| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 706,680 | C→T | 48.3% | R244K (AGG→AAG) | lpxL ← | lauryl‑acyl carrier protein (ACP)‑dependent acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 706,680 | 0 | C | T | 48.3% | 3.9 / 80.0 | 60 | R244K (AGG→AAG) | lpxL | lauryl‑acyl carrier protein (ACP)‑dependent acyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (14/17); new base T (17/12); total (31/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGT > minE/706613‑706750 | ggCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTa < 1:155364/70‑1 (MQ=255)ggCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTAc > 1:667509/1‑71 (MQ=255)ggCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTa > 1:1902880/1‑70 (MQ=255) atcatcCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGc < 1:3713973/71‑1 (MQ=255) atcatcCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGc < 1:3179163/71‑1 (MQ=255) tcCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTgg < 1:3051373/71‑1 (MQ=255) tcCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTg > 1:1400883/1‑70 (MQ=255) cAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGaa > 1:2090058/1‑71 (MQ=255) aGTGGCGGAGAACACTCTGGCGTCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAAc < 1:3832297/71‑1 (MQ=255) aGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAAc > 1:2166284/1‑71 (MQ=255) aGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAAc < 1:2280555/71‑1 (MQ=255) gTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACg < 1:314330/71‑1 (MQ=255) tGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGa < 1:64875/71‑1 (MQ=255) tGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGc < 1:3102492/62‑1 (MQ=255) tGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGa < 1:2457902/71‑1 (MQ=255) gCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACg > 1:2756922/1‑68 (MQ=255) gCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGaa < 1:3648848/70‑1 (MQ=255) ggAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGa < 1:2857560/63‑1 (MQ=255) gagaACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGc < 1:3493556/57‑1 (MQ=255) gagaACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGc < 1:1021251/57‑1 (MQ=255) gaACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCAc > 1:3378801/1‑71 (MQ=255) cacTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGaa > 1:1119403/1‑62 (MQ=255) cacTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAg < 1:796941/71‑1 (MQ=255) ctctGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGa > 1:3093766/1‑70 (MQ=255) tctGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGc > 1:79990/1‑49 (MQ=255) tGGCGGCAGCATAATCAATTGATACCTTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATg > 1:1423311/1‑71 (MQ=255) tGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGa < 1:3325833/56‑1 (MQ=255) tGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACa < 1:2599874/69‑1 (MQ=255) gcggcAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCa < 1:33538/63‑1 (MQ=255) gcggcAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATgcg > 1:538476/1‑71 (MQ=255) gcggcAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATgc < 1:1473593/70‑1 (MQ=255) gcagcaTAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAgg < 1:2246336/54‑1 (MQ=255) cagcaTAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCAcc < 1:2916753/58‑1 (MQ=255) gcaTAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAg > 1:31418/1‑58 (MQ=255) aTAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCa > 1:3049575/1‑52 (MQ=255) aTAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCa > 1:882503/1‑52 (MQ=255) aTAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCa > 1:3237078/1‑52 (MQ=255) aTAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCa > 1:3060840/1‑52 (MQ=255) aTAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCa > 1:923435/1‑52 (MQ=255) aTAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACAt < 1:2926149/71‑1 (MQ=255) aaTCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAggg > 1:2058345/1‑48 (MQ=255) aTCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACg < 1:3200515/42‑1 (MQ=255) tCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGAc < 1:1525963/54‑1 (MQ=255) aaTTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGc > 1:1650768/1‑45 (MQ=255) aaTTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGc > 1:1768887/1‑45 (MQ=255) aTACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCa < 1:3672643/71‑1 (MQ=255) aCCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATc > 1:1456431/1‑71 (MQ=255) cccTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATcc < 1:1136347/71‑1 (MQ=255) ccTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATcc < 1:1387124/70‑1 (MQ=255) cTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCAt > 1:2922509/1‑67 (MQ=255) tttGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATgc < 1:3291829/45‑1 (MQ=255) tttGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAgg > 1:615606/1‑71 (MQ=255) tttGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAgg > 1:3504992/1‑71 (MQ=255) tGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGtt > 1:2016051/1‑71 (MQ=255) gCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGtt < 1:939370/70‑1 (MQ=255) aTCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCgg > 1:3764908/1‑71 (MQ=255) aTCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCgg > 1:2478136/1‑71 (MQ=255) tGGCTTACGGCGTGGAACGAAGGGCACCAGAAATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCg > 1:1609612/1‑71 (MQ=255) tGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCg > 1:344947/1‑71 (MQ=255) tGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCg > 1:1730857/1‑71 (MQ=255) cTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGt > 1:3404665/1‑71 (MQ=255) | GGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGT > minE/706613‑706750 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |