Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	609,739	G→C	19.9%	A172G (GCA→GGA) ‡	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	609,739	0	G	C	19.9%	188.8 / 38.5	90	A172G (GCA→GGA) ‡	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base G (41/31);  new base C (10/8);  total (51/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACA  >  minE/609671‑609808                                                                     |                                                                      tcatcaGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCa                                                                     >  1:2098277/1‑71 (MQ=255)  catcaGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCAt                                                                    >  1:3428461/1‑71 (MQ=255)    tcaGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCt                                                                  <  1:2305776/71‑1 (MQ=255)      aGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTgg                                                                >  1:1033102/1‑71 (MQ=255)      aGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTgg                                                                >  1:3538684/1‑71 (MQ=255)       ggTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTgg                                                                >  1:1976427/1‑70 (MQ=255)        gTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCa                                                              <  1:2268395/71‑1 (MQ=255)          aGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGa                                                            >  1:878512/1‑71 (MQ=255)           gTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATc                                                                   >  1:3429532/1‑63 (MQ=255)           gTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATc                                                                   >  1:2225540/1‑63 (MQ=255)           gTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGa                                                            <  1:1835672/70‑1 (MQ=255)           gTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGAc                                                           >  1:1429495/1‑71 (MQ=255)           gTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGAc                                                           >  1:2999190/1‑71 (MQ=255)            ctgATGCCATACAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTg                                                                 >  1:208337/3‑64 (MQ=255)            ctgATGCCATACAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTg                                                                 >  1:941580/3‑64 (MQ=255)              gATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCgg                                                        >  1:772263/1‑71 (MQ=255)              gATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCgg                                                        >  1:1975028/1‑71 (MQ=255)              gATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCgg                                                        <  1:651234/71‑1 (MQ=255)              gATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCg                                                         <  1:3947610/70‑1 (MQ=255)                tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGAc                                                           >  1:1150230/1‑66 (MQ=255)                 aCCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCgg                                                        >  1:3559219/1‑68 (MQ=255)                 aCCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGt                                                       >  1:2029271/1‑69 (MQ=255)                  ccGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGAATCTGGCAGACCGGTCa                                                     <  1:2138738/70‑1 (MQ=255)                   cGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGa                                                   <  1:614699/71‑1 (MQ=255)                    gTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGaa                                                  <  1:2199452/71‑1 (MQ=255)                       cAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGaa                                                  <  1:1353960/68‑1 (MQ=255)                        aGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGcc                                              <  1:3794751/71‑1 (MQ=255)                           gctaCGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGa                                           <  1:2344681/68‑1 (MQ=255)                           gcAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCt                                                                  <  1:1224188/48‑1 (MQ=255)                              aCGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACAcc                                              <  1:3513508/65‑1 (MQ=255)                              aCGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGAttt                                        <  1:3367157/71‑1 (MQ=255)                               cgcgACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTa                                       <  1:3746778/71‑1 (MQ=255)                               cgcgACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTa                                       >  1:1437964/1‑71 (MQ=255)                                   aCGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACgg                                    >  1:3381941/1‑70 (MQ=255)                                    cGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGc                                   >  1:2347202/1‑70 (MQ=255)                                     ggTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCaa                                 >  1:277157/1‑71 (MQ=255)                                       tAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACg                               <  1:3363619/71‑1 (MQ=255)                                         gTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCg                               >  1:1254477/1‑69 (MQ=255)                                          tCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGAtt                                         <  1:2711657/58‑1 (MQ=255)                                          tCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTAc                                      <  1:3535560/61‑1 (MQ=255)                                          tCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAg                            <  1:2118094/71‑1 (MQ=255)                                            aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCa                                                     >  1:2631320/1‑44 (MQ=255)                                            aCCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAgc                                                   <  1:3941157/46‑2 (MQ=255)                                             ccGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGAt                         <  1:1036060/71‑1 (MQ=255)                                             ccGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGt                                                       <  1:2146723/41‑1 (MQ=255)                                              cGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAg                            >  1:2603756/1‑67 (MQ=255)                                              cGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAg                            >  1:762822/1‑67 (MQ=255)                                              cGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATg                        >  1:1591625/1‑71 (MQ=255)                                              cGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGAtt                                         <  1:1316290/54‑1 (MQ=255)                                               gatgatACGGCCACGGCCATATGGATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGt                       >  1:3618208/1‑71 (MQ=255)                                               gatgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGt                       >  1:2490979/1‑71 (MQ=255)                                               gataatGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCAtat                       <  1:3795941/71‑3 (MQ=255)                                                atgatACGGCCACGGCCATATGCATCTGGCAGACCGGTc                                                      >  1:2591318/1‑39 (MQ=255)                                                atgatACGGCCACGGCCATATGCATCTGGCAGACCGGTc                                                      >  1:1744646/1‑39 (MQ=255)                                                atgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGc                              >  1:1713932/1‑63 (MQ=255)                                                atgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGc                              >  1:563614/1‑63 (MQ=255)                                                atgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTc                      >  1:8738/1‑71 (MQ=255)                                                atgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAAGAtag                       >  1:1777735/1‑68 (MQ=255)                                                   atACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCgg                   <  1:2110042/71‑1 (MQ=255)                                                   atACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCg                    <  1:264934/70‑1 (MQ=255)                                                    tACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCg                    >  1:403082/1‑69 (MQ=255)                                                    tACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCg                    >  1:1819363/1‑69 (MQ=255)                                                       ggccacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAg                 <  1:3154099/69‑1 (MQ=255)                                                        gccacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCa                             <  1:681140/56‑1 (MQ=255)                                                        cccacggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAgc                           >  1:2368655/2‑57 (MQ=255)                                                         ccacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAgtgt              >  1:2180042/1‑70 (MQ=255)                                                         ccacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAgt                >  1:2812195/1‑68 (MQ=255)                                                          cacggccaTATGCATGTGGCAGACCGGTCAGAACACCAGAtt                                         <  1:1626566/42‑1 (MQ=255)                                                          cacggccaTATGCATCTGTCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaa            <  1:5535/71‑1 (MQ=255)                                                          cacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGAtt                                         <  1:3645305/42‑1 (MQ=255)                                                          cacggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaa            <  1:3006292/71‑1 (MQ=255)                                                           acggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa           >  1:1205796/1‑71 (MQ=255)                                                            cggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa             <  1:1759126/68‑1 (MQ=255)                                                            cggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa             >  1:2006983/1‑68 (MQ=255)                                                            cggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa           >  1:2683014/1‑70 (MQ=255)                                                            cggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa           >  1:3015164/1‑70 (MQ=255)                                                            cggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa           >  1:2393813/1‑70 (MQ=255)                                                            cggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa           >  1:2389073/1‑70 (MQ=255)                                                             tgccaGATGCATATGGCAGACAGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACg         >  1:2894211/2‑71 (MQ=255)                                                             ggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACg         >  1:96175/1‑71 (MQ=255)                                                             ggccaTATGCATCTGGCAGACAGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACg         <  1:1154638/71‑1 (MQ=255)                                                               ccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAAcat        >  1:3254109/1‑68 (MQ=255)                                                                  tatGCATCTGGCAGACCGGTCAGAACACCAGATTTACgg                                    <  1:735503/39‑1 (MQ=255)                                                                  tatGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAg                            <  1:1244351/47‑1 (MQ=255)                                                                   atGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGa                  >  1:3667396/1‑56 (MQ=255)                                                                   atGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGa                  >  1:2681569/1‑56 (MQ=255)                                                                    tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaaa           >  1:89121/1‑62 (MQ=255)                                                                    tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca  <  1:3863764/71‑1 (MQ=255)                                                                    tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca  <  1:3713503/71‑1 (MQ=255)                                                                    tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca  <  1:1557305/71‑1 (MQ=255)                                                                     gCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGa                  >  1:2082483/1‑54 (MQ=255)                                                                     |                                                                      TCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACA  >  minE/609671‑609808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑