (MQ=255)
                                                 ccGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTa                        <  1:59962/64‑1 (MQ=255)
                                                   gACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCAttt               <  1:757113/71‑1 (MQ=255)
                                                   gACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCAttt               <  1:3002159/71‑1 (MQ=255)
                                                    aCTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCAtttt              >  1:2482060/1‑71 (MQ=255)
                                                    aCTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCAtttt              >  1:3379048/1‑71 (MQ=255)
                                                     cTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCAtttt              <  1:3861866/70‑1 (MQ=255)
                                                      tGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTaca                      <  1:1512994/61‑1 (MQ=255)
                                                       ggATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGt                              >  1:781594/1‑52 (MQ=255)
                                                        gATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACAcc                    >  1:755731/1‑61 (MQ=255)
                                                        gATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCa            <  1:2709764/69‑1 (MQ=255)
                                                        gATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCa            <  1:1265227/69‑1 (MQ=255)
                                                        gATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCAt           <  1:1294762/70‑1 (MQ=255)
                                                           cAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTaca                      >  1:1569762/1‑56 (MQ=255)
                                                           cAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTaca                      >  1:416130/1‑56 (MQ=255)
                                                             aaCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATc     <  1:2252234/71‑1 (MQ=255)
                                                             aaCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATc     >  1:382467/1‑71 (MQ=255)
                                                              aCAGACGCTGGATGTCCCGTCGATTGACTTCGATTCTGCCTGGGTGCCGTacac                     >  1:1430428/1‑54 (MQ=255)
                                                              aCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCt    <  1:2792172/71‑1 (MQ=255)
                                                              aCAGACGCTGGATGTCCCGTCGATTCACTTCGATTATGCCTGGGTGCCGTACACCCATTTTCAta          >  1:1193992/1‑64 (MQ=255)
                                                               cAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCg        >  1:2710033/1‑66 (MQ=255)
                                                                  aCGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTAc  >  1:483846/1‑69 (MQ=255)
                                                                    gCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTgg                                >  1:2573694/1‑37 (MQ=255)
                                                                    gCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCa            <  1:872736/57‑1 (MQ=255)
                                                                    |                                                                  
GTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTAC  >  minE/189398‑189532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: