Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	609,749	G→A	21.2%	L169L (CTG→TTG) ‡	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	609,749	0	G	A	21.2%	206.6 / 47.1	99	L169L (CTG→TTG) ‡	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base G (46/32);  new base A (11/10);  total (57/42)
Fisher's exact test for biased strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCC  >  minE/609680‑609813                                                                      |                                                                 aGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGa                                                                 >  1:878512/1‑71 (MQ=255)  gTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGa                                                                 <  1:1835672/70‑1 (MQ=255)  gTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGAc                                                                >  1:2999190/1‑71 (MQ=255)  gTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGAc                                                                >  1:1429495/1‑71 (MQ=255)     gATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCgg                                                             >  1:772263/1‑71 (MQ=255)     gATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCgg                                                             <  1:651234/71‑1 (MQ=255)     gATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCgg                                                             >  1:1975028/1‑71 (MQ=255)     gATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCg                                                              <  1:3947610/70‑1 (MQ=255)       tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGAc                                                                >  1:1150230/1‑66 (MQ=255)        aCCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCgg                                                             >  1:3559219/1‑68 (MQ=255)        aCCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGt                                                            >  1:2029271/1‑69 (MQ=255)         ccGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGAATCTGGCAGACCGGTCa                                                          <  1:2138738/70‑1 (MQ=255)          cGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGa                                                        <  1:614699/71‑1 (MQ=255)           gTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGaa                                                       <  1:2199452/71‑1 (MQ=255)              cAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGaa                                                       <  1:1353960/68‑1 (MQ=255)               aGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGcc                                                   <  1:3794751/71‑1 (MQ=255)                  gctaCGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGa                                                <  1:2344681/68‑1 (MQ=255)                     aCGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACAcc                                                   <  1:3513508/65‑1 (MQ=255)                     aCGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGAttt                                             <  1:3367157/71‑1 (MQ=255)                      cgcgACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTa                                            >  1:1437964/1‑71 (MQ=255)                      cgcgACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTa                                            <  1:3746778/71‑1 (MQ=255)                          aCGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACgg                                         >  1:3381941/1‑70 (MQ=255)                           cGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGc                                        >  1:2347202/1‑70 (MQ=255)                            ggTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCaa                                      >  1:277157/1‑71 (MQ=255)                              tAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACg                                    <  1:3363619/71‑1 (MQ=255)                                gTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCg                                    >  1:1254477/1‑69 (MQ=255)                                 tCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGAtt                                              <  1:2711657/58‑1 (MQ=255)                                 tCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTAc                                           <  1:3535560/61‑1 (MQ=255)                                 tCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAg                                 <  1:2118094/71‑1 (MQ=255)                                   aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCa                                                          >  1:2631320/1‑44 (MQ=255)                                   aCCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAgc                                                        <  1:3941157/46‑2 (MQ=255)                                    ccGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGAt                              <  1:1036060/71‑1 (MQ=255)                                    ccGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGt                                                            <  1:2146723/41‑1 (MQ=255)                                     cGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAg                                 >  1:762822/1‑67 (MQ=255)                                     cGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAg                                 >  1:2603756/1‑67 (MQ=255)                                     cGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATg                             >  1:1591625/1‑71 (MQ=255)                                     cGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGAtt                                              <  1:1316290/54‑1 (MQ=255)                                      gatgatACGGCCACGGCCATATGGATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGt                            >  1:3618208/1‑71 (MQ=255)                                      gatgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGt                            >  1:2490979/1‑71 (MQ=255)                                      gataatGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCAtat                            <  1:3795941/71‑3 (MQ=255)                                       atgatACGGCCACGGCCATATGCATCTGGCAGACCGGTc                                                           >  1:2591318/1‑39 (MQ=255)                                       atgatACGGCCACGGCCATATGCATCTGGCAGACCGGTc                                                           >  1:1744646/1‑39 (MQ=255)                                       atgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGc                                   >  1:563614/1‑63 (MQ=255)                                       atgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGc                                   >  1:1713932/1‑63 (MQ=255)                                       atgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTc                           >  1:8738/1‑71 (MQ=255)                                       atgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAAGAtag                            >  1:1777735/1‑68 (MQ=255)                                          atACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCgg                        <  1:2110042/71‑1 (MQ=255)                                          atACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCg                         <  1:264934/70‑1 (MQ=255)                                           tACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCg                         >  1:403082/1‑69 (MQ=255)                                           tACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCg                         >  1:1819363/1‑69 (MQ=255)                                              ggccacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAg                      <  1:3154099/69‑1 (MQ=255)                                               gccacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCa                                  <  1:681140/56‑1 (MQ=255)                                               cccacggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAgc                                >  1:2368655/2‑57 (MQ=255)                                                ccacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAgtgt                   >  1:2180042/1‑70 (MQ=255)                                                ccacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAgt                     >  1:2812195/1‑68 (MQ=255)                                                 cacggccaTATGCATGTGGCAGACCGGTCAGAACACCAGAtt                                              <  1:1626566/42‑1 (MQ=255)                                                 cacggccaTATGCATCTGTCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaa                 <  1:5535/71‑1 (MQ=255)                                                 cacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGAtt                                              <  1:3645305/42‑1 (MQ=255)                                                 cacggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaa                 <  1:3006292/71‑1 (MQ=255)                                                  acggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                >  1:1205796/1‑71 (MQ=255)                                                   cggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa                  <  1:1759126/68‑1 (MQ=255)                                                   cggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa                  >  1:2006983/1‑68 (MQ=255)                                                   cggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                >  1:3015164/1‑70 (MQ=255)                                                   cggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                >  1:2683014/1‑70 (MQ=255)                                                   cggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                >  1:2393813/1‑70 (MQ=255)                                                   cggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                >  1:2389073/1‑70 (MQ=255)                                                    tgccaGATGCATATGGCAGACAGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACg              >  1:2894211/2‑71 (MQ=255)                                                    ggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACg              >  1:96175/1‑71 (MQ=255)                                                    ggccaTATGCATCTGGCAGACAGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACg              <  1:1154638/71‑1 (MQ=255)                                                      ccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAAcat             >  1:3254109/1‑68 (MQ=255)                                                         tatGCATCTGGCAGACCGGTCAGAACACCAGATTTACgg                                         <  1:735503/39‑1 (MQ=255)                                                         tatGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAg                                 <  1:1244351/47‑1 (MQ=255)                                                          atGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGa                       >  1:3667396/1‑56 (MQ=255)                                                          atGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGa                       >  1:2681569/1‑56 (MQ=255)                                                           tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaaa                >  1:89121/1‑62 (MQ=255)                                                           tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca       <  1:3713503/71‑1 (MQ=255)                                                           tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca       <  1:1557305/71‑1 (MQ=255)                                                           tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca       <  1:3863764/71‑1 (MQ=255)                                                            gCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGa                       >  1:2082483/1‑54 (MQ=255)                                                            ccATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCggt                       >  1:1971649/2‑53 (MQ=16)                                                             cATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACg     <  1:3294142/71‑1 (MQ=255)                                                             cATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaa                 >  1:2722572/1‑59 (MQ=255)                                                             cATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaa                 >  1:3389602/1‑59 (MQ=255)                                                              aTCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATcaa          <  1:2573065/65‑3 (MQ=255)                                                              aTCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGc    >  1:934271/1‑71 (MQ=255)                                                              aTCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAg                      >  1:84688/1‑53 (MQ=255)                                                              aTCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGa          >  1:3472631/1‑65 (MQ=255)                                                              aTCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGa          >  1:622401/1‑65 (MQ=255)                                                               tCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAg                                 >  1:2598261/1‑41 (MQ=19)                                                               tCTGGCAGACCGGTCAGAACACCAGATTTACGGCa                                       <  1:398088/35‑1 (MQ=255)                                                                cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaaa                >  1:75521/1‑57 (MQ=255)                                                                cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaaa                >  1:430826/1‑57 (MQ=255)                                                                cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc  >  1:683712/1‑71 (MQ=255)                                                                cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc  >  1:1200076/1‑71 (MQ=255)                                                                cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc  >  1:3202735/1‑71 (MQ=255)                                                                cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc  >  1:3124160/1‑71 (MQ=255)                                                                  ggCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGa          <  1:3232434/61‑1 (MQ=255)                                                                   gCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGa          <  1:2694772/60‑1 (MQ=255)                                                                     aGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa                  <  1:1952353/50‑1 (MQ=255)                                                                      |                                                                 AGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCC  >  minE/609680‑609813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑