| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 705,295 | A→G | 50.7% | G32G (GGT→GGC) | ymcB ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 705,295 | 0 | A | G | 50.7% | 0.4 / 92.0 | 67 | G32G (GGT→GGC) | ymcB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (15/18); new base G (12/22); total (27/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGC > minE/705226‑705359 | gcgcAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCAc < 1:1539634/71‑1 (MQ=255) cgcAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGcc < 1:2356304/71‑1 (MQ=255) gcAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCa < 1:2452920/71‑1 (MQ=255) gCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCa > 1:2645671/1‑62 (MQ=255) gCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAgg > 1:2079479/1‑67 (MQ=255) gCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAg < 1:1332417/70‑1 (MQ=255) ggTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGAt > 1:3389492/1‑70 (MQ=255) ttGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATaaa > 1:1381312/1‑71 (MQ=255) ttGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATaaa < 1:3241335/71‑1 (MQ=255) ttGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATaaa > 1:3326829/1‑71 (MQ=255) tgtgTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCa > 1:784414/1‑60 (MQ=255) gtCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGt > 1:2473339/1‑71 (MQ=255) tCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTc < 1:2416006/71‑1 (MQ=255) aCCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCAc > 1:1559311/1‑71 (MQ=255) ccAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATaaa > 1:1175016/1‑63 (MQ=255) ccAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAg < 1:3388718/66‑1 (MQ=255) ccAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAg < 1:3631957/66‑1 (MQ=255) cAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCg > 1:1939040/1‑71 (MQ=255) aaCTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGt > 1:3021618/1‑71 (MQ=255) aaCTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGt > 1:2610967/1‑71 (MQ=255) ggACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCt > 1:156855/1‑71 (MQ=255) gACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATaa < 1:3180661/55‑1 (MQ=255) gACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATaa < 1:3521487/55‑1 (MQ=255) gACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTc < 1:1515770/61‑1 (MQ=255) caacaTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTgc > 1:886825/1‑71 (MQ=255) caTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATa < 1:2383298/53‑1 (MQ=255) caTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTgcgc > 1:1555160/1‑70 (MQ=255) caTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTg > 1:3359904/1‑67 (MQ=255) caTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCa < 1:431267/71‑1 (MQ=255) aTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATaaa < 1:618671/50‑1 (MQ=255) aTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCaa < 1:441345/71‑1 (MQ=255) aTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCaa < 1:499546/71‑1 (MQ=255) ttttCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGt > 1:1515724/1‑53 (MQ=255) tttCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATa > 1:703998/1‑50 (MQ=255) ttCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAg < 1:3645833/70‑1 (MQ=255) ccACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAgg < 1:2295147/69‑1 (MQ=255) cACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGt < 1:1758485/54‑1 (MQ=255) cACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCa < 1:3150948/70‑1 (MQ=255) cACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCAt > 1:1518357/1‑71 (MQ=255) aCGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATg < 1:789255/71‑1 (MQ=255) gCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGgtg < 1:3036538/71‑1 (MQ=255) ccAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTcc < 1:3658690/50‑1 (MQ=255) aaCGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGgtgt < 1:305730/69‑1 (MQ=255) aaCGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCa < 1:54645/71‑1 (MQ=255) cGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATg > 1:1121240/1‑61 (MQ=255) cGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCAt > 1:2485860/1‑70 (MQ=255) ggAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTa > 1:3507228/1‑71 (MQ=255) aaaGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATggg < 1:742987/60‑1 (MQ=255) aaaGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATggg < 1:1347144/60‑1 (MQ=255) aGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTa < 1:2410922/71‑1 (MQ=255) aGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTa < 1:3768739/71‑1 (MQ=255) aGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTa < 1:1096826/71‑1 (MQ=255) aGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTa < 1:2741461/71‑1 (MQ=255) gtgtCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTgcgc < 1:3907594/46‑1 (MQ=255) gtgtCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTgcgc < 1:1051350/46‑1 (MQ=255) tgttgtTCGCCAGGCAGATAATTAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTAc < 1:1924616/62‑1 (MQ=255) tgttgtTCACCAGGCAGATAAATAGTCACCGTTCCTTgcg > 1:1228834/1‑40 (MQ=255) tgttgtTCACCAGGCAGATAAATAGTCACCGTTCCTTgcg > 1:3242917/1‑40 (MQ=255) gttgttCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCa < 1:1985568/41‑1 (MQ=255) gttgttCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCa < 1:2487142/41‑1 (MQ=255) gttgttCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCa < 1:2187009/41‑1 (MQ=255) ttgttCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGt > 1:3842433/1‑62 (MQ=255) ttgttCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGt > 1:2596973/1‑62 (MQ=255) ttgttCACCAGGCATATAAATAGTCACCGTTCCTTGCGCAAAGGCa < 1:873898/46‑1 (MQ=255) ttgttCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCa < 1:1661703/46‑1 (MQ=255) ttgttCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCa > 1:2232734/1‑46 (MQ=255) ttgttCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGc < 1:332244/71‑1 (MQ=255) ttCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACg > 1:483210/1‑67 (MQ=255) | GCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGC > minE/705226‑705359 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |