| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 684,711 | G→A | 18.7% | D23N (GAC→AAC) | yccU → | predicted CoA‑binding protein with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 684,711 | 0 | G | A | 18.7% | 66.6 / 9.8 | 32 | D23N (GAC→AAC) | yccU | predicted CoA‑binding protein with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base G (14/12); new base A (3/3); total (17/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
ATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCG > minE/684642‑684779 | ataatGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGa > 1:397991/1‑71 (MQ=255) aCCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGa > 1:425407/1‑59 (MQ=255) gATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTcc < 1:88174/71‑1 (MQ=255) gATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCAACAAACCCGATCGTcc < 1:3034960/71‑1 (MQ=255) tGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGa > 1:454807/1‑51 (MQ=255) gCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACa < 1:3495377/52‑1 (MQ=255) gCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCAACAAACCCGATCGTccc > 1:864848/1‑62 (MQ=255) tttAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCAACaaa > 1:420787/1‑46 (MQ=255) aCGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGaa < 1:224133/71‑1 (MQ=255) gTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAat < 1:2899892/71‑1 (MQ=255) tCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAata < 1:3774997/71‑1 (MQ=255) cacaCCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATcgcg > 1:2397122/1‑55 (MQ=255) cacaCCATTGCGCTGGTGGGGGCAAGCAACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACt > 1:945453/1‑71 (MQ=255) acacCATTGCGCTGGTGGGGGCAAGCGACAAACCCGAt > 1:2508580/1‑38 (MQ=255) ccATTGCGCTGGTGGGGGCAAGCAACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGAc < 1:581241/71‑1 (MQ=255) aTTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAatat < 1:1925019/60‑1 (MQ=255) aTTGCGCTGGTGGGGGCAAGCAACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCa < 1:985529/71‑1 (MQ=255) tGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCt > 1:1699369/1‑60 (MQ=255) tGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCt > 1:1058337/1‑60 (MQ=255) cTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCa < 1:1824242/65‑1 (MQ=255) cTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTa < 1:324572/71‑1 (MQ=255) gggCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACg > 1:2476250/1‑69 (MQ=255) gggCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGt < 1:595595/70‑1 (MQ=255) gcaagcaaCAAACCCGATCGTCCCAGCTATCGCGtgatg < 1:1050319/39‑1 (MQ=255) cAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACg < 1:1101572/66‑1 (MQ=255) cAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCAtt < 1:263143/71‑1 (MQ=255) aaGCGACAAACCCGATCGTCCCAGCTATCGCGtgatga < 1:572550/38‑1 (MQ=255) gCGACAAACCCGATCGTCCCAGCTATCGCGTGATGaaa > 1:2087527/1‑38 (MQ=255) gCGACAAACCCGATCGTCCCAGCTATCGCGTGATGaaa > 1:567741/1‑38 (MQ=255) gCGACAAACCCGATCGTCCCAGCTATCGCGTGATGaaa > 1:585505/1‑38 (MQ=255) gCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCg > 1:3608129/1‑71 (MQ=255) gCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCg > 1:839735/1‑71 (MQ=255) gCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCg > 1:1418023/1‑71 (MQ=255) | ATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCG > minE/684642‑684779 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |