BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	609,786	G→A	17.6%	D156D (GAC→GAT)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	609,786	0	G	A	17.6%	239.5 / 38.3	102	D156D (GAC→GAT)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base G (47/37); new base A (14/4); total (61/41)
Fisher's exact test for biased strand distribution p-value = 1.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 103 total aligned reads displayed.

CGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATC  >  minE/609716‑609855

cGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATg                                                                       >  1:1591625/1‑71 (MQ=255)

 gatgatACGGCCACGGCCATATGGATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGt                                                                      >  1:3618208/1‑71 (MQ=255)

 gatgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGt                                                                      >  1:2490979/1‑71 (MQ=255)

  atgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTc                                                                     >  1:8738/1‑71 (MQ=255)

     atACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCgg                                                                  <  1:2110042/71‑1 (MQ=255)

     atACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCg                                                                   <  1:264934/70‑1 (MQ=255)

      tACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCg                                                                   >  1:1819363/1‑69 (MQ=255)

      tACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCg                                                                   >  1:403082/1‑69 (MQ=255)

         ggccacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAg                                                                <  1:3154099/69‑1 (MQ=255)

           ccacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAgtgt                                                             >  1:2180042/1‑70 (MQ=255)

           ccacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAgt                                                               >  1:2812195/1‑68 (MQ=255)

            cacggccaTATGCATCTGTCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaa                                                           <  1:5535/71‑1 (MQ=255)

            cacggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaa                                                           <  1:3006292/71‑1 (MQ=255)

             acggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                                                          >  1:1205796/1‑71 (MQ=255)

              cggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa                                                            >  1:2006983/1‑68 (MQ=255)

              cggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa                                                            <  1:1759126/68‑1 (MQ=255)

              cggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                                                          >  1:3015164/1‑70 (MQ=255)

              cggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                                                          >  1:2389073/1‑70 (MQ=255)

              cggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                                                          >  1:2393813/1‑70 (MQ=255)

              cggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                                                          >  1:2683014/1‑70 (MQ=255)

               tgccaGATGCATATGGCAGACAGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACg                                                        >  1:2894211/2‑71 (MQ=255)

               ggccaTATGCATCTGGCAGACAGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACg                                                        <  1:1154638/71‑1 (MQ=255)

                     atGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGa                                                                 >  1:3667396/1‑56 (MQ=255)

                     atGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGa                                                                 >  1:2681569/1‑56 (MQ=255)

                      tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaaa                                                          >  1:89121/1‑62 (MQ=255)

                      tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca                                                 <  1:3713503/71‑1 (MQ=255)

                      tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca                                                 <  1:3863764/71‑1 (MQ=255)

                      tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca                                                 <  1:1557305/71‑1 (MQ=255)

                       gCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGa                                                                 >  1:2082483/1‑54 (MQ=255)

                       ccATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCggt                                                                 >  1:1971649/2‑53 (MQ=16)

                        cATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACg                                               <  1:3294142/71‑1 (MQ=255)

                        cATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaa                                                           >  1:3389602/1‑59 (MQ=255)

                        cATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaa                                                           >  1:2722572/1‑59 (MQ=255)

                         aTCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATcaa                                                    <  1:2573065/65‑3 (MQ=255)

                         aTCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGc                                              >  1:934271/1‑71 (MQ=255)

                         aTCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAg                                                                >  1:84688/1‑53 (MQ=255)

                         aTCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGa                                                    >  1:3472631/1‑65 (MQ=255)

                         aTCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGa                                                    >  1:622401/1‑65 (MQ=255)

                           cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaaa                                                          >  1:430826/1‑57 (MQ=255)

                           cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaaa                                                          >  1:75521/1‑57 (MQ=255)

                           cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                                            >  1:3124160/1‑71 (MQ=255)

                           cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                                            >  1:3202735/1‑71 (MQ=255)

                           cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                                            >  1:683712/1‑71 (MQ=255)

                           cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                                            >  1:1200076/1‑71 (MQ=255)

                             ggCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGa                                                    <  1:3232434/61‑1 (MQ=255)

                              gCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGa                                                    <  1:2694772/60‑1 (MQ=255)

                                aGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa                                                            <  1:1952353/50‑1 (MQ=255)

                                 aaCCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGtta                                      >  1:3413941/2‑70 (MQ=255)

                                  aCCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGaa                                                   <  1:540759/57‑1 (MQ=255)

                                  aCCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTtgt                                     <  1:794064/71‑1 (MQ=255)

                                  aCCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTtgt                                     >  1:1962760/1‑71 (MQ=255)

                                   ccGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAAc                                                         >  1:3332771/1‑50 (MQ=255)

                                   ccGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTtgtg                                    <  1:1080095/71‑1 (MQ=255)

                                    cGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGa                                   <  1:472274/71‑1 (MQ=255)

                                     ggTCAGAACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCACTGGTtatggg                                  >  1:2884392/1‑69 (MQ=255)

                                      gTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGa                                   >  1:2894459/1‑69 (MQ=255)

                                       tCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGGCGAACACGCCCTGGTTGTGAGtt                                <  1:1754112/71‑1 (MQ=255)

                                        cAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGttt                               >  1:10591/1‑71 (MQ=255)

                                         aGAACTCCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGtttt                              <  1:2585645/71‑1 (MQ=255)

                                         aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTTTAAACGTCGAACACGCCCTGGGTGTGAGtttt                              <  1:3339867/71‑1 (MQ=255)

                                         aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCg                                                     <  1:3287562/48‑1 (MQ=255)

                                         aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGttt                               <  1:186373/70‑1 (MQ=255)

                                         aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGttt                               <  1:858471/70‑1 (MQ=255)

                                         aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGttt                               <  1:2490538/70‑1 (MQ=255)

                                           aaCACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTa                             <  1:816445/70‑1 (MQ=255)

                                            acgccAGATTTACGGCATCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGTTTTACg                           >  1:1815777/4‑71 (MQ=255)

                                             cacCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACgg                          >  1:1129451/1‑71 (MQ=255)

                                             cacCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACgg                          >  1:437384/1‑71 (MQ=255)

                                              gccAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGttt                               <  1:1631026/64‑1 (MQ=255)

                                              gccAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGTTTTACg                           >  1:2061190/2‑69 (MQ=255)

                                                cAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTAt                       >  1:1531336/1‑71 (MQ=255)

                                                 aGATTTACGGCAGCGCAGCATATCCGGTGAGTAAAc                                                         >  1:3579797/1‑36 (MQ=12)

                                                 aGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGtt                                       >  1:1452631/1‑54 (MQ=255)

                                                  gATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGTTTTACg                           >  1:1326924/1‑65 (MQ=255)

                                                  gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACg                                               >  1:1028313/1‑45 (MQ=255)

                                                  gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGt                         >  1:1099139/1‑67 (MQ=255)

                                                  gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTAtt                      >  1:593872/1‑70 (MQ=255)

                                                  gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTc                     <  1:1668607/71‑1 (MQ=255)

                                                     ttACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGt                  >  1:1708068/1‑71 (MQ=255)

                                                     ttACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGt                  >  1:3031529/1‑71 (MQ=255)

                                                      tACGGCAACGCAGGATGTCCGGAGTGTAAACGTCg                                                     <  1:627472/35‑1 (MQ=255)

                                                      tACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGt                  <  1:2080340/70‑1 (MQ=255)

                                                       aCGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTg                 <  1:2314118/70‑1 (MQ=255)

                                                       aCGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTg                 <  1:3194530/70‑1 (MQ=255)

                                                        cGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGa                <  1:1607697/70‑1 (MQ=255)

                                                            aaCGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAtt           >  1:1050678/1‑71 (MQ=255)

                                                             aCGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                                            >  1:649819/1‑37 (MQ=255)

                                                             aCGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                                            >  1:1502410/1‑37 (MQ=255)

                                                             aCGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGa             >  1:3117557/1‑68 (MQ=255)

                                                               gCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAg                                  <  1:82200/45‑1 (MQ=255)

                                                               gCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAttttt        <  1:1930890/71‑1 (MQ=255)

                                                                cAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAtttttt       >  1:1831887/1‑71 (MQ=255)

                                                                 aGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAttttttt      >  1:1797344/1‑71 (MQ=255)

                                                                  ggATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTg     <  1:1372368/71‑1 (MQ=255)

                                                                   gATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGa    <  1:2937464/71‑1 (MQ=255)

                                                                   gATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGa    <  1:2008984/71‑1 (MQ=255)

                                                                   gATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGa    <  1:2074140/71‑1 (MQ=255)

                                                                    aTGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGat   >  1:3690858/1‑71 (MQ=255)

                                                                     tGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGatc  <  1:1569361/71‑1 (MQ=255)

                                                                     tGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGat   >  1:2696638/1‑70 (MQ=255)

CGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATC  >  minE/609716‑609855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`