Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	609,807	C→T	9.7%	V149V (GTG→GTA)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	609,807	0	C	T	9.7%	233.9 / 11.2	82	V149V (GTG→GTA)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base C (37/37);  new base T (6/2);  total (43/39)
Fisher's exact test for biased strand distribution p-value = 2.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

TGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCGGATCCAGTTCGCGGTTG  >  minE/609738‑609876                                                                      |                                                                      tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca                                                                      <  1:3713503/71‑1 (MQ=255) tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca                                                                      <  1:3863764/71‑1 (MQ=255) tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca                                                                      <  1:1557305/71‑1 (MQ=255)   cATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACg                                                                    <  1:3294142/71‑1 (MQ=255)    aTCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGc                                                                   >  1:934271/1‑71 (MQ=255)      cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                                                                 >  1:3124160/1‑71 (MQ=255)      cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                                                                 >  1:683712/1‑71 (MQ=255)      cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                                                                 >  1:1200076/1‑71 (MQ=255)      cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                                                                 >  1:3202735/1‑71 (MQ=255)            aaCCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGtta                                                           >  1:3413941/2‑70 (MQ=255)             aCCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTtgt                                                          >  1:1962760/1‑71 (MQ=255)             aCCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTtgt                                                          <  1:794064/71‑1 (MQ=255)              ccGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTtgtg                                                         <  1:1080095/71‑1 (MQ=255)               cGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGa                                                        <  1:472274/71‑1 (MQ=255)                ggTCAGAACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCACTGGTtatggg                                                       >  1:2884392/1‑69 (MQ=255)                 gTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGa                                                        >  1:2894459/1‑69 (MQ=255)                  tCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGGCGAACACGCCCTGGTTGTGAGtt                                                     <  1:1754112/71‑1 (MQ=255)                   cAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGttt                                                    >  1:10591/1‑71 (MQ=255)                    aGAACTCCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGtttt                                                   <  1:2585645/71‑1 (MQ=255)                    aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTTTAAACGTCGAACACGCCCTGGGTGTGAGtttt                                                   <  1:3339867/71‑1 (MQ=255)                    aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGttt                                                    <  1:858471/70‑1 (MQ=255)                    aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGttt                                                    <  1:2490538/70‑1 (MQ=255)                    aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGttt                                                    <  1:186373/70‑1 (MQ=255)                      aaCACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTa                                                  <  1:816445/70‑1 (MQ=255)                       acgccAGATTTACGGCATCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGTTTTACg                                                >  1:1815777/4‑71 (MQ=255)                        cacCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACgg                                               >  1:437384/1‑71 (MQ=255)                        cacCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACgg                                               >  1:1129451/1‑71 (MQ=255)                         gccAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGttt                                                    <  1:1631026/64‑1 (MQ=255)                         gccAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGTTTTACg                                                >  1:2061190/2‑69 (MQ=255)                           cAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTAt                                            >  1:1531336/1‑71 (MQ=255)                            aGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGtt                                                            >  1:1452631/1‑54 (MQ=255)                             gATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGTTTTACg                                                >  1:1326924/1‑65 (MQ=255)                             gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACg                                                                    >  1:1028313/1‑45 (MQ=255)                             gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGt                                              >  1:1099139/1‑67 (MQ=255)                             gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTAtt                                           >  1:593872/1‑70 (MQ=255)                             gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTc                                          <  1:1668607/71‑1 (MQ=255)                                ttACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTg                                                               >  1:2056657/1‑47 (MQ=255)                                ttACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGt                                       >  1:3031529/1‑71 (MQ=255)                                ttACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGt                                       >  1:1708068/1‑71 (MQ=255)                                 tACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGt                                       <  1:2080340/70‑1 (MQ=255)                                  aCGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTg                                      <  1:2314118/70‑1 (MQ=255)                                  aCGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTg                                      <  1:3194530/70‑1 (MQ=255)                                   cGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGa                                     <  1:1607697/70‑1 (MQ=255)                                       aaCGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAtt                                >  1:1050678/1‑71 (MQ=255)                                        aCGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                                                                 >  1:649819/1‑37 (MQ=255)                                        aCGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                                                                 >  1:1502410/1‑37 (MQ=255)                                        aCGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGa                                  >  1:3117557/1‑68 (MQ=255)                                          gCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAg                                                       <  1:82200/45‑1 (MQ=255)                                          gCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAttttt                             <  1:1930890/71‑1 (MQ=255)                                           cAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAtttttt                            >  1:1831887/1‑71 (MQ=255)                                            aGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAttttttt                           >  1:1797344/1‑71 (MQ=255)                                             ggATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTg                          <  1:1372368/71‑1 (MQ=255)                                              gATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGa                         <  1:2074140/71‑1 (MQ=255)                                              gATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGa                         <  1:2008984/71‑1 (MQ=255)                                              gATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGa                         <  1:2937464/71‑1 (MQ=255)                                               aTGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGat                        >  1:3690858/1‑71 (MQ=255)                                                tGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGatc                       <  1:1569361/71‑1 (MQ=255)                                                tGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGat                        >  1:2696638/1‑70 (MQ=255)                                                   ccGGAGTGTAAACGTCTAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGt                                       <  1:1123984/52‑1 (MQ=255)                                                    cGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGatcatc                    <  1:3007321/70‑1 (MQ=255)                                                    cGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGatcatc                    <  1:392250/70‑1 (MQ=255)                                                    cGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCg                   <  1:418031/71‑1 (MQ=255)                                                    cGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCg                   >  1:622611/1‑71 (MQ=255)                                                     ggAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCgg                  <  1:2932642/71‑1 (MQ=255)                                                     ggAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCgg                  <  1:351944/71‑1 (MQ=255)                                                     ggAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCgg                  <  1:3602155/71‑1 (MQ=255)                                                      gAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAg                                        <  1:1069834/48‑1 (MQ=255)                                                      gAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCGGa                 >  1:985011/1‑71 (MQ=255)                                                         tgtAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCGGATcc              <  1:2847058/71‑1 (MQ=255)                                                           tAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAtttt                              <  1:3734233/53‑1 (MQ=255)                                                           tAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCGGATCCAg            >  1:3697829/1‑71 (MQ=255)                                                           tAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCGGATCCAg            >  1:2603706/1‑71 (MQ=255)                                                             aaCGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCGGATCCAGtt          >  1:3641481/1‑71 (MQ=255)                                                              aCGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCGGa                 >  1:3677054/1‑63 (MQ=255)                                                              aCGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCGGATCCAGTTc         >  1:1798891/1‑71 (MQ=255)                                                              aCGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCGGATCCAGTTc         >  1:3148315/1‑71 (MQ=255)                                                               cGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAttttt                             <  1:1078831/50‑1 (MQ=255)                                                               cGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCGGATCCAGTTcg        <  1:3790389/71‑1 (MQ=255)                                                               cGTCGAACACGCCCTGGTTGTGAGATTTACGGTATTCAGTGAAGATTTTTTTGATCATCGGATCCAGTTcg        >  1:1820147/1‑71 (MQ=255)                                                                gTCGAACACGCCCTGGTTGTGAGTTTTAGGGTATTCAGTGAAGATTTTTTTGATCATCGGATCCAGTTcgc       >  1:1263605/1‑71 (MQ=255)                                                                gTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCGGATCCAGTTcgc       >  1:253230/1‑71 (MQ=255)                                                                gTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCGGATCCAGTTcg        >  1:3811134/1‑70 (MQ=255)                                                                   gAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTg                          <  1:664459/49‑1 (MQ=255)                                                                   gAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTg                          <  1:1816992/49‑1 (MQ=255)                                                                   gAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTg                          <  1:1402262/49‑1 (MQ=255)                                                                     acacGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCGGATCCAGTTCGCGGTTg  <  1:2620820/71‑1 (MQ=255)                                                                      cacGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCGGATCCAGTTCGCgg     >  1:1398152/1‑67 (MQ=255)                                                                      |                                                                      TGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCGGATCCAGTTCGCGGTTG  >  minE/609738‑609876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑