| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 775,475 | C→T | 10.9% | H366H (CAC→CAT) | icd → | isocitrate dehydrogenase, specific for NADP+ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 775,475 | 0 | C | T | 10.9% | 150.2 / 8.0 | 55 | H366H (CAC→CAT) | icd | isocitrate dehydrogenase, specific for NADP+ |
| Reads supporting (aligned to +/- strand): ref base C (23/26); new base T (4/2); total (27/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
AATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAA > minE/775408‑775545 | aaTATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATg < 1:3244265/71‑1 (MQ=255) atatGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATgg > 1:2080564/1‑71 (MQ=255) atatGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATgg > 1:3271018/1‑71 (MQ=255) atatGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATgg > 1:3924770/1‑71 (MQ=255) tatGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATggg < 1:2697726/71‑1 (MQ=255) tatGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATggg < 1:796021/71‑1 (MQ=255) atGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATggg < 1:32292/70‑1 (MQ=255) atGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGt > 1:2085816/1‑71 (MQ=255) tGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGtt > 1:2893131/1‑71 (MQ=255) tGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGtt > 1:88874/1‑71 (MQ=255) tGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGt < 1:734120/70‑1 (MQ=255) tGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGt < 1:1839639/70‑1 (MQ=255) cGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGa > 1:218588/1‑71 (MQ=255) gTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGAcc > 1:3059513/1‑71 (MQ=255) gTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGAcc < 1:2267106/71‑1 (MQ=255) gTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGAcc > 1:3802079/1‑71 (MQ=255) tCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTCCGCCACATGGGTTGGACCg < 1:2033961/71‑1 (MQ=255) ggACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACAt < 1:1392972/57‑1 (MQ=255) ggACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAg < 1:2203892/71‑1 (MQ=255) aCAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCg < 1:1138752/71‑1 (MQ=255) cAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCgg > 1:714894/1‑71 (MQ=255) cAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCg > 1:2753179/1‑70 (MQ=255) aaaGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGc < 1:1649686/71‑1 (MQ=255) aGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCcaca > 1:1084858/1‑50 (MQ=255) aGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCcaca > 1:3587809/1‑50 (MQ=255) aGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGt > 1:1573541/1‑55 (MQ=255) tAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGa > 1:3629737/1‑57 (MQ=255) aaaTCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACt > 1:1124496/1‑71 (MQ=255) aaaTCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACt > 1:3263982/1‑71 (MQ=255) cTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTg < 1:777707/63‑1 (MQ=255) ctctATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTa < 1:3803664/71‑1 (MQ=255) tattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTaa < 1:3346182/69‑1 (MQ=255) ttattCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAg > 1:1041134/1‑48 (MQ=255) ttattCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAg > 1:1626294/1‑48 (MQ=255) ctctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATgg < 1:1377093/70‑1 (MQ=255) ctctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATgg < 1:3127539/70‑1 (MQ=255) tcCGCTGAGATGATGCTGCGCCACATGTGTTGGACCGAAg > 1:607895/1‑40 (MQ=255) tcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGa < 1:1239885/68‑1 (MQ=255) tcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGa < 1:1499814/68‑1 (MQ=255) cGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAg < 1:2283565/68‑1 (MQ=255) cGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGgcg > 1:3301351/1‑71 (MQ=255) cTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATgg > 1:1714068/1‑63 (MQ=255) tGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCaa > 1:3162991/1‑71 (MQ=255) gatgatGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCa < 1:2997594/71‑1 (MQ=255) gacgatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCa < 1:2011454/68‑1 (MQ=255) ctaatgCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaa < 1:3770846/68‑1 (MQ=255) atgatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCaa < 1:1334831/71‑1 (MQ=255) tgatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCaa < 1:164725/70‑1 (MQ=255) gatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTaa < 1:3803803/48‑1 (MQ=255) tgctgcGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTa > 1:2101086/1‑45 (MQ=255) tgctgcGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgcc < 1:2660882/71‑2 (MQ=255) tgctgcGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgc > 1:3095905/1‑70 (MQ=255) tgctgcGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgc > 1:1458380/1‑70 (MQ=255) ctgcGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGaa < 1:236511/54‑1 (MQ=255) cgcCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATgg > 1:339422/1‑49 (MQ=37) cgcCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAg > 1:352914/1‑43 (MQ=255) gcCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAAc > 1:3032286/1‑62 (MQ=255) acaTGGGTTGGACCGAAGCGGCTGACTTAATTGTTaaa < 1:3699681/38‑1 (MQ=255) caTGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTaa > 1:890819/1‑71 (MQ=255) | AATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAA > minE/775408‑775545 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |