BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	609,783	G→C	19.2%	I157M (ATC→ATG)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	609,783	0	G	C	19.2%	214.2 / 44.0	99	I157M (ATC→ATG)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base G (47/33); new base C (14/5); total (61/38)
Fisher's exact test for biased strand distribution p-value = 2.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.19e-01

Only 100 of 103 total aligned reads displayed.

CCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGA  >  minE/609715‑609853

ccGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGAt                                                                      <  1:1036060/71‑1 (MQ=255)

 cGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATg                                                                     >  1:1591625/1‑71 (MQ=255)

  gatgatACGGCCACGGCCATATGGATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGt                                                                    >  1:3618208/1‑71 (MQ=255)

  gatgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGt                                                                    >  1:2490979/1‑71 (MQ=255)

  gataatGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCAtat                                                                    <  1:3795941/71‑3 (MQ=255)

   atgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTc                                                                   >  1:8738/1‑71 (MQ=255)

   atgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAAGAtag                                                                    >  1:1777735/1‑68 (MQ=255)

      atACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCgg                                                                <  1:2110042/71‑1 (MQ=255)

      atACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCg                                                                 <  1:264934/70‑1 (MQ=255)

       tACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCg                                                                 >  1:403082/1‑69 (MQ=255)

       tACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCg                                                                 >  1:1819363/1‑69 (MQ=255)

          ggccacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAg                                                              <  1:3154099/69‑1 (MQ=255)

            ccacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAgtgt                                                           >  1:2180042/1‑70 (MQ=255)

            ccacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAgt                                                             >  1:2812195/1‑68 (MQ=255)

             cacggccaTATGCATCTGTCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaa                                                         <  1:5535/71‑1 (MQ=255)

             cacggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaa                                                         <  1:3006292/71‑1 (MQ=255)

              acggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                                                        >  1:1205796/1‑71 (MQ=255)

               cggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa                                                          >  1:2006983/1‑68 (MQ=255)

               cggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa                                                          <  1:1759126/68‑1 (MQ=255)

               cggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                                                        >  1:2393813/1‑70 (MQ=255)

               cggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                                                        >  1:2389073/1‑70 (MQ=255)

               cggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                                                        >  1:2683014/1‑70 (MQ=255)

               cggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                                                        >  1:3015164/1‑70 (MQ=255)

                tgccaGATGCATATGGCAGACAGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACg                                                      >  1:2894211/2‑71 (MQ=255)

                ggccaTATGCATCTGGCAGACAGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACg                                                      <  1:1154638/71‑1 (MQ=255)

                      atGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGa                                                               >  1:2681569/1‑56 (MQ=255)

                      atGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGa                                                               >  1:3667396/1‑56 (MQ=255)

                       tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaaa                                                        >  1:89121/1‑62 (MQ=255)

                       tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca                                               <  1:1557305/71‑1 (MQ=255)

                       tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca                                               <  1:3863764/71‑1 (MQ=255)

                       tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca                                               <  1:3713503/71‑1 (MQ=255)

                        gCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGa                                                               >  1:2082483/1‑54 (MQ=255)

                        ccATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCggt                                                               >  1:1971649/2‑53 (MQ=16)

                         cATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACg                                             <  1:3294142/71‑1 (MQ=255)

                         cATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaa                                                         >  1:2722572/1‑59 (MQ=255)

                         cATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaa                                                         >  1:3389602/1‑59 (MQ=255)

                          aTCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATcaa                                                  <  1:2573065/65‑3 (MQ=255)

                          aTCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGc                                            >  1:934271/1‑71 (MQ=255)

                          aTCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAg                                                              >  1:84688/1‑53 (MQ=255)

                          aTCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGa                                                  >  1:3472631/1‑65 (MQ=255)

                          aTCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGa                                                  >  1:622401/1‑65 (MQ=255)

                            cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaaa                                                        >  1:430826/1‑57 (MQ=255)

                            cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaaa                                                        >  1:75521/1‑57 (MQ=255)

                            cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                                          >  1:3202735/1‑71 (MQ=255)

                            cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                                          >  1:683712/1‑71 (MQ=255)

                            cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                                          >  1:3124160/1‑71 (MQ=255)

                            cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                                          >  1:1200076/1‑71 (MQ=255)

                              ggCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGa                                                  <  1:3232434/61‑1 (MQ=255)

                               gCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGa                                                  <  1:2694772/60‑1 (MQ=255)

                                 aGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa                                                          <  1:1952353/50‑1 (MQ=255)

                                  aaCCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGtta                                    >  1:3413941/2‑70 (MQ=255)

                                   aCCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGaa                                                 <  1:540759/57‑1 (MQ=255)

                                   aCCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTtgt                                   <  1:794064/71‑1 (MQ=255)

                                   aCCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTtgt                                   >  1:1962760/1‑71 (MQ=255)

                                    ccGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAAc                                                       >  1:3332771/1‑50 (MQ=255)

                                    ccGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTtgtg                                  <  1:1080095/71‑1 (MQ=255)

                                     cGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGa                                 <  1:472274/71‑1 (MQ=255)

                                      ggTCAGAACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCACTGGTtatggg                                >  1:2884392/1‑69 (MQ=255)

                                       gTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGa                                 >  1:2894459/1‑69 (MQ=255)

                                        tCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGGCGAACACGCCCTGGTTGTGAGtt                              <  1:1754112/71‑1 (MQ=255)

                                         cAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGttt                             >  1:10591/1‑71 (MQ=255)

                                          aGAACTCCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGtttt                            <  1:2585645/71‑1 (MQ=255)

                                          aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTTTAAACGTCGAACACGCCCTGGGTGTGAGtttt                            <  1:3339867/71‑1 (MQ=255)

                                          aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCg                                                   <  1:3287562/48‑1 (MQ=255)

                                          aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGttt                             <  1:858471/70‑1 (MQ=255)

                                          aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGttt                             <  1:186373/70‑1 (MQ=255)

                                          aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGttt                             <  1:2490538/70‑1 (MQ=255)

                                            aaCACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTa                           <  1:816445/70‑1 (MQ=255)

                                             acgccAGATTTACGGCATCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGTTTTACg                         >  1:1815777/4‑71 (MQ=255)

                                              cacCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACgg                        >  1:1129451/1‑71 (MQ=255)

                                              cacCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACgg                        >  1:437384/1‑71 (MQ=255)

                                               gccAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGttt                             <  1:1631026/64‑1 (MQ=255)

                                               gccAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGTTTTACg                         >  1:2061190/2‑69 (MQ=255)

                                                 cAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTAt                     >  1:1531336/1‑71 (MQ=255)

                                                  aGATTTACGGCAGCGCAGCATATCCGGTGAGTAAAc                                                       >  1:3579797/1‑36 (MQ=12)

                                                  aGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGtt                                     >  1:1452631/1‑54 (MQ=255)

                                                   gATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGTTTTACg                         >  1:1326924/1‑65 (MQ=255)

                                                   gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACg                                             >  1:1028313/1‑45 (MQ=255)

                                                   gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGt                       >  1:1099139/1‑67 (MQ=255)

                                                   gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTAtt                    >  1:593872/1‑70 (MQ=255)

                                                   gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTc                   <  1:1668607/71‑1 (MQ=255)

                                                      ttACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTg                                        >  1:2056657/1‑47 (MQ=255)

                                                      ttACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGt                >  1:3031529/1‑71 (MQ=255)

                                                      ttACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGt                >  1:1708068/1‑71 (MQ=255)

                                                       tACGGCAACGCAGGATGTCCGGAGTGTAAACGTCg                                                   <  1:627472/35‑1 (MQ=255)

                                                       tACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGt                <  1:2080340/70‑1 (MQ=255)

                                                        aCGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTg               <  1:2314118/70‑1 (MQ=255)

                                                        aCGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTg               <  1:3194530/70‑1 (MQ=255)

                                                         cGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGa              <  1:1607697/70‑1 (MQ=255)

                                                             aaCGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAtt         >  1:1050678/1‑71 (MQ=255)

                                                              aCGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                                          >  1:1502410/1‑37 (MQ=255)

                                                              aCGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                                          >  1:649819/1‑37 (MQ=255)

                                                              aCGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGa           >  1:3117557/1‑68 (MQ=255)

                                                                gCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAg                                <  1:82200/45‑1 (MQ=255)

                                                                gCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAttttt      <  1:1930890/71‑1 (MQ=255)

                                                                 cAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAtttttt     >  1:1831887/1‑71 (MQ=255)

                                                                  aGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAttttttt    >  1:1797344/1‑71 (MQ=255)

                                                                   ggATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTg   <  1:1372368/71‑1 (MQ=255)

                                                                    gATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGa  <  1:2937464/71‑1 (MQ=255)

                                                                    gATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGa  <  1:2074140/71‑1 (MQ=255)

CCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGA  >  minE/609715‑609853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`