| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 720,216 | T→C | 32.0% | intergenic (+14/+221) | mviN → / ← rne | predicted inner membrane protein/fused ribonucleaseE endoribonuclease and scaffold for formation of degradosome |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 720,216 | 0 | T | C | 32.0% | 24.0 / 17.5 | 25 | intergenic (+14/+221) | mviN/rne | predicted inner membrane protein/fused ribonucleaseE endoribonuclease and scaffold for formation of degradosome |
| Reads supporting (aligned to +/- strand): ref base T (9/8); new base C (5/3); total (14/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
CACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGTCTGCAGTGCAGGCCGGAGATAATCTTTTCGCTTTAAAACATATCATGAAACTGGGTATGTTTTGTC > minE/720150‑720282 | cACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGTCtgc < 1:607485/71‑1 (MQ=255)cACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtg < 1:1573393/70‑1 (MQ=255) gCGGTACTTGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagtg < 1:1294940/70‑1 (MQ=255) gCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagtgc < 1:3230532/71‑1 (MQ=255) aCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGTCTGCAGTGCAGGcc > 1:2435195/1‑71 (MQ=255) ggCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGTCtgcagtgc < 1:175774/62‑1 (MQ=255) cAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATaa > 1:482230/1‑70 (MQ=255) cAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATaa > 1:2142042/1‑70 (MQ=255) aGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGTCTGCAGTGCAGGCCGGAGAt < 1:3749155/65‑1 (MQ=255) aGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCgg > 1:3344642/1‑61 (MQ=255) aGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCt > 1:721213/1‑70 (MQ=255) ttAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGTCTGCAGTGCAGGCCGGAGATaa > 1:2835238/1‑65 (MQ=255) ttAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGTCTGCAGTGCAGGCCGGAGATAATCtttt < 1:911173/71‑1 (MQ=255) ttAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagtgcag > 1:631701/1‑54 (MQ=255) aaaGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGTCtgca > 1:2959041/1‑46 (MQ=255) aTTTGCCCGCCGGACGGTGTAACAATGCATTCCGGTCTGCAGTGCAGGCCGGAGATAATCTTTTCGCttt > 1:708286/1‑70 (MQ=255) tGCCCGCCGGACGGTGTAACAATGCATTCCGGTCTGCAGTGCAGGCCGGAGATAATCTTTTCGCTTTaaaa > 1:1125406/1‑71 (MQ=255) cgccgGACGGTGTAACAATGCATTCCGGTCTGCAGTGCAGGCCGGAGATAATCTTTTCGCTTTAAAACata < 1:373740/71‑1 (MQ=255) gccgGACGGTGTAACAATGCATTCCGGTCTGCAGTGCAGGCCGgaga > 1:1614756/1‑47 (MQ=255) ccgGACGGTGTAACAATGCATTCCGGTCTGCAGTGCAGGCCGGAGATAATCtt > 1:1104064/1‑53 (MQ=255) cgGACGGTGTAACAATGCATTCCGGTCTGCAGTGCAGGCCGGAGATAATCTTTTCGCTTTAAAACATATCa > 1:1653247/1‑71 (MQ=255) tgtAACAATGCATTCCGGTCTGCAGTGCAGGCCGGAGATAATCTTTTCGCTTTAAAACATATCATGaaa < 1:422232/69‑1 (MQ=255) tGCATTCCGGTCTGCAGTGCAGGCCGGAGATAATCTTTTCGCTTTAAAACATATCATGAAACTGGGTATGt < 1:108330/71‑1 (MQ=255) cATTCCGGTCTGCAGTGCAGGCCGGAGATAATCTTTTCGCTTTAAAACATATCATGAAACt < 1:433860/61‑1 (MQ=255) ccGGTCTGCAGTGCAGGCCGGAGATAATCTTTTCGCTTTAAAACATATCATGAAACTGGGTATGTTTTGTc > 1:1945392/1‑71 (MQ=255) tCTGCAGTGCAGGCCGGAGATAATCTTTTCGCTTTAAAACATATCATGaaa > 1:425866/1‑51 (MQ=255) | CACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGTCTGCAGTGCAGGCCGGAGATAATCTTTTCGCTTTAAAACATATCATGAAACTGGGTATGTTTTGTC > minE/720150‑720282 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |