| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 189,478 | G→A | 10.7% | P325P (CCG→CCA) ‡ | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 189,478 | 0 | G | A | 10.7% | 235.9 / 14.7 | 84 | P325P (CCG→CCA) ‡ | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (38/37); new base A (5/4); total (43/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01 | |||||||||||
TGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTA > minE/189408‑189548 | tGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCg > 1:2854688/1‑71 (MQ=255)tGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCg < 1:1479137/71‑1 (MQ=255) aTCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGt < 1:18964/70‑1 (MQ=255) tCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTc > 1:1779957/1‑70 (MQ=255) tCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCg < 1:2126380/71‑1 (MQ=255) tCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCg < 1:2492462/71‑1 (MQ=255) cACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGa > 1:518877/1‑71 (MQ=255) aCCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGAt > 1:1186609/1‑71 (MQ=255) aCCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGAt < 1:2671379/71‑1 (MQ=255) ccAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGa < 1:1395885/69‑1 (MQ=255) ccAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGAtt < 1:3814791/71‑1 (MQ=255) cAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGAtt > 1:108453/1‑70 (MQ=255) cAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTc < 1:1216009/71‑1 (MQ=255) cAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTc < 1:3592842/71‑1 (MQ=255) aaCTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTc > 1:568710/1‑65 (MQ=255) aaCTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCa > 1:2619085/1‑71 (MQ=255) aaCTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCa > 1:110506/1‑71 (MQ=255) aCTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCAc < 1:3885216/71‑1 (MQ=255) ccACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACtt > 1:1136506/1‑70 (MQ=255) cACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTc < 1:1565380/70‑1 (MQ=255) cACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTc < 1:124671/70‑1 (MQ=255) cACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTc > 1:1987341/1‑70 (MQ=255) ccTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGt > 1:2022580/1‑57 (MQ=255) cTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGAtt > 1:3353835/1‑71 (MQ=255) cTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGAtt > 1:789431/1‑71 (MQ=255) cTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGAt > 1:647505/1‑70 (MQ=255) atgatgGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGa > 1:2435795/1‑67 (MQ=255) tgGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCt < 1:2713246/71‑1 (MQ=255) gCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTgg < 1:168469/71‑1 (MQ=255) gCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTg < 1:2679645/70‑1 (MQ=255) cTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTggg < 1:3873571/71‑1 (MQ=255) cTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTcgg < 1:2089225/71‑1 (MQ=255) tGCTGTACAACACCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTg > 1:3499844/1‑71 (MQ=255) gCTGTACAACACCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTGc > 1:87838/1‑71 (MQ=255) tctACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGa > 1:1899268/1‑55 (MQ=255) tACAACACCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTGcctt < 1:3242697/70‑3 (MQ=255) tACAACACCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTGCCTTa < 1:3537515/71‑1 (MQ=255) tACAACACCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTGCCTTa < 1:3480662/71‑1 (MQ=255) tACAACACCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTGCCTTa > 1:2404752/1‑71 (MQ=255) tACAACACCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTGCCTTa > 1:3923733/1‑71 (MQ=255) tACAACACCGACTGGATCAAACAGACTCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCg < 1:1280254/69‑1 (MQ=255) tACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCg < 1:1474286/69‑1 (MQ=255) tACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCg < 1:1557950/69‑1 (MQ=255) tACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTa > 1:540775/1‑71 (MQ=255) tACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTa > 1:476107/1‑71 (MQ=255) acaacaCCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTGcctt < 1:673216/69‑3 (MQ=255) acaacaCCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTa > 1:2144761/1‑70 (MQ=255) acaacaCCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTa > 1:1123100/1‑70 (MQ=255) caacaCCGACTTCATCAACAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTGCCTTaca > 1:3742465/1‑71 (MQ=255) cacCGACTGGATCAAACAGACGCTGGATGTCCCGTCGAtt < 1:424376/40‑1 (MQ=255) acagACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCa > 1:669736/4‑71 (MQ=255) acCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCa > 1:3811930/1‑71 (MQ=255) acCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACATAGATTCt > 1:3890559/1‑51 (MQ=255) ccGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTa < 1:59962/64‑1 (MQ=255) gACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCAttt < 1:3002159/71‑1 (MQ=255) gACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCAttt < 1:757113/71‑1 (MQ=255) aCTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCAtttt > 1:3379048/1‑71 (MQ=255) aCTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCAtttt > 1:2482060/1‑71 (MQ=255) cTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCAtttt < 1:3861866/70‑1 (MQ=255) tGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTaca < 1:1512994/61‑1 (MQ=255) ggATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGt > 1:781594/1‑52 (MQ=255) gATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACAcc > 1:755731/1‑61 (MQ=255) gATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCa < 1:1265227/69‑1 (MQ=255) gATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCa < 1:2709764/69‑1 (MQ=255) gATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCAt < 1:1294762/70‑1 (MQ=255) cAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTaca > 1:1569762/1‑56 (MQ=255) cAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTaca > 1:416130/1‑56 (MQ=255) aaCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATc > 1:382467/1‑71 (MQ=255) aaCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATc < 1:2252234/71‑1 (MQ=255) aCAGACGCTGGATGTCCCGTCGATTGACTTCGATTCTGCCTGGGTGCCGTacac > 1:1430428/1‑54 (MQ=255) aCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCt < 1:2792172/71‑1 (MQ=255) aCAGACGCTGGATGTCCCGTCGATTCACTTCGATTATGCCTGGGTGCCGTACACCCATTTTCAta > 1:1193992/1‑64 (MQ=255) cAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCg > 1:2710033/1‑66 (MQ=255) aCGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTAc > 1:483846/1‑69 (MQ=255) gCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTgg > 1:2573694/1‑37 (MQ=255) gCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCa < 1:872736/57‑1 (MQ=255) gATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTa > 1:651933/1‑70 (MQ=255) gATGT‑CCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTaaa < 1:667737/71‑1 (MQ=255) aTGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTaaa < 1:3153832/71‑1 (MQ=255) tCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGt < 1:2497198/71‑1 (MQ=255) ccGTCGATTCACTTCGATTCTGCCTGGGTGGCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTgg > 1:3355659/1‑71 (MQ=255) ccGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGAt > 1:683974/1‑53 (MQ=255) ccGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCATGGTaa < 1:2600200/65‑1 (MQ=255) ccGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTgg < 1:3386711/71‑1 (MQ=255) ccGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTgg > 1:205432/1‑71 (MQ=255) ccGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTg < 1:1167861/70‑1 (MQ=255) gTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAggg > 1:2070441/1‑60 (MQ=255) gTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTa > 1:3089411/1‑71 (MQ=255) | TGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTA > minE/189408‑189548 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |