Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	609,762	A→G	21.9%	G164G (GGT→GGC)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	609,762	0	A	G	21.9%	185.6 / 49.6	97	G164G (GGT→GGC)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base A (39/36);  new base G (13/8);  total (52/45)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

AGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGG  >  minE/609694‑609831                                                                     |                                                                      aGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGcc                                                                     <  1:3794751/71‑1 (MQ=255)    gctaCGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGa                                                                  <  1:2344681/68‑1 (MQ=255)       aCGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACAcc                                                                     <  1:3513508/65‑1 (MQ=255)       aCGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGAttt                                                               <  1:3367157/71‑1 (MQ=255)        cgcgACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTa                                                              <  1:3746778/71‑1 (MQ=255)        cgcgACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTa                                                              >  1:1437964/1‑71 (MQ=255)            aCGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACgg                                                           >  1:3381941/1‑70 (MQ=255)             cGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGc                                                          >  1:2347202/1‑70 (MQ=255)              ggTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCaa                                                        >  1:277157/1‑71 (MQ=255)                tAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACg                                                      <  1:3363619/71‑1 (MQ=255)                  gTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCg                                                      >  1:1254477/1‑69 (MQ=255)                   tCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGAtt                                                                <  1:2711657/58‑1 (MQ=255)                   tCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTAc                                                             <  1:3535560/61‑1 (MQ=255)                   tCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAg                                                   <  1:2118094/71‑1 (MQ=255)                      ccGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGAt                                                <  1:1036060/71‑1 (MQ=255)                       cGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAg                                                   >  1:762822/1‑67 (MQ=255)                       cGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAg                                                   >  1:2603756/1‑67 (MQ=255)                       cGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATg                                               >  1:1591625/1‑71 (MQ=255)                       cGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGAtt                                                                <  1:1316290/54‑1 (MQ=255)                        gatgatACGGCCACGGCCATATGGATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGt                                              >  1:3618208/1‑71 (MQ=255)                        gatgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGt                                              >  1:2490979/1‑71 (MQ=255)                        gataatGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCAtat                                              <  1:3795941/71‑3 (MQ=255)                         atgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGc                                                     >  1:563614/1‑63 (MQ=255)                         atgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGc                                                     >  1:1713932/1‑63 (MQ=255)                         atgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTc                                             >  1:8738/1‑71 (MQ=255)                         atgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAAGAtag                                              >  1:1777735/1‑68 (MQ=255)                            atACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCgg                                          <  1:2110042/71‑1 (MQ=255)                            atACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCg                                           <  1:264934/70‑1 (MQ=255)                             tACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCg                                           >  1:403082/1‑69 (MQ=255)                             tACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCg                                           >  1:1819363/1‑69 (MQ=255)                                ggccacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAg                                        <  1:3154099/69‑1 (MQ=255)                                 gccacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCa                                                    <  1:681140/56‑1 (MQ=255)                                 cccacggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAgc                                                  >  1:2368655/2‑57 (MQ=255)                                  ccacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAgtgt                                     >  1:2180042/1‑70 (MQ=255)                                  ccacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAgt                                       >  1:2812195/1‑68 (MQ=255)                                   cacggccaTATGCATGTGGCAGACCGGTCAGAACACCAGAtt                                                                <  1:1626566/42‑1 (MQ=255)                                   cacggccaTATGCATCTGTCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaa                                   <  1:5535/71‑1 (MQ=255)                                   cacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGAtt                                                                <  1:3645305/42‑1 (MQ=255)                                   cacggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaa                                   <  1:3006292/71‑1 (MQ=255)                                    acggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                                  >  1:1205796/1‑71 (MQ=255)                                     cggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa                                    >  1:2006983/1‑68 (MQ=255)                                     cggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa                                    <  1:1759126/68‑1 (MQ=255)                                     cggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                                  >  1:3015164/1‑70 (MQ=255)                                     cggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                                  >  1:2389073/1‑70 (MQ=255)                                     cggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                                  >  1:2683014/1‑70 (MQ=255)                                     cggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                                  >  1:2393813/1‑70 (MQ=255)                                      tgccaGATGCATATGGCAGACAGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACg                                >  1:2894211/2‑71 (MQ=255)                                      ggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACg                                >  1:96175/1‑71 (MQ=255)                                      ggccaTATGCATCTGGCAGACAGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACg                                <  1:1154638/71‑1 (MQ=255)                                        ccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAAcat                               >  1:3254109/1‑68 (MQ=255)                                           tatGCATCTGGCAGACCGGTCAGAACACCAGATTTACgg                                                           <  1:735503/39‑1 (MQ=255)                                           tatGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAg                                                   <  1:1244351/47‑1 (MQ=255)                                            atGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGa                                         >  1:3667396/1‑56 (MQ=255)                                            atGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGa                                         >  1:2681569/1‑56 (MQ=255)                                             tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaaa                                  >  1:89121/1‑62 (MQ=255)                                             tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca                         <  1:3713503/71‑1 (MQ=255)                                             tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca                         <  1:1557305/71‑1 (MQ=255)                                             tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca                         <  1:3863764/71‑1 (MQ=255)                                              gCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGa                                         >  1:2082483/1‑54 (MQ=255)                                              ccATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCggt                                         >  1:1971649/2‑53 (MQ=16)                                               cATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACg                       <  1:3294142/71‑1 (MQ=255)                                               cATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaa                                   >  1:3389602/1‑59 (MQ=255)                                               cATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaa                                   >  1:2722572/1‑59 (MQ=255)                                                aTCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATcaa                            <  1:2573065/65‑3 (MQ=255)                                                aTCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGc                      >  1:934271/1‑71 (MQ=255)                                                aTCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAg                                        >  1:84688/1‑53 (MQ=255)                                                aTCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGa                            >  1:622401/1‑65 (MQ=255)                                                aTCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGa                            >  1:3472631/1‑65 (MQ=255)                                                 tCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAg                                                   >  1:2598261/1‑41 (MQ=19)                                                 tCTGGCAGACCGGTCAGAACACCAGATTTACGGCa                                                         <  1:398088/35‑1 (MQ=255)                                                  cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaaa                                  >  1:75521/1‑57 (MQ=255)                                                  cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaaa                                  >  1:430826/1‑57 (MQ=255)                                                  cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                    >  1:683712/1‑71 (MQ=255)                                                  cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                    >  1:1200076/1‑71 (MQ=255)                                                  cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                    >  1:3124160/1‑71 (MQ=255)                                                  cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                    >  1:3202735/1‑71 (MQ=255)                                                    ggCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGa                            <  1:3232434/61‑1 (MQ=255)                                                     gCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGa                            <  1:2694772/60‑1 (MQ=255)                                                       aGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa                                    <  1:1952353/50‑1 (MQ=255)                                                        aaCCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGtta              >  1:3413941/2‑70 (MQ=255)                                                         aCCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGaa                           <  1:540759/57‑1 (MQ=255)                                                         aCCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTtgt             <  1:794064/71‑1 (MQ=255)                                                         aCCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTtgt             >  1:1962760/1‑71 (MQ=255)                                                          ccGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAAc                                 >  1:3332771/1‑50 (MQ=255)                                                          ccGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTtgtg            <  1:1080095/71‑1 (MQ=255)                                                           cGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGa           <  1:472274/71‑1 (MQ=255)                                                            ggTCAGAACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCACTGGTtatggg          >  1:2884392/1‑69 (MQ=255)                                                             gTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGa           >  1:2894459/1‑69 (MQ=255)                                                              tCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGGCGAACACGCCCTGGTTGTGAGtt        <  1:1754112/71‑1 (MQ=255)                                                               cAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGttt       >  1:10591/1‑71 (MQ=255)                                                                aGAACTCCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGtttt      <  1:2585645/71‑1 (MQ=255)                                                                aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTTTAAACGTCGAACACGCCCTGGGTGTGAGtttt      <  1:3339867/71‑1 (MQ=255)                                                                aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCg                             <  1:3287562/48‑1 (MQ=255)                                                                aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGttt       <  1:2490538/70‑1 (MQ=255)                                                                aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGttt       <  1:858471/70‑1 (MQ=255)                                                                aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGttt       <  1:186373/70‑1 (MQ=255)                                                                  aaCACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTa     <  1:816445/70‑1 (MQ=255)                                                                    cacCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACgg  >  1:437384/1‑71 (MQ=255)                                                                    cacCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACgg  >  1:1129451/1‑71 (MQ=255)                                                                     |                                                                      AGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGG  >  minE/609694‑609831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑