BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,922	C→G	16.0%	L444L (CTG→CTC)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,922	0	C	G	16.0%	255.5 / 33.4	106	L444L (CTG→CTC)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base C (46/43); new base G (8/9); total (54/52)
Fisher's exact test for biased strand distribution p-value = 7.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

CTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTA  >  minE/608852‑608992

cTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTAc                                                                        <  1:2993798/71‑1 (MQ=255)

   tGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAg                                                                      >  1:401538/1‑70 (MQ=255)

   tGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAg                                                                      >  1:3720852/1‑70 (MQ=255)

    gATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGc                                                                     <  1:3535708/70‑1 (MQ=255)

      tCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATg                                                                  >  1:934303/1‑71 (MQ=255)

       cGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATgg                                                                 >  1:3949552/1‑71 (MQ=255)

        ggTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGt                                                                <  1:798967/71‑1 (MQ=255)

        ggTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGt                                                                >  1:2842195/1‑71 (MQ=255)

          ttCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGttt                                                              <  1:3929044/71‑1 (MQ=255)

            cAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGtttt                                                             <  1:2184315/70‑1 (MQ=255)

            cAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTc                                                            >  1:941882/1‑71 (MQ=255)

            cAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTc                                                            >  1:479043/1‑71 (MQ=255)

            cAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTc                                                            >  1:743499/1‑71 (MQ=255)

              gACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGc                                                          <  1:2220937/71‑1 (MQ=255)

                  tCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAg                                                       <  1:2889250/70‑1 (MQ=255)

                   cGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGt                                                     >  1:2065075/1‑71 (MQ=255)

                   cGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGt                                                     >  1:2579454/1‑71 (MQ=255)

                   cGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGt                                                     >  1:2574866/1‑71 (MQ=255)

                    ggACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGt                                                     <  1:1811441/70‑1 (MQ=255)

                       ccgaCCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGc                                                 <  1:485992/68‑1 (MQ=255)

                         gaCCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGcgc                                               <  1:2869311/70‑3 (MQ=255)

                          aCCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAgg                                                      >  1:1137796/1‑63 (MQ=255)

                          aCCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGt                                                     >  1:2764278/1‑64 (MQ=255)

                          aCCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAcg                                              >  1:1475953/1‑71 (MQ=255)

                          aCCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAg                                                       <  1:2463701/62‑1 (MQ=255)

                          aCCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGcgcg                                              <  1:1521300/71‑1 (MQ=255)

                           ccTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAcgc                                             <  1:2996212/71‑1 (MQ=255)

                           ccTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGt                                                     <  1:3059050/63‑1 (MQ=255)

                               cATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCa                                          <  1:2835929/70‑1 (MQ=255)

                               cATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCAc                                         >  1:1781102/1‑71 (MQ=255)

                                 ttttCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGtttt                                                             <  1:833394/49‑1 (MQ=255)

                                 ttttCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCg                                       <  1:921355/71‑1 (MQ=255)

                                  cttCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGt                                                     <  1:3800219/55‑1 (MQ=255)

                                   ttCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATgg                                                                 <  1:3159143/43‑1 (MQ=255)

                                   ttCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGcc                                                         >  1:3053695/1‑51 (MQ=255)

                                   ttCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGcc                                                         >  1:3646875/1‑51 (MQ=255)

                                   ttCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGcc                                                         >  1:1858069/1‑51 (MQ=255)

                                   ttCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCga                                      <  1:3563169/70‑1 (MQ=255)

                                   ttCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCg                                       >  1:3240407/1‑69 (MQ=255)

                                    tCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGttt                                                              >  1:3214221/1‑45 (MQ=255)

                                     cAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACa                                                                       >  1:659043/1‑35 (MQ=255)

                                     cAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACa                                                                       >  1:2858225/1‑35 (MQ=255)

                                     cAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaag                                    <  1:675343/70‑1 (MQ=255)

                                      aGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGtt                                                    <  1:3144932/53‑1 (MQ=255)

                                      aGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaagaa                                  >  1:922123/1‑71 (MQ=255)

                                      aGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaagaa                                  >  1:3825802/1‑71 (MQ=255)

                                       gTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGttt                                                   <  1:2342304/53‑1 (MQ=255)

                                        tttttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTg                                                  >  1:2451095/1‑53 (MQ=255)

                                        tttttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTg                                                  >  1:3416380/1‑53 (MQ=255)

                                        tttttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAc                                               >  1:570867/1‑56 (MQ=255)

                                         ttttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTg                               >  1:3185564/1‑71 (MQ=255)

                                          tttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCa                                                        >  1:1163429/1‑45 (MQ=255)

                                          tttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTg                               >  1:3422021/1‑70 (MQ=255)

                                           ttCGTCAACGCCGCCGTTGATTGCGTACATCATGGTTTTCGcc                                                         <  1:1492905/43‑1 (MQ=255)

                                           ttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCga                                      >  1:1259392/1‑62 (MQ=255)

                                           ttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCa                             >  1:3152497/1‑71 (MQ=255)

                                           ttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCa                             >  1:3789051/1‑71 (MQ=255)

                                            tCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAt                            >  1:311948/1‑71 (MQ=255)

                                             cGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCAt                            <  1:1852187/70‑1 (MQ=255)

                                              gTCCACGCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAtt                           <  1:1468722/70‑1 (MQ=255)

                                              gTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAtt                           <  1:2778101/70‑1 (MQ=255)

                                               tCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCa                                          <  1:2557423/54‑1 (MQ=255)

                                                cAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAc                                               <  1:1149445/48‑1 (MQ=255)

                                                cAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGt                        >  1:206006/1‑71 (MQ=255)

                                                 aaCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCa                                                        >  1:2346010/1‑38 (MQ=255)

                                                 aaCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGtt                       <  1:2912248/71‑1 (MQ=255)

                                                 aaCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGtt                       >  1:2708089/1‑71 (MQ=255)

                                                 aaCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGtt                       >  1:3542483/1‑71 (MQ=255)

                                                 aaCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGt                        <  1:2048483/70‑1 (MQ=255)

                                                 aaCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGt                        <  1:2327653/70‑1 (MQ=255)

                                                 aaCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGt                        <  1:3233016/70‑1 (MQ=255)

                                                 aaCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGG‑TTGCACGCGCAcc                                        >  1:489912/1‑53 (MQ=255)

                                                  aCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGttt                      <  1:2576364/71‑1 (MQ=255)

                                                   cgccgcCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaaga                                   <  1:2662450/57‑1 (MQ=255)

                                                    cccgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGAGCGTGCACCAAAGAACTGCATATgc                        >  1:682230/2‑66 (MQ=255)

                                                     ccgccgTTGATTGCGTACAGCATGGTTTTCGCCAGGt                                                     <  1:1694967/37‑1 (MQ=255)

                                                      cgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTAcc                   >  1:1643088/1‑70 (MQ=255)

                                                       gccgTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCaa                 >  1:2542308/1‑71 (MQ=255)

                                                        ccgTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaag                                    <  1:3901684/51‑1 (MQ=255)

                                                        ccgTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAAc                <  1:3946145/71‑1 (MQ=255)

                                                          gTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCAt                            <  1:2242866/57‑1 (MQ=255)

                                                          gTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGa              >  1:3502839/1‑71 (MQ=255)

                                                          gTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGa              >  1:2423550/1‑71 (MQ=255)

                                                          gTTGATTGCGTACACCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCAt                            <  1:2564288/57‑1 (MQ=255)

                                                             gATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGat             >  1:1525207/1‑69 (MQ=255)

                                                              aTTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCg                                       <  1:2077600/42‑1 (MQ=255)

                                                              aTTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCg                                       <  1:823223/42‑1 (MQ=255)

                                                              aTTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatcat          <  1:3354044/71‑1 (MQ=255)

                                                              aTTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatca           <  1:3315875/70‑1 (MQ=255)

                                                              aTTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatca           <  1:3074143/70‑1 (MQ=255)

                                                              aTTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatca           <  1:2226859/70‑1 (MQ=255)

                                                                 gCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCg                                       <  1:2344491/39‑1 (MQ=255)

                                                                 gCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCg        <  1:3931339/70‑1 (MQ=255)

                                                                  cGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAtt                           >  1:148422/1‑50 (MQ=255)

                                                                  cGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAtt                           >  1:3137387/1‑50 (MQ=255)

                                                                  cGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCggg      >  1:1808979/1‑71 (MQ=255)

                                                                   gTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAAc                                 <  1:1013124/43‑1 (MQ=255)

                                                                   gTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGc     >  1:1335388/1‑71 (MQ=255)

                                                                    tACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCt    <  1:1903957/71‑1 (MQ=255)

                                                                      cAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTa  <  1:3093794/71‑1 (MQ=255)

CTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTA  >  minE/608852‑608992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`