| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 741,873 | T→C | 12.2% | Y124Y (TAT→TAC) | ycfL → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 741,873 | 0 | T | C | 12.2% | 102.2 / 6.5 | 41 | Y124Y (TAT→TAC) | ycfL | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (15/21); new base C (3/2); total (18/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
TTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGGGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTC > minE/741807‑741943 | ttCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGGTCGCACAAAGTCAGACTTTATCTATATTTGt < 1:1438884/71‑1 (MQ=255)ttCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGGGCGCACAAAGTCAGACTTTATCTATATTTGt < 1:190783/71‑1 (MQ=255) cggcggTAACGCTGTACGGCAGCGCCAATTTTCTGGGGGCGCACAAAGTCAGACTTTATCTATATTTGt > 1:177853/1‑69 (MQ=255) cggcggTAACGCTGTACGGCAGCGCCAATTTTCTGGGGGCGCACAAAGTCAGACTTTATCTATATTTGt > 1:2670828/1‑69 (MQ=255) cggcggTAACGCTGTACGGCAGCGCCAATTTTCTGGGGGCGCACAAAGTCAGACTTTATCTATATTTGTa < 1:2700431/70‑1 (MQ=255) gcggTAACGCTGTACGGCAGCGCCAATTTTCTGGGGGCGCACAAAGTCAGACTTTATCTATATTTGTAAgg < 1:3311783/71‑1 (MQ=255) tGTACGGCAGCGCCAATTTTCTGGGGGCGCACAAAGTCAGACTTTATCTATATTTGTAAggg > 1:3189512/1‑62 (MQ=255) tGTACGGCAGCGCCAATTTTCTGGGGGCGCACAAAGTCAGACTTTATCTATACTTGTAAGGGGTTAATCtt > 1:348066/1‑71 (MQ=255) gTACGGCAGCGCCAATTTTCTGGGGGCGCACAAAGTCAGACTTTATCTATATTTGTAAggg < 1:996971/61‑1 (MQ=255) gTACGGCAGCGCCAATTTTCTGGGGGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCtt > 1:25091/1‑70 (MQ=255) tACGGCAGCGCCAATTTTCTGGGGGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTtga > 1:770832/1‑71 (MQ=255) cGGCAGCGCCAATTTTCTGGGGGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTtgat < 1:3828999/70‑1 (MQ=255) cGGCAGCGCCAATTTTCTGGGGGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTtgat < 1:1255771/70‑1 (MQ=255) aGCGCCAATTTTCTGGGGGCGCACAAAGTCAGACTTTATCTATATTTGTaa > 1:2766518/1‑51 (MQ=255) cgcCAATTTTCTGGGGGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACaaaa < 1:1216523/71‑1 (MQ=255) ccAATTTTCTGGGGGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAAt < 1:936020/71‑1 (MQ=255) cAATTTTCTGGGGGCGCACAAAGTCAGACTTTATCTATACTTGt < 1:2464113/44‑1 (MQ=255) aTTTTCTGGGGGCGCACAAAGTCAGACTTTATCTATATTTGTaa > 1:2321391/1‑44 (MQ=255) aTTTTCTGGGGGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAg < 1:62784/71‑1 (MQ=255) tGGGGGCGCACAAAGTCAGACTTTATCTATACTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTa > 1:3719145/1‑71 (MQ=255) gggCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGt < 1:2807340/40‑1 (MQ=255) gcgcACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCt < 1:3327637/71‑1 (MQ=255) gcACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGaa > 1:2098241/1‑39 (MQ=255) gcACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGaa > 1:251041/1‑39 (MQ=255) acaAAGTCAGACTTTATCTATATTTGTAAGGGGTGAAt < 1:1753250/38‑1 (MQ=255) caAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGAtt < 1:2671302/71‑1 (MQ=255) aaaGTCAGACTTTATCTATATTTGTAAGGGGTTAAt < 1:1061602/36‑1 (MQ=255) aaaGTCAGACTTTATCTATACTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTa > 1:3710508/1‑71 (MQ=255) aaGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTAc > 1:2120750/1‑71 (MQ=255) aaGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTAc > 1:1901098/1‑71 (MQ=255) aGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTAcc < 1:3370963/71‑1 (MQ=255) tCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTc < 1:219827/52‑1 (MQ=255) tCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTg < 1:767438/63‑1 (MQ=255) aGACTTTATCTATACTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCg < 1:2667697/51‑1 (MQ=255) aCTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTa > 1:3213847/1‑63 (MQ=255) tttATCTATATTTGTAAGGGGTGAATCTTGATGGCAAAAATGAGTCGCTACGCCTTGATTACCGCGCTgg > 1:655555/1‑70 (MQ=255) tttATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTgg > 1:2383108/1‑70 (MQ=255) tATCTATATTTGTAAGGGGTGAATCTTGATGACaa > 1:2149536/1‑35 (MQ=255) tATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGc < 1:3426848/69‑1 (MQ=255) tATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGc < 1:1850994/69‑1 (MQ=255) tCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGAt < 1:2086760/70‑1 (MQ=255) tatTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGt < 1:2056640/68‑1 (MQ=255) tttGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTtctc > 1:2956081/1‑71 (MQ=255) | TTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGGGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTC > minE/741807‑741943 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |