BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	609,792	A→T	16.8%	T154T (ACT→ACA) ‡	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	609,792	0	A	T	16.8%	253.2 / 39.2	101	T154T (ACT→ACA) ‡	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base A (41/43); new base T (13/4); total (54/47)
Fisher's exact test for biased strand distribution p-value = 6.00e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

GGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCGGA  >  minE/609725‑609861

ggccacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAg                                                                      <  1:3154099/69‑1 (MQ=255)

  ccacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAgtgt                                                                   >  1:2180042/1‑70 (MQ=255)

  ccacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAgt                                                                     >  1:2812195/1‑68 (MQ=255)

   cacggccaTATGCATCTGTCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaa                                                                 <  1:5535/71‑1 (MQ=255)

   cacggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaa                                                                 <  1:3006292/71‑1 (MQ=255)

    acggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                                                                >  1:1205796/1‑71 (MQ=255)

     cggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa                                                                  >  1:2006983/1‑68 (MQ=255)

     cggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa                                                                  <  1:1759126/68‑1 (MQ=255)

     cggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                                                                >  1:3015164/1‑70 (MQ=255)

     cggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                                                                >  1:2683014/1‑70 (MQ=255)

     cggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                                                                >  1:2393813/1‑70 (MQ=255)

     cggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                                                                >  1:2389073/1‑70 (MQ=255)

      tgccaGATGCATATGGCAGACAGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACg                                                              >  1:2894211/2‑71 (MQ=255)

      ggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACg                                                              >  1:96175/1‑71 (MQ=255)

      ggccaTATGCATCTGGCAGACAGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACg                                                              <  1:1154638/71‑1 (MQ=255)

        ccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAAcat                                                             >  1:3254109/1‑68 (MQ=255)

            atGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGa                                                                       >  1:2681569/1‑56 (MQ=255)

             tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaaa                                                                >  1:89121/1‑62 (MQ=255)

             tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca                                                       <  1:1557305/71‑1 (MQ=255)

             tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca                                                       <  1:3863764/71‑1 (MQ=255)

             tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca                                                       <  1:3713503/71‑1 (MQ=255)

              gCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGa                                                                       >  1:2082483/1‑54 (MQ=255)

               cATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACg                                                     <  1:3294142/71‑1 (MQ=255)

               cATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaa                                                                 >  1:3389602/1‑59 (MQ=255)

               cATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaa                                                                 >  1:2722572/1‑59 (MQ=255)

                aTCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATcaa                                                          <  1:2573065/65‑3 (MQ=255)

                aTCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGc                                                    >  1:934271/1‑71 (MQ=255)

                aTCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAg                                                                      >  1:84688/1‑53 (MQ=255)

                aTCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGa                                                          >  1:3472631/1‑65 (MQ=255)

                aTCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGa                                                          >  1:622401/1‑65 (MQ=255)

                  cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaaa                                                                >  1:75521/1‑57 (MQ=255)

                  cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaaa                                                                >  1:430826/1‑57 (MQ=255)

                  cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                                                  >  1:3124160/1‑71 (MQ=255)

                  cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                                                  >  1:3202735/1‑71 (MQ=255)

                  cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                                                  >  1:1200076/1‑71 (MQ=255)

                  cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                                                  >  1:683712/1‑71 (MQ=255)

                    ggCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGa                                                          <  1:3232434/61‑1 (MQ=255)

                     gCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGa                                                          <  1:2694772/60‑1 (MQ=255)

                       aGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa                                                                  <  1:1952353/50‑1 (MQ=255)

                        aaCCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGtta                                            >  1:3413941/2‑70 (MQ=255)

                         aCCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGaa                                                         <  1:540759/57‑1 (MQ=255)

                         aCCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTtgt                                           >  1:1962760/1‑71 (MQ=255)

                         aCCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTtgt                                           <  1:794064/71‑1 (MQ=255)

                          ccGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAAc                                                               >  1:3332771/1‑50 (MQ=255)

                          ccGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTtgtg                                          <  1:1080095/71‑1 (MQ=255)

                           cGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGa                                         <  1:472274/71‑1 (MQ=255)

                             gTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGa                                         >  1:2894459/1‑69 (MQ=255)

                              tCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGGCGAACACGCCCTGGTTGTGAGtt                                      <  1:1754112/71‑1 (MQ=255)

                               cAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGttt                                     >  1:10591/1‑71 (MQ=255)

                                aGAACTCCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGtttt                                    <  1:2585645/71‑1 (MQ=255)

                                aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTTTAAACGTCGAACACGCCCTGGGTGTGAGtttt                                    <  1:3339867/71‑1 (MQ=255)

                                aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCg                                                           <  1:3287562/48‑1 (MQ=255)

                                aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGttt                                     <  1:186373/70‑1 (MQ=255)

                                aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGttt                                     <  1:2490538/70‑1 (MQ=255)

                                aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGttt                                     <  1:858471/70‑1 (MQ=255)

                                  aaCACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTa                                   <  1:816445/70‑1 (MQ=255)

                                    cacCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACgg                                >  1:1129451/1‑71 (MQ=255)

                                    cacCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACgg                                >  1:437384/1‑71 (MQ=255)

                                     gccAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGttt                                     <  1:1631026/64‑1 (MQ=255)

                                     gccAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGTTTTACg                                 >  1:2061190/2‑69 (MQ=255)

                                       cAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTAt                             >  1:1531336/1‑71 (MQ=255)

                                        aGATTTACGGCAGCGCAGCATATCCGGTGAGTAAAc                                                               >  1:3579797/1‑36 (MQ=12)

                                        aGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGtt                                             >  1:1452631/1‑54 (MQ=255)

                                         gATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGTTTTACg                                 >  1:1326924/1‑65 (MQ=255)

                                         gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACg                                                     >  1:1028313/1‑45 (MQ=255)

                                         gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGt                               >  1:1099139/1‑67 (MQ=255)

                                         gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTAtt                            >  1:593872/1‑70 (MQ=255)

                                         gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTc                           <  1:1668607/71‑1 (MQ=255)

                                            ttACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTg                                                >  1:2056657/1‑47 (MQ=255)

                                            ttACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGt                        >  1:3031529/1‑71 (MQ=255)

                                            ttACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGt                        >  1:1708068/1‑71 (MQ=255)

                                             tACGGCAACGCAGGATGTCCGGAGTGTAAACGTCg                                                           <  1:627472/35‑1 (MQ=255)

                                             tACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGt                        <  1:2080340/70‑1 (MQ=255)

                                              aCGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTg                       <  1:3194530/70‑1 (MQ=255)

                                              aCGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTg                       <  1:2314118/70‑1 (MQ=255)

                                               cGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGa                      <  1:1607697/70‑1 (MQ=255)

                                                   aaCGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAtt                 >  1:1050678/1‑71 (MQ=255)

                                                    aCGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                                                  >  1:1502410/1‑37 (MQ=255)

                                                    aCGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                                                  >  1:649819/1‑37 (MQ=255)

                                                    aCGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGa                   >  1:3117557/1‑68 (MQ=255)

                                                      gCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAg                                        <  1:82200/45‑1 (MQ=255)

                                                      gCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAttttt              <  1:1930890/71‑1 (MQ=255)

                                                       cAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAtttttt             >  1:1831887/1‑71 (MQ=255)

                                                        aGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAttttttt            >  1:1797344/1‑71 (MQ=255)

                                                         ggATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTg           <  1:1372368/71‑1 (MQ=255)

                                                          gATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGa          <  1:2074140/71‑1 (MQ=255)

                                                          gATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGa          <  1:2008984/71‑1 (MQ=255)

                                                          gATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGa          <  1:2937464/71‑1 (MQ=255)

                                                           aTGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGat         >  1:3690858/1‑71 (MQ=255)

                                                            tGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGatc        <  1:1569361/71‑1 (MQ=255)

                                                            tGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGat         >  1:2696638/1‑70 (MQ=255)

                                                               ccGGAGTGTAAACGTCTAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGt                        <  1:1123984/52‑1 (MQ=255)

                                                                cGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGatcatc     <  1:392250/70‑1 (MQ=255)

                                                                cGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGatcatc     <  1:3007321/70‑1 (MQ=255)

                                                                cGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCg    >  1:622611/1‑71 (MQ=255)

                                                                cGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCg    <  1:418031/71‑1 (MQ=255)

                                                                 ggAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCgg   <  1:351944/71‑1 (MQ=255)

                                                                 ggAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCgg   <  1:2932642/71‑1 (MQ=255)

                                                                 ggAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCgg   <  1:3602155/71‑1 (MQ=255)

                                                                  gAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAg                         <  1:1069834/48‑1 (MQ=255)

GGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCGGA  >  minE/609725‑609861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`