Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 608,943 T→A 24.0% A437A (GCA→GCT)  pflB ← pyruvate formate lyase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE608,9430TA24.0% 208.3 / 65.0 104A437A (GCA→GCT) pflBpyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base T (40/39);  new base A (13/12);  total (53/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

AACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGT  >  minE/608877‑609013
                                                                  |                                                                      
gaCCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGcgc                                                                    <  1:2869311/70‑3 (MQ=255)
 aCCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAcg                                                                   >  1:1475953/1‑71 (MQ=255)
  ccTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAcgc                                                                  <  1:2996212/71‑1 (MQ=255)
   cTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAcgcg                                                                 <  1:3665939/71‑1 (MQ=255)
      cATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCa                                                               <  1:2835929/70‑1 (MQ=255)
      cATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCAc                                                              >  1:1781102/1‑71 (MQ=255)
        ttttCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCg                                                            <  1:921355/71‑1 (MQ=255)
          ttCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCga                                                           <  1:3563169/70‑1 (MQ=255)
          ttCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCg                                                            >  1:3240407/1‑69 (MQ=255)
            cAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaag                                                         >  1:2215457/1‑70 (MQ=255)
            cAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaag                                                         <  1:675343/70‑1 (MQ=255)
             aGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaagaa                                                       >  1:3825802/1‑71 (MQ=255)
             aGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaagaa                                                       >  1:922123/1‑71 (MQ=255)
              gTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGttt                                                                        <  1:2342304/53‑1 (MQ=255)
               tttttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTg                                                                       >  1:3416380/1‑53 (MQ=255)
               tttttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTg                                                                       >  1:2451095/1‑53 (MQ=255)
               tttttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAc                                                                    >  1:570867/1‑56 (MQ=255)
                ttttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTg                                                    >  1:3185564/1‑71 (MQ=255)
                 tttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTg                                                    >  1:3422021/1‑70 (MQ=255)
                  ttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCga                                                           >  1:1259392/1‑62 (MQ=255)
                  ttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCa                                                  >  1:3789051/1‑71 (MQ=255)
                  ttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCa                                                  >  1:3152497/1‑71 (MQ=255)
                   tCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAt                                                 >  1:311948/1‑71 (MQ=255)
                    cGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCAt                                                 <  1:1852187/70‑1 (MQ=255)
                     gTCCACGCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAtt                                                <  1:1468722/70‑1 (MQ=255)
                     gTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAtt                                                <  1:2778101/70‑1 (MQ=255)
                      tCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCa                                                               <  1:2557423/54‑1 (MQ=255)
                       cAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAc                                                                    <  1:1149445/48‑1 (MQ=255)
                       cAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGt                                             >  1:206006/1‑71 (MQ=255)
                        aaCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGtt                                            <  1:2912248/71‑1 (MQ=255)
                        aaCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGtt                                            >  1:3542483/1‑71 (MQ=255)
                        aaCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGt                                             <  1:2327653/70‑1 (MQ=255)
                        aaCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGt                                             <  1:3233016/70‑1 (MQ=255)
                        aaCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGt                                             <  1:2048483/70‑1 (MQ=255)
                        aaCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGG‑TTGCACGCGCAcc                                                             >  1:489912/1‑53 (MQ=255)
                         aCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGttt                                           <  1:2576364/71‑1 (MQ=255)
                          cgccgcCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaaga                                                        <  1:2662450/57‑1 (MQ=255)
                           cccgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGAGCGTGCACCAAAGAACTGCATATgc                                             >  1:682230/2‑66 (MQ=255)
                             cgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGcgcgt                                                                  >  1:2889951/1‑43 (MQ=16)
                             cgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTAcc                                        >  1:1643088/1‑70 (MQ=255)
                              gccgTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCaa                                      >  1:2542308/1‑71 (MQ=255)
                               ccgTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaag                                                         <  1:3901684/51‑1 (MQ=255)
                               ccgTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAAc                                     <  1:3946145/71‑1 (MQ=255)
                                 gTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCAt                                                 <  1:2242866/57‑1 (MQ=255)
                                 gTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGa                                   >  1:2423550/1‑71 (MQ=255)
                                 gTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGa                                   >  1:3502839/1‑71 (MQ=255)
                                 gTTGATTGCGTACACCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCAt                                                 <  1:2564288/57‑1 (MQ=255)
                                    gATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGat                                  >  1:1525207/1‑69 (MQ=255)
                                     aTTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCg                                                            <  1:823223/42‑1 (MQ=255)
                                     aTTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCg                                                            <  1:2077600/42‑1 (MQ=255)
                                     aTTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatcat                               <  1:3354044/71‑1 (MQ=255)
                                     aTTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatca                                <  1:3315875/70‑1 (MQ=255)
                                     aTTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatca                                <  1:2226859/70‑1 (MQ=255)
                                        gCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCg                                                            <  1:2344491/39‑1 (MQ=255)
                                        gCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCg                             <  1:3931339/70‑1 (MQ=255)
                                         cGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAtt                                                >  1:148422/1‑50 (MQ=255)
                                         cGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAtt                                                >  1:3137387/1‑50 (MQ=255)
                                         cGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCggg                           >  1:1808979/1‑71 (MQ=255)
                                          gTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAAc                                                      <  1:1013124/43‑1 (MQ=255)
                                          gTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGc                          >  1:1335388/1‑71 (MQ=255)
                                           tACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCt                         <  1:1903957/71‑1 (MQ=255)
                                             cAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTa                       <  1:3093794/71‑1 (MQ=255)
                                               gCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGtt                                            <  1:2632525/48‑1 (MQ=255)
                                               gCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCggg                           <  1:1882474/65‑1 (MQ=255)
                                               gCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCgg                            >  1:3197307/1‑64 (MQ=255)
                                                  tGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACgcag                  >  1:2136562/1‑71 (MQ=255)
                                                  gcgTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcag                  <  1:574363/69‑1 (MQ=255)
                                                   cgTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcag                  <  1:1296101/69‑1 (MQ=255)
                                                    gTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTAcgccgc                 <  1:1695153/70‑1 (MQ=255)
                                                    gTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACgcagca                <  1:256287/71‑1 (MQ=255)
                                                    gTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACgcagc                 <  1:2876279/70‑1 (MQ=255)
                                                    gTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACgcagc                 >  1:3293049/1‑70 (MQ=255)
                                                     ttttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACgcagca                <  1:453039/70‑1 (MQ=255)
                                                     ttttCGCCAGGTTAGCGCGTGCACCAAAGAACTGc                                                   <  1:300387/35‑1 (MQ=16)
                                                     ttttCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcagcag               >  1:3433081/1‑70 (MQ=255)
                                                      tttCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcagcagg              >  1:3199830/1‑69 (MQ=255)
                                                       ttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAAGGATCATCGGGCTTACGCagcaag              >  1:1778673/1‑70 (MQ=255)
                                                       ttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCgg                            >  1:3470676/1‑56 (MQ=255)
                                                       ttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaag              >  1:1305649/1‑70 (MQ=255)
                                                       ttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaag              >  1:527550/1‑70 (MQ=255)
                                                       ttCGCCAGGTTTGCACGCGCACCGAACAACTGCAt                                                 >  1:2062714/1‑35 (MQ=255)
                                                       ttCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAttt                                               >  1:1313612/1‑37 (MQ=21)
                                                        tCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCagcaggc             >  1:3820084/1‑70 (MQ=255)
                                                         cGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcag                  <  1:351852/64‑1 (MQ=255)
                                                           ccAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAAtc         <  1:2743812/71‑2 (MQ=255)
                                                            cAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatca                                >  1:2448292/1‑47 (MQ=255)
                                                            cAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatca                                >  1:2690320/1‑47 (MQ=255)
                                                            cAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatca                                >  1:434905/1‑47 (MQ=255)
                                                            cAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatca                                >  1:437652/1‑47 (MQ=255)
                                                             aGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTa                                          >  1:3778134/1‑36 (MQ=255)
                                                             aGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGc       >  1:746943/1‑71 (MQ=255)
                                                             aGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGc       >  1:1523287/1‑71 (MQ=255)
                                                              ggTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCATCAAGCAATAGCg      >  1:3409611/1‑71 (MQ=255)
                                                              ggTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCg      >  1:1617089/1‑71 (MQ=255)
                                                              ggTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATcgcg      >  1:584689/1‑71 (MQ=255)
                                                               gTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAAc                                     <  1:2842939/39‑1 (MQ=255)
                                                                tttGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCg      <  1:3466562/69‑1 (MQ=255)
                                                                 ttGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAg   >  1:3045171/1‑71 (MQ=255)
                                                                  tGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAg   <  1:1345190/70‑1 (MQ=255)
                                                                  tGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGt  >  1:1450037/1‑71 (MQ=255)
                                                                  |                                                                      
AACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGT  >  minE/608877‑609013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑